Acetylated tubulin
Inclusion Body Fusion of Human Parainfluenza Virus Type 3 Regulated by Acetylated α-Tubulin Enhances Viral Replication
... with acetylated ␣ -tubulin, IBs colocalize with acetylated ␣ -tubulin, and acetylated ␣ -tubulin promotes the fusion of small IBs into large IBs, thereby enhancing viral RNA ...
16
Histone Deacetylase 3 indirectly modulates tubulin acetylation
... of acetylated tubulin between control, KD and eGFP–HDAC3-expressing cells (Figure 6A), suggesting that, whereas HDAC3 may play a role in deacetylation of microtubules in interphase cells, it may not have ...
11
The diversity of acetylated proteins
... flagellar tubulin from the unicellular alga Polytomella [38]. Tubulin acetylation has since been observed in verte- brates, insects, nematodes and plants, in all of which the acetyl group is attached to the ...
6
Mesodermal ALK5 controls lung myofibroblast versus lipofibroblast cell fate
... against acetylated tubulin, CC10, pro-SPC, and T1a (specific to ciliated cells, Clara cells, alveolar epithelial type II cells, and alveolar epithelial type 1 cells, respectively) showed reduced ...
15
Screen-based identification and validation of four new ion channels as regulators of renal ciliogenesis
... For immunostaining, mIMCD3 cells were grown on coverslips and fixed for 5 min in ice-cold methanol and blocked 60 min in 1% bovine serum albumin (BSA). Primary antibody incubations (mouse anti-acetylated ...
10
Screen based identification and validation of four new ion channels as regulators of renal ciliogenesis
... For immunostaining, mIMCD3 cells were grown on coverslips and fixed for 5 min in ice-cold methanol and blocked 60 min in 1% bovine serum albumin (BSA). Primary antibody incubations (mouse anti-acetylated ...
10
Trypanosoma cruzi Bromodomain Factor 3 Binds Acetylated α-Tubulin and Concentrates in the Flagellum during Metacyclogenesis
... (CFP). Tubulin can acquire different post- translational modifications, which modulate microtubule ...functions. Acetylated ␣ -tubulin has been found in the axonemes of flagella and cilia, as well as ...
10
Sonic hedgehog signalling as a potential endobronchial biomarker in COPD
... (Acetylated tubulin, green); Gli2 (Gli2, red); basal cells (p63, white) and cell nuclei (DAPI, blue) in both non-COPD (upper panel) and COPD patients (lower ...
11
Targeting microtubule alterations in axon degeneration and Alzheimer’s Disease
... whether acetylated tubulin is the outcome of stabilised microtubules, or is a process required to stabilise ...on acetylated-rich microtubules (Sudo and Baas, ...than tubulin dimers (Kull and ...
299
Nitric oxide in control of luminescence from hatchetfish (Argyropelecus hemigymnus) photophores
... List of abbreviations acetylated tubulin-like immunoreactivity dibutyrylguanosine 5′,5′-cyclic monophosphate cyclic guanosine monophosphate TLmax control preparations–TLmax treated prepa[r] ...
11
Ciliogenesis is regulated by a huntingtin HAP1 PCM1 pathway and is altered in Huntington disease
... anti–N-acetylated tubulin Abs as described above, washed in PBS, incubated with horse anti-rabbit FITC conjugate (1:500; Vector) and donkey anti-mouse Cy3 conjugate (1:500; Jackson Immunoresearch ...
12
Pironetin reacts covalently with cysteine 316 of α tubulin to destabilize microtubule
... on tubulin heterodimer in solution (without the other proteins used to obtain crystals) to ensure the modification of Cys316 was not the result of crystal packing or an artefact of T2R-TTL protein complex ...
9
Differential analysis of quantitative proteome and acetyl-proteome profiling between premenopausal and postmenopausal ovarian tissues
... and acetylated proteomes by large-scale LC–MS/MS with TMT ...3047 acetylated sites in 1583 proteins were identified, as well as quantified 3551 proteins and 2256 acetylated sites in 1248 proteins, ...
12
PlexinA4 is necessary as a downstream target of Islet2 to mediate Slit signaling for promotion of sensory axon branching
... Anti-acetylated α-tubulin antibody staining of 26 hpf embryos of the transgenic zebrafish B, which expresses dnPlexinA4-GFP fusion protein in the Rohon-Beard neurons black circles, showe[r] ...
11
Maize Stalk As Reinforcement In Natural Rubber Composites
... Curing refers to heat treatment which changes the physical properties of a substance due to a chemical process, resulting in the formation of a more durable product. Cure time of natural rubber composites decreased with ...
9
An Innovation in the Cancer Therapy by the Interaction of Docetaxel to the Demethylpodophyllotoxin site of Tubulin
... Demethylpodophyllotoxin-treated tubulin show a small reduction in 25-S to 30-S peaks at ...of tubulin is seen at 0° but disappears if the temperature of tubulin incubated with Demethylpodophyllotoxin ...
8
The role of tubulin tubulin lattice contacts in the mechanism of microtubule dynamic instability
... RESULTS Determination of 13-PF structures of DCX-MTs in different nucleotide states To elucidate the mechanism of MT catastrophe, we analysed conformational changes in tubulin through it[r] ...
32
Antimitotic herbicides bind to an unidentifed site on malarial parasite tubulin and block development of liver stage Plasmodium parasites
... Protein affinity assay using the tubulin fusion proteins.. The affinity of the tubulin fusions for other proteins was determined by their ability to.[r] ...
30
Novel peptide–dendrimer conjugates as drug carriers for targeting nonsmall cell lung cancer
... carrier, the LCTP and fluorescence-labeled molecule (FITC) were conjugated to an acetylated polyamidoamine (PAMAM) dendrimer of generation 4 (G4) to form a PAMAM–Ac–FITC– LCTP conjugate. The performance of the ...
11
A proteomic approach to analyze the aspirin mediated lysine acetylome
... Two active biological processes will impact on the rate of decay of an acetylation signal in cultured cells; the rate of protein turnover, and the action of cellular lysine deacetylases (KDACs). Two proteomic studies ...
17