Acyl-CoA synthetase activity
Functions of rat acyl-CoA synthetases in bacteria and mammalian cells
... chain acyl-CoA synthetases ...The acyl-CoA synthetase activity of FATP and ACSL may contribute to LCFA uptake by trapping LCFA in the form of LC-CoA upon ...provide ...
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Intrinsic acyl-CoA thioesterase activity of a peroxisomal ATP binding cassette transporter is required for transport and metabolism of fatty acids.
... C8:0 acyl-CoA synthetase activity was ...(3-HAD) activity was used as a marker for peroxisomes Data are expressed as a percentage of the total ...C8:0-CoA synthetase ...
5
Acyl-CoA synthetase 1 exacerbates lipotoxic inflammasome activation. in primary macrophages
... To determine whether ACSL1 influenced crystal formation in response to palm-LPS we assessed ORO staining and quantification in WT and mACSKO macrophages. Consistent with our lysosome damage data, crystal formation was ...
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Improved Characterization of Polyunsaturated Fatty Acids Desaturases and Elongases by Co-Expression in Saccharomyces cerevisiae with a Protozoan Acyl-CoA Synthetase
... action of the endogenous ACS and a marginal activity of ACS1. By contrast, intake of AA appeared to be mainly conducted by the heterologous ACS1, in good agreement with its known substrate specificity. The uptake ...
15
Fatty Acid Oxidation Mediated by Acyl-CoA Synthetase Long Chain 3 Is Required for Mutant KRAS Lung Tumorigenesis
... catalytic activity is required for the vulnerability to ACSL3 depletion in mutant KRAS ...the activity of several oncogenes, including KRAS ( Gysin et ...long-chain acyl-CoAs cannot be replenished by ...
16
A role for long-chain acyl-CoA synthetase-4 (ACSL4) in diet-induced phospholipid remodeling and obesity-associated adipocyte dysfunction.
... 2.4. Adipocyte isolation Gonadal adipose fat pads were dissected from the mouse, weighed, and immediately submerged in Krebs-Ringer bicarbonate (KRB) buffer at pH 7.4 with 4% bovine serum albumin (BSA), 500 nM adenosine, ...
15
Metabolic Regulation of Histone Acetyltransferases by Endogenous Acyl-CoA Cofactors
... metabolic acyl-CoAs are capable of inhibiting KAT-catalyzed acetylation with some degree of ...metabolic acyl-CoAs, thereby producing endogenous KAT bisubstrate inhibitors in ...to CoA analogs via ...
11
Acyl-CoA synthetase-4, a new regulator of mTOR and a potential therapeutic target for enhanced estrogen receptor function in receptor-positive and -negative breast cancer
... ACSL4 cells [4], strongly suggest that rosiglitazone effects are due to the inhibition of ACSL4 activity. It has been suggested that the increased activation of mTOR, possibly through the PI3K/AKT pathways, plays ...
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Intrinsic acyl-CoA thioesterase activity of a peroxisomal ATP binding cassette transporter is required for transport and metabolism of fatty acids.
... and synthetase is also reflected in a physical interaction in ...thioesterase activity which is stimulated by ATP and is essential for fatty acid degradation in yeast and in ...
7
Using the pimeloyl CoA synthetase adenylation fold to synthesize fatty acid thioesters
... the acyl-chain length specificity 18 ...with acyl-ACPs of varying chain lengths have provided a mechanistic understanding of how heme chemistry is used to act upon the acyl chain and generate pimelic ...
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ab Acyl-CoA Synthetase Assay Kit (Fluorometric)
... ab273315 Acyl-CoA Synthetase Assay Kit (Fluorometric) 6 8. Technical Hints This kit is sold based on number of tests. A “test” simply refers to a single assay well. The number of wells that contain ...
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The Maize High-Lysine Mutant opaque7 Is Defective in an Acyl-CoA Synthetase-Like Protein
... The putative o7-ref allele has a 12-bp in-frame deletion of codons 350 – 353 in a 528-codon-long acyl-CoA synthetase-like gene (ACS). We then con fi rmed this candidate gene by generating another ...
16
Mycobacterial Acyl-CoA Corboxylases.
... in acyl-CoA carboxylase have not been ...the activity of this enzyme is probably essential for growth (Perez et a l, ...final activity of the enzyme would be influenced by the ratio of active ...
176
The effect of acyl-CoA synthetase long-chain family member 5 on triglyceride synthesis in bovine preadipocytes
... Abstract. Acyl-CoA synthetase long-chain family member 5 (ACSL5) is a member of the acyl coenzyme A (CoA) long-chain synthase families (ACSLs), and it plays a key role in fatty acid ...
8
Acyl-CoA synthetase isoform 1 deficiency impairs beta-oxidation in mouse heart and adipose tissue
... of acyl- CoAs that are used for β-oxidation. If all acyl-CoAs were metabolically equivalent, one would expect that the rates of acyl-CoA incorporation into all metabolic end products would be ...
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Structural Basis of the Substrate-specific Two-step Catalysis of Long Chain Fatty Acyl-CoA Synthetase Dimer* □S
... fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regu- lation of cellular functions via the production of long chain fatty acyl-CoA ...fatty ...
10
Structure of p300 in complex with acyl-coa variants
... diverse acyl-CoA cofactors in the active site in a conformation that is compatible with the binding of protein substrates and transfer of the acyl moiety, we have systematically analyzed ...
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SIRT3 and SIRT5 regulate the enzyme activity and cardiolipin binding of very long-chain Acyl-CoA dehydrogenase
... * [email protected] Abstract SIRT3 and SIRT5 have been shown to regulate mitochondrial fatty acid oxidation but the molecular mechanisms behind the regulation are lacking. Here, we demonstrate that SIRT3 and SIRT5 ...
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Structural and Functional Studies on Acyl-CoA Carboxylases of Mycobacterium tuberculosis
... Zusammenfassung Mycobacterium tuberculosis ist der Tuberkuloseerreger, dem jährlich zwei Millionen Menschen zum Opfer fallen und der jedes Jahr viele Millionen Neuinfektionen aufweist. Die Zellwand von M. tuberculosis ...
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Structural and Kinetic studies of Acyl-CoA Carboxylases in Mycobacterium tuberculosis
... The requirement of mycolic acids for the growth, survival and antibiotic resistance of Mycobacteria means the enzymes involved in mycolic acid synthesis are attractive potential drug targets. Acyl-CoA ...
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