Adeno-associated viruses
Mapping and Engineering Functional Domains of the Assembly-Activating Protein of Adeno-associated Viruses
... IMPORTANCE Adeno-associated viruses (AAVs) encode a unique assembly-activating protein (AAP) within their genomes that is essential for capsid ...
18
Sequence Properties of the MAAP Protein and of the VP1 Capsid Protein of Adeno-Associated Viruses
... Abstract Adeno-associated viruses (AAVs, genus dependoparvovirus) are promising gene therapy vectors. In strains AAV1-12, the capsid gene VP1 encodes a recently discovered protein, MAAP, in an ...
20
Molecular Characterization of Adeno-Associated Viruses Infecting Children
... To extend our understanding of wild-type AAV infection in humans, we set out to characterize AAV genomes directly out of freshly acquired human tissues. Because our assumption was that many (if not all) AAV infections ...
12
Pathways of Cell Infection by Parvoviruses and Adeno-Associated Viruses
... Animal viruses have developed various strategies for infect- ing cells, and all begin with adsorption to cell surface receptors, penetration into the cytosol, uncoating or release of the viral genome, and ...
6
Clades of Adeno-Associated Viruses Are Widely Disseminated in Human Tissues
... using Adeno-associated virus (AAV) as a vector for human gene therapy has stimulated interest in the Dependovirus ...of viruses and viral sequences from clinical samples are extremely ...
8
Sequence Properties of the MAAP Protein and of the VP1 Capsid Protein of Adeno-Associated Viruses
... Alignment of the nucleotide sequence coding for the region of VP1 located between the start codons of MAAP and AAP in porcine AAVs, in PFAM format. Conventions are the same as in Fig S3A[r] ...
15
Characterization of the Transcription Profile of Adeno-Associated Virus Type 5 Reveals a Number of Unique Features Compared to Previously Characterized Adeno-Associated Viruses
... Jianming Qiu, Ramnath Nayak, Gregory E. Tullis, and David J. Pintel* School of Medicine, University of Missouri-Columbia, Columbia, Missouri 65212 Received 3 July 2002/Accepted 11 September 2002 We report the initial ...
13
In Vitro and In Vivo Gene Therapy Vector Evolution via Multispecies Interbreeding and Retargeting of Adeno-Associated Viruses
... In conclusion, DNA family shuffling may be the most pow- erful and potent library-based AAV evolution method to date. With its hallmark of recombination of functional genes, it is an ideal tool to molecularly breed novel ...
25
Helper-free stocks of recombinant adeno-associated viruses: normal integration does not require viral gene expression.
... For further confirmation that no helper virus was present in stocks of AAV/neo prepared with pAAV/Ad as helper DNA, a stock of the recombinant virus was subjected to equilibrium density [r] ...
7
Adeno-Associated Viruses Can Induce Phosphorylation of eIF2α via PKR Activation, Which Can Be Overcome by Helper Adenovirus Type 5 Virus-Associated RNA
... Received 24 May 2007/Accepted 9 August 2007 Mutants of adenovirus type 5 (Ad5) virus-associated RNA I deficient in inhibiting the activation and subsequent phosphorylation of protein kinase R (PKR) could neither ...
9
Expression Profiles of Bovine Adeno-Associated Virus and Avian Adeno-Associated Virus Display Significant Similarity to That of Adeno-Associated Virus Type 5
... nonprimate-derived adeno-associated viruses, namely, bovine adeno-associated virus (B-AAV) and avian adeno-associated virus (A-AAV), which were obtained after the ...
12
Adeno-Associated Viral Vectors
... Background Adeno-associated viruses (AAV) are single strand DNA viruses that require a helper virus (typically adenovirus, herpesvirus and vaccinia) to ...These viruses are infectious ...
5
Gangliosides Are Essential for Bovine Adeno-Associated Virus Entry
... 14). Adeno-associated viruses are members of the genus De- pendovirus, a small group of viruses that were classified based on similar size and structure and dependence upon a helper virus for ...
7
Interference Between Two Adeno-associated Satellite Viruses: a Three-Component System
... CF data also showed that the yield of type 1 satellite was inhibited until type 4 was added, 12 or more hr after simultaneous inoculation of SV15 and type 1 satellite, evidence for inter[r] ...
6
Infectious Entry Pathway of Adeno-Associated Virus and Adeno-Associated Virus Vectors
... (yellow signal). This may be indicative of a mechanism of internalization requiring the grouping of multiple receptor- AAV complexes on the surface of the host cell prior to entry. Translocation of AAV to the nucleus. ...
9
Genetics of adeno-associated virus: isolation and preliminary characterization of adeno-associated virus type 2 mutants.
... Mutants with mutations between 48 and 55 map units synthesized normal amounts of AAV single-stranded and duplex DNA but produced substantially lower yields of infectious virus particles [r] ...
11
Adeno-associated virus vector integration junctions.
... Department of Medicine and Markey Molecular Medicine Center, University of Washington, Seattle, Washington 98195 Received 29 April 1997/Accepted 31 July 1997 Vectors derived from adeno-associated virus ...
8
In vitro replication of adeno-associated virus DNA.
... (A) NE DNA was incubated with S100 extracts from either uninfected or Ad-infected HeLa cells in the presence of no additional extract, a wild-type baculovirus (wt bac) nuclear extract, o[r] ...
11
Structure of Adeno-Associated Virus Type 4
... III viruses might result from capsid protein trans- lation and posttranslational modification ...I viruses are approximately 50% identical at the amino acid level (except for CPV and FPV, with ⬃ 98% ...
12
Cloning and Characterization of a Bovine Adeno-Associated Virus
... Classical differentiation of viruses relies on a lack of cross- reacting antibodies in serological studies. Therefore, to test for serological identity, rAAV-4 and rBAAV were titered on COS cells in the presence ...
8