Adipose Derived Stromal Cells
Mechanical loading of adipose derived stromal cells causes cell alignment
... that adipose tissue is a signifi- cant source of multipotent cells which can be differenti- ated into cartilage cells ...stem cells instead of chondrocytes shows promise in the treatment of ...
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Intra articular delivery of adipose derived stromal cells attenuates osteoarthritis progression in an experimental rabbit model
... stem cells (MSC) from bone marrow or other tissue sites, based on their capacity to influence and regulate different stages of cartilage repair, is a challenge of considerable appeal to ...including adipose ...
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Culture expansion of adipose derived stromal cells A closed automated Quantum Cell Expansion System compared with manual flask based culture
... Adipose derived stromal cells P0 and P1 culture expanded in a Quantum system or flasks were analyzed by flow cytometry (Navios, Beckman ...Coulter). Cells from T75 flasks were detached ...
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Identical effects of VEGF and serum deprivation on phenotype and function of adipose derived stromal cells from healthy donors and patients with ischemic heart disease
... counted. Cells were re-suspended in flow cytometry-PBS, (1.5×10 5 cells/100 μl) and incu- bated with or without initially titrated antibodies for 15 ...The cells were then centrifuged and ...
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A prospective, non randomized, no placebo controlled, phase Ib clinical trial to study the safety of the adipose derived stromal cells stromal vascular fraction in idiopathic pulmonary fibrosis
... Despite relative enthusiasm arising from our safety data, our trial is underpowered and exhibits a number of caveats that should be addressed cautiously before rigid conclusions can be drawn. This trial has not been ...
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Intravenous administration of adipose derived stromal cells does not ameliorate bleomycin induced lung injury in rats
... lining cells and confluent interstitial inflammatory cell ...the stromal cell treatment was initiated two weeks after bleomycin administration when the fibro- blasts had already started to appear and ...
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Transplantation of modified human adipose derived stromal cells expressing VEGF165 results in more efficient angiogenic response in ischemic skeletal muscle
... of cells there are certain issues concerning their derivation and preparation prior to ...stem cells application is doubtful due to ethical reasons, potential risks of tera- togenesis and immune response to ...
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Autologous transplantation of adipose derived stromal cells ameliorates ventilator induced lung injury in rats
... epithelial cells. Bone marrow (BM)-derived stem cells have been the primary source of stem cells for tissue engineering applica- tions [18]; however, isolation of BM-MSCs requires bone marrow ...
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Disturbed angiogenic activity of adipose derived stromal cells obtained from patients with coronary artery disease and diabetes mellitus type 2
... mesenchymal stromal cells (MSC) have been used in many animal and human studies and serve as an attractive candidate for tissue engineering and cell- based ...be derived from different tissue ...
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Intra articular injection in the knee of adipose derived stromal cells (stromal vascular fraction) and platelet rich plasma for osteoarthritis
... isolated cells may represent a safe and effica- cious way to manage patients with ...planted cells such as pre-aging (telomere shortening), reduced viability, or dedifferentiation/reprogramming that is ...
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Autologous adipose derived stromal cell treatment for patients with refractory angina (MyStromalCell Trial): 3 years follow up results
... In this study, the total amount of ASCs reached were injected into the patients allocated to the active therapy group, while the adipose tissue obtained from some of the patients in the placebo group, were used ...
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Characterization of adipose-derived stem cells from subcutaneous and visceral adipose tissues and their function in breast cancer cells
... MCF-7 cells and even weakly inhibit expansion of MDA-MB-231 ...MDA-MB-231 cells could be due to their receptor composition, the negative expression of ER, PR and HER2 [16], in line with the notion that ...
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Differential activation of Fyn kinase distinguishes saturated and unsaturated fats in mouse macrophages
... increased adipose tissue activated ...diet-induced adipose tissue macrophage ...marrow- derived macrophages. Similarly, Fyn activity is increased in cells of adipose tissue ...
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PDGFRα controls the balance of stromal and adipogenic cells during adipose tissue organogenesis
... excess adipose tissue to the extent that it creates a risk for insulin resistance, type 2 diabetes and other ...how adipose tissue develops and changes over time is essential to identifying new biomarkers ...
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Estrogen deficiency stimulates B lymphopoiesis in mouse bone marrow
... marrow-derived stromal cells (ST2) were pretreated with estrogen then co-cultured with bone marrow cells in the presence of estrogen, the stromal ...
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Characterisation and comparison of adipose tissue macrophages from human subcutaneous, visceral and perivascular adipose tissue
... is upregulated during the late stages of monocyte dif- ferentiation [28] and serves as a scavenger receptor that binds different ligands, including oxidized low-density lipoprotein, oxidized phospholipids, long chain ...
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Application of Hypoxic Exposure Combined with Osteogenic Induction for the Enhancement of Multiple Osteoinductive Capabilities in Rat Mesenchymal Cells
... Total RNA was extracted from the cells using an RNeasy mini kit (Qiagen, Va- lencia, CA, USA), with DNase I treatment (Qiagen). RT-qPCRs were carried out using the TaqMan Fast Virus 1-Step Master Mix (Thermo ...
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Neutrophils trigger a NF-κB dependent polarization of tumor- supportive stromal cells in germinal center B-cell lymphomas
... in stromal cells whereas macrophage migration inhibitory factor (MIF) expression was not ...with stromal cells and this crosstalk should be considered, in addition to the previously ...
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Exosomes secreted by adipose derived mesenchymal stem cells regulate type i collagen metabolism in fibroblasts from women with stress urinary incontinence
... Methods: Periurethral vaginal wall tissues of postmenopausal women with or without SUI were collected during transvaginal surgical procedures. Primary fibroblasts were cultured from periurethral vaginal wall tissues, and ...
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Pregnancy associated breast cancers are driven by differences in adipose stromal cells present during lactation
... T1-12B cells on a given day. 4 T1-12B cells were re- suspended in 3:1 media/Matrigel solution and injected at a total of 5,000 cells per gland on the first day of each developmental ...1E-F) ...
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