Agrobacterium rhizogenes
Production of Hairy Roots by Two Different Strains of Agrobacterium rhizogenes in Castor (Ricinus communis L.) Plant
... 2012). Agrobacterium rhizogenes-mediated gene transfer is being widely used to obtain hairy root lines with high production of useful metabolites (Canel et ...2012). Agrobacterium-mediated hairy ...
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Influence of Agrobacterium rhizogenes strains on hairy roots induction in Trigonella foenum-graecum L. and secondary metabolites production
... by Agrobacterium rhizogenes are a suitable tissue for the production of secondary metabolites, due to the stability and high production of roots without phytohormone in a short ...of Agrobacterium ...
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An improved method for Agrobacterium rhizogenes-mediated transformation of tomato suitable for the study of arbuscular mycorrhizal symbiosis
... Background: Solanum lycopersicum, an economically important crop grown worldwide, has been used as a model for the study of arbuscular mycorrhizal (AM) symbiosis in non‑legume plants for several years and several cDNA ...
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An Optimized Protocol for Agrobacterium rhizogenes-Mediated Genetic Transformation of Citrullus colocynthis
... Introduction: Citrullus colocynthis is considered as a precious herb due to its medicinal and nutritional values and also for its ability to produce valuable bioactive compounds such as cucurbitacin E and quercetin. The ...
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Rapid, Efficient and High Performance Protocol for Agrobacterium rhizogenes Mediated Hairy Root Transformation of the Common Bean Phaseolus vulgaris
... bean, Agrobacterium rhizogenes-mediated trans- formation was used as an alternative method to obtain transgenic roots to study root ...A. rhizogenes-mediated transformation, “composite plants” can be ...
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Establishment of rapid propagation cultures of Agrobacterium rhizogenes – mediated transgenic hairy roots in sugarbeet
... 2014). Agrobacterium rhizogenes mediated gene transfer is widely used to obtain hairy roots in many plant species as in carrot (AL-Mallah and Mohammed, 2012), Fenugreek (AL-Mahdawe et ...
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Agrobacterium rhizogenes-induced soybean hairy roots versus Soybean mosaic virus (ARISHR-SMV) is an efficient pathosystem for studying soybean–virus interactions
... An Agrobacterium rhizogenes (A. rhizogenes) induced soybean hairy roots versus Soybean mosaic virus (ARISHR-SMV) pathosystem was ...A. rhizogenes K599 using soybean cotyledons as explants, (2) ...
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Influence of cotyledon, hypocotyl extracts and authentic andrographolide on selective Agrobacterium rhizogenes strains growth: A deterrent to hairy root induction in Andrographis paniculata (Burm.f.) Wall. ex Nees
... through Agrobacterium rhizogenes mediated genetic ...A. rhizogenes 2364 strain by cut method using cotyledon ...A. rhizogenes bacterial ...A. rhizogenes strains such as 532, 2364, 8196 ...
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Genotype-independent Agrobacterium rhizogenes-mediated root transformation of chickpea: a rapid and efficient method for reverse genetics studies
... A. rhizogenes has emerged as an alternative to traditional transformation and breeding strategies that is gaining importance as an important tool for reverse genetics studies in plants, especially legumes ...A. ...
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The detection of plasmid transfer genes in Rhizobium species : a thesis presented in partial fulfilment of the requirement for the degree Master of Science in Microbiology at Massey University, New Zealand
... The wells were loaded as follows : 1 200ng 1kb, 2 1µg Agrobacterium tumefaciens strain LMG64, 3 lµg Agrobacterium rhizogenes strain ATCC15834, 4 lµg Rhizobium loti strain ATCC33669, 5 lµ[r] ...
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Agrobactirium rhizogenes induced hairy root development and its effect on production of glycyrrhizin in abrus precatorius (l)
... Phytomonas rhizogenes which was later renamed as Agrobacterium ...A. rhizogenes was made by Ackermann ...hybridization. Agrobacterium rhizogenes brings about transformation in plant ...
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ANALYSIS OF TARAXEROL AND TARAXASTEROL IN HAIRY ROOT CULTURES OF TARAXACUM OFFICINALE WEBER
... By HPLC analysis, the quantity of taraxasterol in natural root extract was found to be 2.96 µg/ml and 1.686 µg/ml for taraxerol Fig. 8. In contrast root callus cultures, R1 (MS + IAA + BA + 2, 4-D) (0.5ppm + 1 ppm + 0.5 ...
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Mutagenesis of seed storage protein genes in Soybean using CRISPR/Cas9
... Objective: Soybean seeds are an important source of vegetable proteins for both food and industry worldwide. Con- glycinins (7S) and glycinins (11S), which are two major families of storage proteins encoded by a small ...
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Effect of Agrobacterium on growth and development of Vigna mungo L
... species was isolated from the soil samples of six different leguminous crop areas namely Kudikkadu, Thandangorai, Nadium, Athirampattinum, Buthalur, and Kattuthottam in Thanjavur Dist by using yeast extract mannitol agar ...
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High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes
... Our observations were consistent with literature data (David and Tempé 1988; Puddephat et al. 2001) con- cerning the morphology of plants. The transgenic plants exhibited an altered phenotype, although it varied between ...
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Pflp gene transformation in pomegranate (Punica granatum L.) resistance to bacterial blight disease (Xanthomonas axonopodis pv. punicae)
... transgene, Agrobacterium Ti plasmid-based vectors continue to offer the best system for plant transformation (Veluthambi et ...through Agrobacterium with both disarmed and wild strains have been published ...
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Identification of Strains ofAlcaligenes and Agrobacterium by a Polyphasic Approach
... The dendrogram obtained from EcoRI patterns revealed four ribogroups (Fig. 2). Ribogroup I included all the strains of A. xylosoxidans subsp. xylosoxidans. It was homogeneous and composed mostly of strains with a pattern ...
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Efficient method for Agrobacterium-mediated genetic transformation of tobacco nodal segments
... ABSTRACT. Plant transformation is a widely used procedure for obtaining transgenic plants and to develop plant models to understand gene function. Plant models such as Nicotiana tabacum are widely used for understanding ...
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REGENERATION AND AGROBACTERIUM-MEDIATED TRANSFORMATION OF WATERMELON
... supplimented with agar and phytagel individually or/and together were tested. Agrobacterium-mediated transformation system: The explants of watermelon cv Giza 1 and Giza 21 were co-cultivated with A. tumefaciens ...
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