Airway epithelia
Incorporation of Adeno-Associated Virus in a Calcium Phosphate Coprecipitate Improves Gene Transfer to Airway Epithelia In Vitro and In Vivo
... Adeno-associated viruses (AAVs) show promise as gene transfer vectors because they are capable of long-term gene expression in vivo, and since they do not encode viral genes, they do not elicit a cell-mediated immune ...
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Adenovirus-mediated gene transfer to ciliated airway epithelia requires prolonged incubation time.
... as airway epithelia differentiate and develop a ciliated surface, they are less readily infected by adenovirus ...ciliated airway epithelia in mice are resistant to adenovirus-mediated gene ...
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Human Coronavirus 229E Infects Polarized Airway Epithelia from the Apical Surface
... human airway epithelia. Differentiated hu- man airway epithelia were fixed for 20 min in 4% paraformaldehyde and rinsed twice in phosphate-buffered saline (PBS) for 10 min each ...
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The SPLUNC1-βENaC complex prevents Burkholderia cenocepacia invasion in normal airway epithelia
... the airway surface liquid of CF HBECs and was also ineffective at reducing cellu- lar invasion into CF cultures ...CF airway surface liquid leads to a double hit in that it (i) directly reduces SPLUNC1’s ...
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A Chimeric Type 2 Adenovirus Vector with a Type 17 Fiber Enhances Gene Transfer to Human Airway Epithelia
... of airway epithelia (13, ...human airway epithelia by ...on airway epithelia, may yield new insight into the apparent effects of length versus knob sequence on ...differentiated ...
7
Targeting the urokinase plasminogen activator receptor enhances gene transfer to human airway epithelia
... For a target we considered GPI-linked receptors because they reside on the apical surface of polarized epithelia (28). Although GPI-linked proteins do not have trans- membrane or cytoplasmic domains, when the ...
9
17β Estradiol inhibits Ca2+ dependent homeostasis of airway surface liquid volume in human cystic fibrosis airway epithelia
... disadvantage are unknown. Despite the lack of CFTR, CF airways retain a limited capability to regulate ASL volume, as breathing-induced ATP release activates salvage purinergic pathways that raise intracellular Ca 2+ ...
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Lentivirus Vectors Pseudotyped with Filoviral Envelope Glycoproteins Transduce Airway Epithelia from the Apical Surface Independently of Folate Receptor Alpha
... We investigated FR␣ expression in primary cultures of well- differentiated human airway epithelia. To determine the po- larity of expression, we immunostained the primary cultures with an FR␣-specific ...
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Lack of high affinity fiber receptor activity explains the resistance of ciliated airway epithelia to adenovirus infection
... of airway epithelia in a manner analogous to what has been done with retroviruses ...the airway epithelia can be targeted specifically by delivery of an aerosol, strategies that involve ...
7
Severe Acute Respiratory Syndrome Coronavirus Infection of Human Ciliated Airway Epithelia: Role of Ciliated Cells in Viral Spread in the Conducting Airways of the Lungs
... the airway epithelia of the mouse, hamster, and ferret models (34, 44, 45, 56); however, specific infection of ciliated cells was not described for these ...
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cAMP stimulates bicarbonate secretion across normal, but not cystic fibrosis airway epithelia
... human airway epithelial cells bathed in a HCO3-/CO2-buffered, Cl(-)- free ...across airway epithelium, and suggest that HCO3- leaves the cell across the apical membrane via conductive ...across ...
7
Vaccinia Virus Entry, Exit, and Interaction with Differentiated Human Airway Epithelia
... viral concentration gradient, and the tight junction protein ZO-1 showed a normal expression pattern. In addition, airway epithelia were spared from extensive lysis, and instead, cell number and epithelial ...
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Chronic β2AR stimulation limits CFTR activation in human airway epithelia
... in airway cells has not been ...in airway epithelia) despite a recommendation of “insufficient evidence to support chronic use” from the CF Foundation (23, ...daily airway clearance regimens, ...
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Defective fluid transport by cystic fibrosis airway epithelia
... CF airway epithelia without holding transepithelial voltage and ion concentration gradients at ...normal epithelia, cAMP agonists plus amiloride stimulated NaCl and fluid ...CF epithelia, cAMP ...
6
Selective response of human airway epithelia to luminal but not serosal solution hypertonicity Possible role for proximal airway epithelia as an osmolality transducer
... hypertonicity slows the rate of Na+ absorption but does not induce Cl- secretion; and (d) cell volume loss increases the resistance of the paracellular path. We speculate that these properties configure human nasal ...
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Fluid and electrolyte transport by cultured human airway epithelia
... accompanied Na+. The combination of cAMP agonists and mucosal amiloride stimulated the secretion of NaCl-rich fluid. But surprisingly, the response to cAMP agonists in the absence of amiloride showed substantial ...
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Toxicological and analytical assessment of e cigarette refill components on airway epithelia
... Thermo Fisher Scientific, Altrincham, Greater Manchester, UK: 0.25% Trypsin- EDTA (GIBCO), sterile phosphate buffered saline, acetonitrile optimal LCMS grade (ACN), ammonium acetate analytical grade, formic acid optimal ...
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Transduction of ferret airway epithelia using a pre-treatment and lentiviral gene vector
... because airway ciliated cells are a primary target for a CF gene therapy, with the aim to restore the ASL depth and allow normal mucocilli- ary clearance to be driven by properly functioning ciliated ...
7
nihms922052.pdf
... A high-fidelity computational fluid dynamics (CFD) method can accurately predict mechanical forces and water evaporative fluxes in the human airways and, consequently, could be coupled with a biophysical cell model of ...
29
Genetic Regulation of Zfp30, CXCL1, and Neutrophilic Inflammation in Murine Lung
... response, but the fact that Zfp30 expression was primarily localized to airway epithelia (not an immune cell) suggests the latter. Innate immune responses are known to prime ac- quired immune responses ...
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