alamar blue
Application of a high throughput Alamar blue biofilm susceptibility assay to Staphylococcus aureus biofilms
... Alamar blue has been used extensively in mammalian cell culture cytotoxicity assays and planktonic bacterial and fungal susceptibility assays, and is a simple, one-step pro- cedure, in which metabolic ...
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Rapid, Low Technology MIC Determination with Clinical Mycobacterium tuberculosis Isolates by Using the Microplate Alamar Blue Assay
... 103 Alamar Blue (Accumed International, Westlake, Ohio) reagent and 10% Tween 80 was added to well ...remained blue, the reagent mixture would be added to another control well and the result would be ...
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ANTI TUBERCULOSIS ACTIVITY OF MURRYA KOENIGII USING TH MICRO PLATE ALAMAR BLUE ASSAY
... The present study describes the Anti-tuberculosis (anti-TB) activity of oil obtained by hydro-distilation from the leaves of Murraya koengii by Micro-plate Alamar blue assay (MABA). The oil of Murraya ...
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Impact of riboflavin UVA photodynamic inactivation (PDI) (collagen crosslinking technique) on viability, cell cycle phase, apoptosis and proliferation of human corneal endothelial cells
... the Alamar blue assay as follows: HCECs were seeded in 24-well cell culture plates at a concentration of ...riboflavin-UVA-PDI, Alamar blue solution was diluted with culture medium for a final ...
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DESIGN, SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF NOVEL ANTI TUBERCULAR AGENTS TARGETING GLUTAMINE SYNTHETASE 1 AND CYCLOPROPANEMYCOLIC ACID SYNTHASE 2
... Using Alamar Blue Dye: The anti- mycobacterial activity of the synthesised compounds was assessed against ...using Alamar Blue micro plate assay ...
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The BET bromodomain inhibitor exerts the most potent synergistic anticancer effects with quinone-containing compounds and anti- microtubule drugs
... using Alamar blue assays showed that 273 compounds reduced the number of viable BE(2)-C cells by ≥ 90% on their own, and also reduced the number of viable BE(2)-C cells by ≥ 80% when combined with JQ1 ...
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Evaluating the anti Mycobacterium tuberculosis activity of Alpinia galanga (L ) Willd axenically under reducing oxygen conditions and in intracellular assays
... There has been no anti-TB drug introduced in the past 30 years and the rapid acquisition of drug resistance to the existing drugs necessitates development of new, ef- fective and affordable anti-TB drugs [24]. ...
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Biocompatibility of Various Kinds of Polymer Microspheres Estimated from Their Effect on Gap Junctional Intercellular Communication of Fibroblasts
... crospheres for 1 day was almost similar in all cases, inconsis- tent with their inhibitory activities on the GJIC function. In addition, a change in the phosphorylated status of the con- nexin was hardly indicated after ...
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Evaluation of Polyherbal Anticancer Tablets: A Review
... Caspase-9, alamar blue, LDH assay, XTT assay, sulforhodamine-B assay, MTT assay, DNA fragmentation assay, neutral red uptake cytotoxic assay, tryphan blue ...
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Cytosolic Ca2+ shifts as early markers of cytotoxicity
... the Alamar Blue viability assay is based on mitochondrial hydrolase activity that is generally affected by many different drugs as well as radiation ...[6-8]. Blue resazurin is metabolized into pink ...
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ZINC OXIDE NANOPARTICLES AS POTENTIAL NOVEL ANTICANCER THERAPIES
... Cells were incubated for 4 hours with Alamar Blue each day (added at 10% total well volume each day) and fluorescence read using a spectrophotometer. A 4-day proliferation curve was g[r] ...
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Fatty acid profile and proliferation of bovine blood mononuclear cells after conjugated linoleic acid supplementation
... AB: Alamar blue; ANOVA: analysis of variance; CLA: conjugated linoleic acid; CON: control group; ConA: concanavalin A; DM: dry matter; FA: fatty acid; NEFA: non esterified fatty acids; O[r] ...
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A REVIEW ON ANTIPROLIFERATIVE ACTIVITY OF PLANT EXTRACTS AGAINST BREAST CANCER CELL LINES
... (Alamar blue assay); WST (2-(4-iodophenyl)-3-(4- nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt); Luminogenic ATP (Adenosine triphosphate); RTCA-MP (real-time cell impedance-based cell ...
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Evaluation of Alamar colorimetric broth microdilution susceptibility testing method for staphylococci and enterococci
... The Alamar colorimetric antimicrobial susceptibility testing method uses an oxidation-reduction color indicator (Alamar Blue) to detect bacterial growth in the wells of microtiter ...the ...
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Development of an in vitro drug screening assay using Schistosoma haematobium schistosomula
... of Alamar Blue W and a redox indicator of en- zyme activity, using selected compounds of known antischistosomal activity such as praziquantel, meflo- quine, artesunate, metrifonate, and oxamniquine was ...
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Investigating NF-kappa B signaling in lung fibroblasts in 2D and 3D culture systems
... Recombinant human TNF-α was purchased from Gibco (Life Technologies, UK) and Ready-To-Glow TM NF-κB secreted luciferase reporter system (Cat No-631743) was obtained from Clontech laboratories (Palo Alto, CA). The ...
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Using a Non-Image-Based Medium-Throughput Assay for Screening Compounds Targeting N-myristoylation in Intracellular Leishmania Amastigotes
... an Alamar blue-based detection method, parasite survival following drug treatment of infected macrophages is monitored after macrophage lysis and transformation of freed amastigotes into replicative ...
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DESIGN, SYNTHESIS AND EVALUATION OF ANTITUBERCULAR ACTIVITY OF AMINO AZETIDINONES FROM ISONIAZID
... ABSTRACT: Azetidin-2-one analogues are reported to exhibit various pharmacological activities like cholesterol absorption inhibitory activity, human tryptase, thrombin and chymase inhibitory activity, vasopressin V1a ...
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ANTI-TUBERCULAR ACTIVITY OF THE RHIZOME OF CURCUMA PSEUDOMONTANA J. GRAHAM
... Tuberculosis is a highly infectious disease, with approximately one third of the world’s population including 26 % from India estimated to be infected it. However, this problem has become serious as Mycobacterium ...
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Anti-cancer Activity and Gene Expression Responses to Methanol Extract of Gladiolus segetum in THP-1 Human Monocytic Leukemia Cells
... (Alamar Blue, WST-1) and the trypan blue exclusion test in Human Leukemia Monocytes THP-1 and Using reverse transcription-polymerase chain reaction ...using Alamar Blue, WST-1 and ...
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