Alcian blue stain
Detection and Initial Characterization of Novel Capsular Polysaccharide among Diverse Campylobacter jejuniStrains Using Alcian Blue Dye
... CPS was found to be relatively tightly bound to the bacteria and could not be released by vortexing in saline at room tem- perature (Fig. 2B, lane 1). However, after incubation of bac- terial suspension at 50°C in ...
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Advancing osteochondral tissue engineering: bone morphogenetic protein, transforming growth factor, and fibroblast growth factor signaling drive ordered differentiation of periosteal cells resulting in stable cartilage and bone formation in vivo.
... number was similar to those in all the experimental con- ditions, but significantly lower than that of normal knees (P < 0.001) (Fig. 4). Histological characterization showed heterogeneous osteochondral regeneration ...
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Original Article Histologic features of sublingual gland herniation through the mylohyoid muscle
... and Alcian Blue (pH ...and Alcian blue staining showed that both acidic and neutral mucinous acini of junctional and herni- ated parts were ...
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The Effect of Growth Differentiation Factor-5, 6, 7 in Chondrogenic Cell Differentiation of ATDC-5
... by alcian blue ...week, alcian blue staining was performed as described previously with minor modifications ...0.5% Alcian Blue Stain Solution ...of alcian ...
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3D representation of Wnt and Frizzled gene expression patterns in the mouse embryo at embryonic day 11 5 (Ts 19)
... with Alcian blue staining, Fzd1, where it is most intense in patches in the anterior shaft, Fzd4, with three discrete patches in limb mesenchyme- in the posterior distal region of the progress zone and ...
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Immunohistochemical investigations and introduction of new therapeutic strategies in scleromyxoedema: Case report
... and Alcian blue staining revealed marked mucinous deposi- tion within the upper and mid dermis beside an increased appearance of fibroblasts, collagen bundles and a discrete inflammatory ...
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The Histological Evaluation of Sea Cucumber Meal as a Potential Ingredient in Rainbow Trout Diet
... Sea cucumber meal is proposed as ingredient for rainbow trout diet using an histological approach. Three isoproteic (crude protein 39.5%) and isoenergetic (18 MJ/kg DM) diets were formulated with increasing level of sea ...
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Adenomatous Polyposis Coli-mediated Control of β-catenin is Essential for Both Chondrogenic and Osteogenic Differentiation of Skeletal Precursors
... an Alcian blue-stained matrix, and expressed the nascent chondrocyte markers Sox9 and ...not stain positively for β-catenin, displayed an intensely Alcian blue-stained matrix and ...
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Histochemical structure and immunolocalisation of the hyaluronan system in the dromedary oviduct
... Alcian blue staining along the luminal surface of both ciliated and non-ciliated epithelial cells (LE).. Staining for HAS2 was higher in the primary epithelial folds of the ampulla compa[r] ...
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Two Separated Protocols with the Most Important Comments for Skeletal Staining in Embryonic and Adulthood Period in Laboratory Animals
... ethanol, alcian blue staining, dehydration by ethanol, alizarin red staining, washing by potassium hydroxide and glycerol, storage in pure glycerol were the stages of embryos and newborns skeletal ...
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DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013
... Both Alcian Blue staining and quantitative analysis results showed that gly- cosaminoglycan formation was increased in LINC01013 knocked down SCAPs compared to control cells ...the Alcian Blue ...
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Investigation of stemness and multipotency of equine adipose-derived mesenchymal stem cells (ASCs) from different fat sources in comparison with lipoma
... The evaluation of the chondrogenic potency of ASCs re- vealed a higher capacity of RP and SC fat-derived cells to- wards cartilage formation as shown by Alcian blue staining and upregula[r] ...
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A Review Study: Using Stem Cells in Cartilage Regeneration and Tissue Engineering
... (F) Alcian-blue-stained histology of engineered cartilage construct generated using human mesenchymal stem cells, showing abundant cartilaginous matrix surrounding large chondrocytes.. [r] ...
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Effects of pithecellobium jiringa ethanol extract against ethanol induced gastric mucosal injuries in Sprague Dawley rats
... Figure 3. Histological study of the absolute ethanol-induced gastric mucosal damage in rats. (a) Rats pre-treated with CMC (normal control). No disturbance of gastric mucosa. (b) Rats pre-treated with CMC (ulcer ...
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MORPHO-ANATOMICAL, HISTOCHEMICAL, PHYSICO-CHEMICAL AND THIN LAYER CHROMATOGRAPHY ANALYSIS ON Annona muricata L. SEEDS
... and blue colour pattern of the cellulose orientation in the cell wall of endosperm ...brilliant blue which shows blue colour for protein ...the blue colouration in the endosperm ...showing ...
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Global proteomic analysis of plasma from mice infected with Plasmodium berghei ANKA using two dimensional gel electrophoresis and matrix assisted laser desorption ionization time of flight mass spectrometry
... Analytical gels were silver stained using a protocol described by Blum et al [9] with modifications described by Rabilloud et al [10]. Preparative gels were stained using colloidal Coomassie Brilliant Blue G-250 ...
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Pleural epithelioid angiosarcoma with lymphatic differentiation arisen after radiometabolic therapy for thyroid carcinoma: immunohistochemical findings and review of the literature
... Background: Pleural angiosarcoma is a rare tumor that causes diffuse pleural thickening and effusion, mimicking mesothelioma. Immunohistochemistry is needed to highlight endothelial differentiation. We describe the first ...
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Comparative Study of Toluidine Blue Special Stain and Immunohistochemistry in Hirschsprung Disease
... Toluidine blue stain : (also known as tolonium chloride) it is a synthetic, acidophilic metachromatic dye which has an high affinity for nucleic acids, and it binds to nuclear material with a high DNA and ...
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Synovial fluid level of aggrecan ARGS fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan levels: a cross sectional study
... The molar proportion of aggrecan fragments in synovial fluid SF; measured by Alcian blue precipitation detected as ARGS neoepitope fragments measured by ARGS ELISA in the study groups he[r] ...
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MNN5 Encodes an Iron-Regulated α-1,2-Mannosyltransferase Important for Protein Glycosylation, Cell Wall Integrity, Morphogenesis, and Virulence in Candida albicans
... cerevisiae Mnn5 and Mnn2, but our complementation test showed that Ca MNN5 restored Alcian blue binding to the cell surface of mnn5 ⌬ but not mnn2 ⌬ mutant cells, suggesting that Ca MNN5[r] ...
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