alkaline comet assay
Studies on electron beam induced DNA damage and repair kinetics in lymphocytes by alkaline comet assay
... by alkaline comet assay INTRODUCTION Exposure to ionizing radiation is known to induce oxidative stress followed by damage to critical biomolecules like lipids, proteins and DNA through radiolysis of ...
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ALKALINE COMET ASSAY AND MICRONUCLEUS TEST PARAMETERS IN CHILDREN EXPOSED TO DIAGNOSTIC X-RAY EXAMINATION
... the comet assay and micronucleus test parameters before and after X-ray diagnostics are summarized in the Figure ...as alkaline comet assay parameter and the total number of micronuclei ...
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IN VITRO AND IN VIVO GENOTOXICITY ASSESSMENT OF TOTAL ALKALOIDS OF AGERATUM CONYZOIDES L LEAVES (ASTERACEAE) BY ALKALINE COMET ASSAY
... Some pyrrolizidine alkaloids have been identified in A. conyzoides 7-10 and few reports have shown the genotoxicity of lycopsamine and echinatine, two pyrrolizidine alkaloids present in this plant 7, 15 . Because of the ...
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Radiosensitivity and Repair Kinetics of Gamma-Irradiated Leukocytes from Sporadic Prostate Cancer Patients and Healthy Individuals Assessed by Alkaline Comet Assay
... Study of repair kinetics. In order to investigate the rejoining of DNA breaks, the changes in the residual DNA damage in 5 prostate cancer patients and 5 healthy volunteers at 0, 15, 30, 60, 120, 180 min, and 24 h after ...
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Genotoxic carbofuran effect evaluation in mice by alkaline comet assay ussage
... alkali comet assay usage on four organs´ cells of mice strain swiss albino in conditions in ...two comet assay parameters: tail length and tail ...
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Genotoxic effects of boric acid and borax in zebrafish, Danio rerio using alkaline comet assay
... Keywords: boric acid, borax, genotoxicity, Comet assay, Danio rerio INTRODUCTION Boron is naturally present everywhere on the earth, including in rocks, soils, and natu- ral waters. Especially, ...
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A Comprehensive Review on Clinical Applications of Comet Assay
... damage, alkaline comet assay is proven to be a relatively simple and versatile tool in the assessment of DNA damage and also in determining the efficacy of DNA repair ...of comet assay ...
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DNA repair as a human biomonitoring tool: comet assay approaches.
... repair assay, or extracts with a high repair activity for the in vitro repair ...the alkaline comet assay have been developed in order to 443 increase the ...throughput comet ...
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DNA DAMAGE ANALYSIS IN MAMMALIAN CELLS BY SMA-DMSO COMPLEX USING COMET ASSAY
... 15. Rydberg, B.Radiation-induced heat-labile sites that convert into DNA double-strand breaks. Radiat. Res. 153, 805−812,2000. 16. Collins, A.R.The comet assay for DNA damage and repair: principles, ...
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Evaluation of gamma radiation-induced DNA damage in Aedes aegypti using the comet assay
... the alkaline comet assay is a promising technique to assess the genotoxic potential of gamma radiation in ...this assay may be used to explain the connection between the stress that is induced ...
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Evaluation of DNA Damage in the Buccal Cells of patients with Oral Submucous Fibrosis using Comet Assay
... using alkaline comet assay. It was found that comet assay parameters were significantly higher in the group wearing metal appliances when compared to the control ...
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Assessment of DNA Damage in Pulmonary Tuberculosis Patients by Single Cell Gel Electrophoresis / Comet Assay
... Semra Sardasa et al (2000), study provide evidence of oxidative damage in diabetic patients when evaluated by alkaline comet assay. Difference could be detected in IDDM and NIDDM patients groups in ...
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DNA damage and cell death assessment in patients with severe multiple trauma using comet assay
... using comet FLARE assay no differences in 8-oxoG levels were observed, despite a markedly reduced ...repair. Alkaline comet assay detects strand breaks, alkali-labile sites, as well as ...
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Evaluation of DNA Damage by Methyl Methane Sulfonate in Allium cepa Root Cells by Comet Assay
... mild alkaline comet assay (Single Cell Gel ...growth assay, a significant increase in comet tail length, %Tail DNA, Tail Moment (TM) and Olive Tail Moment (OTM) was noticed in root cell ...
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Assessment of DNA damage in pulmonary tuberculosis patients by single cell gel electrophoresis / comet assay
... reliable comet measurements that would truly reflect the extent of DNA damage induced by the bacterial infectious disease, and was assessed by performing the alkaline comet assay on venous ...
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Optimization of Alkaline Single Cell Gel Electropohoresis (Comet Assay) Protocol for Cells from Oral Cavity
... the comet assay technique in mucosa buccal cells that is expressed as a higher proportion of USCN and a good response to oxidative ...the comet tail; iv) a seven-day period of incubation that ...
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INVESTIGATION OF CARCINOGENIC AND MUTAGENIC PROPERTY OF FOOD COLOR USING CAT FISH CLARIAS BATRACHUS BY USING ALKALINE SINGLE-CELL GEL ELECTROPHORESIS (COMET) ASSAY AND MICRONUCLEUS ASSAY
... breakage or DNA cross-links and incomplete excision repair even 7 . DNA lesions have been detected by inducing chemical mutagens using Comet assay. Fishes are generally used as toxicity indicators. Teleosts ...
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Genetic polymorphisms and the effect of cigarette smoking in the comet assay
... the comet assay (alkaline single cell gel electrophoresis) and conflicting results have been ...baseline comet assay effects obtained for nonsmokers and smokers in relation to selected ...
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Evaluation of the Genotoxicity of Chlorpyrifos in Common Indus Valley Toad, Bufo stomaticus Using Alkaline Single Cell Gel Electrophoresis (Comet) Assay
... Blood cells were collected at every 24 h interval and were subjected to the Alkaline Single-Cell Gel Electrophoresis assay. The observed DNA damage was concentration and time-dependent, and those levels of ...
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ab83369 Alkaline Phosphatase Assay kit (Colorimetric)
... GENERAL INFORMATION 8. TECHNICAL HINTS This kit is sold based on number of tests. A ‘test’ simply refers to a single assay well. The number of wells that contain sample, control or standard will vary by product. ...
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