Alkaline comet assay results
Genotoxic carbofuran effect evaluation in mice by alkaline comet assay ussage
... given results it can be concluded that carbofuran and Geocid ST 35 have genotoxic effect on all treated murine organs, but niether carbofuran nor its formulation have shown specificy of genotoxic effect on niether ...
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Studies on electron beam induced DNA damage and repair kinetics in lymphocytes by alkaline comet assay
... using alkaline comet ...a comet parameter. Results and Conclusion: The study reveals that, induc on of DNA damage by 8 MeV pulsed electron beam is near linear with a negligible nega ve quadra ...
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ALKALINE COMET ASSAY AND MICRONUCLEUS TEST PARAMETERS IN CHILDREN EXPOSED TO DIAGNOSTIC X-RAY EXAMINATION
... RESULTS The X-radiation exposures measured on the back of 18 patients varied from 0.13 mSv to 0.29 mSv, whereas X-radiation exposures measured on the sternum ranged from 0.01 mSv to 0.04 mSv. Thyroid exposures ...
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Optimization of Alkaline Single Cell Gel Electropohoresis (Comet Assay) Protocol for Cells from Oral Cavity
... the comet assay technique in mucosa buccal cells that is expressed as a higher proportion of USCN and a good response to oxidative ...agarose results in a more uniform and efficient digestion; iii) ...
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IN VITRO AND IN VIVO GENOTOXICITY ASSESSMENT OF TOTAL ALKALOIDS OF AGERATUM CONYZOIDES L LEAVES (ASTERACEAE) BY ALKALINE COMET ASSAY
... and antilithic 15-16 . In Togo, A. conyzoides is reported to treat fever, measles and snake bites 13 . In addition to its popular use for skin diseases, wound healing, diarrhoea, pain associated with navel in children, ...
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INVESTIGATION OF CARCINOGENIC AND MUTAGENIC PROPERTY OF FOOD COLOR USING CAT FISH CLARIAS BATRACHUS BY USING ALKALINE SINGLE-CELL GEL ELECTROPHORESIS (COMET) ASSAY AND MICRONUCLEUS ASSAY
... found results were coherent with the studies conducted with redbreast sunfish from toxic loaded waters which had increased level of single breakage of DNA when tested with SCGE 10 ...
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Genetic polymorphisms and the effect of cigarette smoking in the comet assay
... the comet assay (alkaline single cell gel electrophoresis) and conflicting results have been ...discrepant results. We therefore evaluated our baseline comet assay effects ...
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Radiosensitivity and Repair Kinetics of Gamma-Irradiated Leukocytes from Sporadic Prostate Cancer Patients and Healthy Individuals Assessed by Alkaline Comet Assay
... RESULTS Initial radiosensitivity of prostate cancer patients and healthy controls. The ratio of the DNA intensity in the tail to that in the head region of the comet (Tail DNA/Head DNA) was used as a ...
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Evaluation of the Genotoxicity of Chlorpyrifos in Common Indus Valley Toad, Bufo stomaticus Using Alkaline Single Cell Gel Electrophoresis (Comet) Assay
... 3. Results Physico-chemical characteristics of the water used in the experiments recorded, as pH: 8.2 ± 0.1; temperature: 26˚C ± 1˚C; dissolved oxygen: 8.6 ± 0.2; electrical conductivity: 2.65 - 2.75 mS/m; total ...
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Fluorescence in situ hybridization in combination with the comet assay and micronucleus test in genetic toxicology
... upon alkaline denaturation and electrophoresis. Comet-FISH with a probe for the p53 gene was applied to cells that had been damaged by ionizing ...The results obtained favored the idea that both ...
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Evaluation of DNA Damage in the Buccal Cells of patients with Oral Submucous Fibrosis using Comet Assay
... Some results indicate that alkaline single-cell gel electrophoresis, using buccal epithelial cells, could be a good biomarker of early effects, and can be utilized for human ...
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Assessment of DNA damage in pulmonary tuberculosis patients by single cell gel electrophoresis / comet assay
... reliable comet measurements that would truly reflect the extent of DNA damage induced by the bacterial infectious disease, and was assessed by performing the alkaline comet assay on venous ...
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Genotoxic effects of boric acid and borax in zebrafish, Danio rerio using alkaline comet assay
... Keywords: boric acid, borax, genotoxicity, Comet assay, Danio rerio INTRODUCTION Boron is naturally present everywhere on the earth, including in rocks, soils, and natu- ral waters. Especially, ...
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The effect of different methods and image analyzers on the results of the in vivo comet assay
... vitro comet assay, and an increase in % tail DNA values in re- sponse to decreased agarose concentrations and ex- tended unwinding and electrophoresis times has been reported [16, ...these results, ...
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A Comprehensive Review on Clinical Applications of Comet Assay
... damage, alkaline comet assay is proven to be a relatively simple and versatile tool in the assessment of DNA damage and also in determining the efficacy of DNA repair ...of comet assay ...
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DNA repair as a human biomonitoring tool: comet assay approaches.
... The comet assay offers the opportunity to measure both DNA damage and ...47 comet assay based methods are available to measure DNA repair activity, but some 48 requirements should be met for ...
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ab83369 Alkaline Phosphatase Assay kit (Colorimetric)
... GENERAL INFORMATION 8. TECHNICAL HINTS This kit is sold based on number of tests. A ‘test’ simply refers to a single assay well. The number of wells that contain sample, control or standard will vary by product. ...
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Comet assay as a sensitive technique in occupational health studies; A literature review
... ‘comet assay’ and ‘DNA damage’, and in various combinations with occupational ...employing comet assay in different industrial milieus (N=49) were originally published from 2000 to ...
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Comparison of comet assay parameters for estimation of genotoxicity by sum of ranking differences
... the comet assay should also be related to the ability of the tissue to take up and metabolize ...the comet assay is the blood, because it is easy to collect and there is no need for cellular ...
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Komet 7. Acquisition and Analysis Software for the Comet Assay - Research and GLP
... all comet images, parameters and audit trails Proven performance - reliable data analysis with backward compatibility Integrated creation of summary statistics - optimizes data workflow and reporting Facilitates ...
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