alveolar epithelial cells type 2
Wnt signaling regulates trans-differentiation of stem cell like type 2 alveolar epithelial cells to type 1 epithelial cells
... transglutaminase 2 (TGM2), integ- rin alpha V (ITGAV), epithelial membrane protein 2 (EMP2), cytochrome P450 family 4, subfamily B, polypep- tide 1 (CYP4B1) and ankyrin repeat domain 1 (ANKRD1) as ...
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Fraction of MHCII and EpCAM expression characterizes distal lung epithelial cells for alveolar type 2 cell isolation
... file 2: Table ...dead cells, 7-amino actinomycin D (7-AAD, BD Biosciences) was added to the samples before ...single cells using a FAC- SAria III Cell Sorter with FACSDiva ...Sorted cells were ...
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Nuclear factor-kappa B influences early phase of compensatory lung growth after pneumonectomy in mice
... in type 2 epithelial cells ...small alveolar space including alveoli and alveo- lar ...disturbed alveolar enlargement after pneumonectomy that is considered to be the possible ...
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Human alveolar epithelial cells type II are capable of TGFβ-dependent epithelial-mesenchymal-transition and collagen-synthesis
... these cells and seem to interfere with a TGFβ-mediated effect in ...the Type 2 EMT of ...primary cells might acquire the Type 2 EMT state only transiently and reverse their ...
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Hermansky-Pudlak syndrome type 2 manifests with fibrosing lung disease early in childhood
... hyperplastic type II pneumocytes (G: HE, x400) as subtle but characteristic features of HPS ...in alveolar epithelial cells type II (AECII) in HPS2 patient ...
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Proliferative regulation of alveolar epithelial type 2 progenitor cells by human Scnn1d gene
... and epithelial cells ...classic epithelial αβγ -ENaC channels are a major regulator for maintaining electrolytes and fluid homeostasis in the lung as well as the kidney ...numerous cells and ...
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Mac-1 deficiency induces respiratory failure by affecting type I alveolar epithelial cells.
... Lung tissues were fixed in formalin overnight, then dehydrated, cleared, and embedded as follows: 70% ethanol for 3 h, 80% ethanol for 3 h, 95% ethanol for 2 h, 100% ethanol for 1.5 h (twice), xylene for 0.5 h ...
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Type 2 alveolar cells are stem cells in adult lung
... the epithelial cells within spheres were lineage ...eral cells were cuboidal, expressed SFTPC (but not SCGB1A1), and proliferat- ed as judged by Ki67 stain- ing (Supplemental Figure ...Interior ...
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Alert cell strategy in SIRS-induced vasculitis: sepsis and endothelial cells
... various epithelial cells and vascular endothelial ...dendritic cells, and the overproduction of inflammatory ...some epithelial cells in major organs express inflammatory receptors on ...
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Overexpression of cathepsin K in mice decreases collagen deposition and lung resistance in response to bleomycin-induced pulmonary fibrosis
... < 2 yr from time of diagnosis ...by alveolar epithelial cell injury and hyperplasia, inflammatory cell accumulation, fibrob- last hyperplasia, formation of honeycomb cysts and exu- berant ...
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Culture of human alveolar epithelial type II cells by sprouting
... AT2 cells or fibro- blasts was performed as previously described [15]: cells were fixed with 4% formalin (10 min), and permeabilized with methanol/acetic acid (3:1, ice-cold, 10 ...min). Cells were ...
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Alveolar Type II Epithelial Cells Contribute to the Anti Influenza A Virus Response in the Lung by Integrating Pathogen and Microenvironment Derived Signals
... of 2 or more over controls were considered indicative of up- or ...⫽ 2 individual samples for each condition; 5 mice per sample) was subjected to microarray ...ⱖ⫾ 2 (threshold represented by the ...
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Mechanisms of lung fibrosis induced by carbon nanotubes: towards an Adverse Outcome Pathway (AOP)
... adenocarcinoma alveolar epithelial cell line; ATII: primary human alveolar type-II epithelial cells; BALB-3 T3: mouse embryonic fibroblast cell line; BEAS-2B: human bronchial ...
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Fgf10 positive cells represent a progenitor cell population during lung development and postnatally
... Fgf10-expressing cells are progenitors for parabronchial smooth muscle cells (PBSMCs) in the distal lung during early embryonic development (Mailleux et ...mesenchymal cells, initially located near ...
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GABA receptor ameliorates ventilator induced lung injury in rats by improving alveolar fluid clearance
... The conditions used to induce lung injury vary among investigators and laboratories. For example, tidal volumes range from 20 to 40 ml/kg BW, and ventilation times, from 40 minutes to a few hours. Sometimes, ven- ...
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Exosome miR-371b-5p promotes proliferation of lung alveolar progenitor type II cells by using PTEN to orchestrate the PI3K/Akt signaling
... expressing cells should express NEO R ...SPC-expressing cells from the hESC-derived cultures using G418 selection strategy ...expressing cells also expressed other surfactant proteins and lamella ...
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Enhanced non vitreous cryopreservation of immortalized and primary cells by ice growth inhibiting polymers
... proliferation. This is typically not possible with primary cells in vitro, necessitating donors, which are a limited resource. Analysis of cryopreservation has identified that ice crystal growth ...
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Alveolar type II epithelial cells produce interleukin 6 in vitro and in vivo Regulation by alveolar macrophage secretory products
... pg/ml/10(6) cells, n = 8, P = 0.001). Rat alveolar macrophage conditioned media (CM) increased IL-6 secretion by rat ATII cells through the effect of IL-1 and ...A549 cells was induced by IL-1 ...
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Conditional overexpression of TGFβ1 promotes pulmonary inflammation, apoptosis and mortality via TGFβR2 in the developing mouse lung
... Many studies have demonstrated that TGFβ1 is a critical regulator of HALI [10,11]. Hyperoxia mediated TGFβ1 sets into flow a deluge of inflammation [12,13]. However, the mechanisms responsible for the TGFβ1-induced ...
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Examining the role of ABC lipid transporters in pulmonary lipid homeostasis and inflammation
... ATII cells from free cholesterol-induced cyto- toxicity by exporting free ...cholesterol. Cells transfected with a non-functional mutant of ABCA3, ABCA3- Q215K, displayed excessive accumulation of ...
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