Anchorage-independent growth
The first hydrophobic region of the HPV16 E5 protein determines protein cellular location and facilitates anchorage independent growth
... promotes anchorage-independent growth in soft agar ...keratinocyte growth in soft agar as a read-out for the biological activity of the protein and the corresponding ...
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Activation of NF-κB by the RANKL/RANK system up-regulates snail and twist expressions and induces epithelial-to-mesenchymal transition in mammary tumor cell lines
... Receptor activator of NF-κB (RANK) and RANK ligand (RANKL) were originally shown to be essential for osteoclastogenesis, lymph node development, and forma- tion of lactating mammary glands during pregnancy. Re- cent ...
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Anchorage independent colony growth of pulmonary fibroblasts derived from fibrotic human lung tissue
... Fibroblast heterogeneity is known to exist in chronically inflamed tissue such as pulmonary fibrosis (IPF) and scleroderma. We have previously shown differences in proliferation rates in primary lines and cloned lines of ...
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Crk adaptor proteins act as key signaling integrators for breast tumorigenesis
... To investigate how mammary tumor outgrowth is altered by Crk knockdown, 1833TR cells were analyzed three days post-injection for proliferation and apoptosis. Relative to control cells, Crk knockdown significantly ...
15
MIAT inhibits proliferation of cervical cancer cells through regulating miR-150-5p
... the anchorage-independent growth capacity was greatly compromised by ectopic MIAT expression in comparison to the control coun- terparts ...and anchorage-independent growth in ...
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ETV4 transcription factor and MMP13 metalloprotease are interplaying actors of breast tumorigenesis
... To that end, we established the MMT-ETV4 + shMMP13 cell line, which expresses an MMP13-shRNA construct allowing for a significant reduction in MMP13 mRNA expression and subsequently a reduction in MMP13 metalloprotease ...
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Integrated time course omics analysis distinguishes immediate therapeutic response from acquired resistance
... - Anchorage- independent growth of cetuximab generation 10 (CTX-G10); Figure S3 - Heatmap and hierarchical clustering of gene expression values in 11 gen- erations of SCC25 cells treated with PBS as ...
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A small molecule inhibitor of atypical protein kinase C signaling inhibits pancreatic cancer cell transformed growth and invasion
... cell anchorage-independent growth and cellular invasion [2, ...transformed growth of pancreatic cancer ...cell growth in soft agar and invasion (Figure 1B and ...cell growth in ...
14
Down regulation of Sp1 suppresses cell proliferation, clonogenicity and the expressions of stem cell markers in nasopharyngeal carcinoma
... the growth of NPC ...clonogenicity, anchorage-independent growth and the stem-cell like phenotype of NPC cells through inducing the expression of p27 and p21, and through impairing the ...
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<p>Long Non-Coding RNA LEF1-AS1 Promotes Migration, Invasion and Metastasis of Colon Cancer Cells Through miR-30-5p/SOX9 Axis</p>
... and anchorage-independent growth in vitro and tumor xenograft growth and lung metastasis in ...invasion, anchorage-independent growth and tumor xenograft ...
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<p>ZNF433 positively regulates the beta-catenin/TCF pathway in prostate cancer and enhances the tumorigenicity of cancer cells</p>
... Notes: (A) Luciferase assay was performed to examine the effects of ZNF433 on the reporter activity of TOPflash. Cells were treated with or without the Wnt3a protein. (B) The effects of ZnF433 knockdown on the expression ...
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Small ubiquitin-like modifier 1 modification of pyruvate kinase M2 promotes aerobic glycolysis and cell proliferation in A549 human lung cancer cells
... the growth promotion effect was com- parable between wild-type and K336R mutant PKM2 cells in the absence of SUMO1 (P = ...the anchorage-independent growth of cancer cells compared with K336R ...
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Silencing of GM3 synthase suppresses lung metastasis of murine breast cancer cells
... and anchorage-independent growth of breast cancer cells described above, we next examined the effects of GM3S silencing on tumor formation and metastasis of breast cancer ...
12
Epstein-Barr Virus Contributes to the Malignant Phenotype and to Apoptosis Resistance in Burkitt’s Lymphoma Cell Line Akata
... cells, anchorage-independent growth in soft agar, tumorigenicity in SCID mice, and apoptosis resis- tance, we isolated EBNA-1-expressing clones by transfecting EBNA-1/oriP vector, which contains neoR ...
7
Mitochondrial clearance by the STK38 kinase supports oncogenic Ras-induced cell transformation
... resistance, anchorage-independent soft agar growth, and in vivo xenograft growth of Ras-transformed human ...and anchorage- independent growth of Ras-transformed human ...
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PPMP, a novel tubulin-depolymerizing agent against esophageal cancer in patient-derived tumor xenografts
... In the present study, we report the discovery of a novel microtubule-destabilizing compound referred to as PPMP. The result of ex vivo and in vitro tubulin polymerization assays clearly showed that PPMP markedly ...
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Fluctuation of simian virus 40 (SV40) super T-antigen expression in tumors induced by SV40-transformed mouse mammary epithelial cells.
... Anchorage-independent growth in vitro and tumorigenicity in nude mice of SV40-transformed mammary epithelial clonal cell lines Tumorigenicityb Anchorage independencea Cell line Presence [r] ...
5
Lipocalin 2 promotes lung metastasis of murine breast cancer cells
... and growth in the new location ...and anchorage- independent growth of 4T1 cells were not significant, our results from cell migration and invasion analysis indi- cated that cell mobility was ...
9
Cx43 Reduces Melanoma Growth Within a Keratinocyte Microenvironment and During Tumorigenesis in vivo
... downregulated in many cancer cell types rendering them GJIC-deficient (Cronier et al., 2009; Loewenstein and Kanno, 1966; Mesnil et al., 2005), and interestingly, their proliferation is often suppressed following ectopic ...
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Overexpression of SNHG12 regulates the viability and invasion of renal cell carcinoma cells through modulation of HIF1α
... Our previous results demonstrated that over-expres- sion of SNHG12 was prevalent in renal carcinoma, which intimately associated with poor outcomes. Then we attempted to experimentally validate the oncogenic properties ...
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