Annexin/PI
Cytotoxicity of quantum dots and graphene oxide to erythroid cells and macrophages
... cells from spleen. Since bone marrow and spleen are active sites of erythropoiesis in early course, we looked at the pro- portion of erythroid cells in spleen and bone marrow with FACS analysis. As shown in Figure 6D, ...
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<p>Synthesis, Characterization, Biomedical Application, Molecular Dynamic Simulation and Molecular Docking of Schiff Base Complex of Cu(II) Supported on Fe3O4/SiO2/APTS</p>
... complex through the use of (3-aminopropyl)triethoxysilane linker as chemotherapeutics to cancer cells. The nanoparticles were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray powder diffraction ...
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10<em>H</em>-3,6-Diazaphenothiazine induces G<sub>2</sub>/M phase cell cycle arrest and caspase-dependent apoptosis and inhibits cell invasion of A2780 ovarian carcinoma cells through the regulation of NF-κB and (BIRC6-XIAP) complexes
... an important role in the last stage of apoptosis as it acts as a marker to be engulfed by phagocytes. Therefore, PS can be used as a quantitative model to study apoptosis through counting the amount of Annexin ...
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The aqueous extract of <em>Brucea javanica</em> suppresses cell growth and alleviates tumorigenesis of human lung cancer cells by targeting mutated epidermal growth factor receptor
... ratio Annexin V/fluorescein isothiocyanate (FITC) (20 μ g/mL) and PI (50 μ g/mL) at room temperature for 30 minutes in ...of Annexin V/FITC (An) along with PI divides four population ...
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Involvement of Seladin 1 in goniothalamin induced apoptosis in urinary bladder cancer cells
... using Annexin V-FITC/PI staining, apoptosis and necrosis event was quantified by flow cy- tometry as shown in Figure ...of annexin V staining positive represent apoptotic event and PI staining ...
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Cytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells
... the annexin V-FITC/PI assay by flow cytometry and examined the ultrastructure of MDA-MB-231 cells by ...The annexin V-FITC/PI assay revealed an increase in early apoptotic cell populations ...
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SBF-1 inhibits contact hypersensitivity in mice through down-regulation of T-cell-mediated responses
... The structure of SBF-1 was shown in Fig. 1a. First we performed MTT assay, which is an effective method for testing mitochondrial impairment and correlates quite well with cell proliferation [15]. As shown in Fig. 1b, ...
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Influence of hypothermia on right atrial cardiomyocyte apoptosis in patients undergoing aortic valve replacement
... with annexin V in conjunction with vital dye such as PI allows to identify early apoptotic cells (annexin positive, PI negative) and non apoptotic non necrotic cells (annexin negative, ...
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Cell adhesion-mediated mitochondria transfer contributes to mesenchymal stem cell-induced chemoresistance on T cell acute lymphoblastic leukemia cells
... performed annexin V/PI flow cytometry analysis and a cell viability assay and found that Jurkat cells treated with cytochalasin D had an increased apoptosis rate ...and annexin V/PI results, ...
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The La protein counteracts cisplatin-induced cell death by stimulating protein synthesis of anti-apoptotic factor Bcl2
... Next we tested whether reduction of La protein expression will increase the sensitivity toward cisplatin in HNSCC cells. To reduce the La expression we applied two independent approaches: (1) siRNA- or shRNA-mediated ...
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Subchronic toxicity, immunoregulation and anti breast tumor effect of Nordamnacantal, an anthraquinone extracted from the stems of Morinda citrifolia L
... and Annexin V/PI assays on human MCF-7 and MDA-MB231 breast cancer ...and Annexin V results, nordamnacanthal managed to induce cell death in both MDA-MB231 and MCF-7 ...
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Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells
... and necrosis was identified in HuCC-T1 cells by ALA-PDT using FITC-annexin V and PI. The numbers of apoptotic and necrotic cells gradually increased on treatment with 1 mM ALA for 24 hours, indicating that ...
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Role of p38 Signaling Pathway in Pentagastrin Regulated Cell Proliferation of Colorectal Carcinoma Cell Line HT 29
... Pentagastrin was purchased from Shanghai Tian Yuan Biotechnology Co., Ltd. (Shanghai, China). Proglumide and the propidium iodide staining kit were purchased from Sigma (St. Louis, MO, United States). P38 rabbit ...
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miR-663 overexpression induced by endoplasmic reticulum stress modulates hepatocellular carcinoma cell apoptosis via transforming growth factor beta 1
... Notes: (A) TM co-incubated with mir-663 inhibitors in the combined effects on cell apoptosis. hepg2 cells were treated with the indicated concentrations of inhibitors (I663) and negative control at the indicated times ...
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Characterization of the non-glandular gastric region microbiota in Helicobacter suis-infected versus non-infected pigs identifies a potential role for Fusobacterium gastrosuis in gastric ulceration
... (green, Annexin-V-FITC negative, PI negative), early apoptotic (red, Annexin-V-FITC positive, PI negative), late apoptotic/necrotic (blue, Annexin-V-FITC posi- tive, PI positive) ...
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Protective functions of myricetin in LPS-induced cardiomyocytes H9c2 cells injury by regulation of MALAT1
... with Annexin V-FITC/ ...kit-8, Annexin V-FITC/PI Annexin V-fluorescein isothiocyanate/propidium iodide, qRT-PCR quantitative real-time PCR, ELISA enzyme-linked immune sorbent ...
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Shikonin induced necroptosis is enhanced by the inhibition of autophagy in non small cell lung cancer cells
... whether necroptosis is also induced in NCSCL cells. Flow cytometry with Annexin V and PI double staining is an effective method to quantitatively distinguish between necrosis and apoptosis. The flow ...
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Polysaccharopeptides derived from Coriolus versicolor potentiate the S-phase specific cytotoxicity of Camptothecin (CPT) on human leukemia HL-60 cells
... (PI-ve, annexin V-ve); R4 represents early apoptotic cells (PI-ve, annexin V+ve) and R2 rep- resents late apoptotic cells (PI+ve, annexin ...
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Up-Regulated Lipocalin-2 in Pulmonary Hypertension Involving in Pulmonary Artery SMC Resistance to Apoptosis
... of Annexin V/PI-positive cells using flow cytometry, and the detection of cleaved caspase-3 and caspase-3 activity, we observed that Lcn2 significantly inhibited HPASMC apoptosis induced by serum withdrawal ...
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Efficacy and safety of dendrimer nanoparticles with coexpression of tumor necrosis factor-α and herpes simplex virus thymidine kinase in gene radiotherapy of the human uveal melanoma OCM-1 cell line
... of annexin V/ propidium iodide double-dyeing apoptosis and caspase-3 fluorescence staining showed that this treatment method could turn transfected OCM-1 cells into apoptosis and necrosis by the effects of the ...
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