Anthrax protective antigen
Generation and Characterization of Human Monoclonal Antibodies Targeting Anthrax Protective Antigen following Vaccination with a Recombinant Protective Antigen Vaccine
... The anthrax protective antigen (PA) is the central component of the three-part anthrax toxin, and it is the primary immunogenic component in the approved AVA anthrax vaccine and the ...
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Rapid, Sensitive, and Specific Lateral-Flow Immunochromatographic Device To Measure Anti-Anthrax Protective Antigen Immunoglobulin G in Serum and Whole Blood
... anti-anthrax protective antigen (PA) immunoglobulin G (IgG) from vaccination with anthrax vaccine adsorbed (AVA) is protective against Bacillus anthracis ...19 anthrax AVA ...
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Induction of Neutralizing Antibody Responses to Anthrax Protective Antigen by Using Influenza Virus Vectors: Implications for Disparate Immune System Priming Pathways
... In vitro toxin neutralization. The ability of serum obtained in the experiments detailed in Fig. 2 and 3 to neutralize anthrax toxin in vitro was assessed by using an MTT cell viability assay with J774 macrophage ...
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Different mechanisms of two anti anthrax protective antigen antibodies and function comparison between them
... with PA63 separately, they had no competitive relation with PA63. Co-IP, cells and rats’ protective tests dis- played that PA21 (or hmPA6) could not inhibit LF bind- ing to PA63. Consequently, we added PA and ...
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Mechanistic Analysis of the Effect of Deamidation on the Immunogenicity of Anthrax Protective Antigen
... recombinant protective antigen (rPA), we have previ- ously shown that deamidation can decrease the immunogenicity of rPA, the primary component of new-generation anthrax vac- ...
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Role of the Antigen Capture Pathway in the Induction of a Neutralizing Antibody Response to Anthrax Protective Antigen
... Anthrax toxin consists of PA and two catalytically active components, lethal factor (LF) and edema factor (EF). Interaction of PA with LF results in the formation of lethal toxin (LT); interaction of PA with EF ...
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Binding of the von Willebrand Factor A Domain of Capillary Morphogenesis Protein 2 to Anthrax Protective Antigen Vaccine Reduces Immunogenicity in Mice
... Our findings indicate that complexation of sCMG2 with PA increases the thermal stability of PA, as measured by CD, while complexation to sD50ACMG2 has little effect on PA stability. These results are consistent with ...
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Characterization of Dominant-Negative Forms of Anthrax Protective Antigen
... the protective antigen (PA) moiety of anthrax toxin endow the protein with a dominant-negative (DN) phenotype, converting it into a potent ...
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Anthrax Vaccine Antigen-Adjuvant Formulations Completely Protect New Zealand White Rabbits against Challenge with Bacillus anthracis Ames Strain Spores
... improved anthrax vaccine that shows high potency, five different anthrax protective antigen (PA)- adjuvant vaccine formulations that were previously found to be efficacious in a nonhuman ...
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Rapid Point-of-Care Test To Detect Broad Ranges of Protective Antigen-Specific Immunoglobulin G Concentrations in Recipients of the U.S.-Licensed Anthrax Vaccine
... of anthrax-vaccinated individuals in the Armed Forces and others at high ...validated anthrax protective antigen (PA) immu- noglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) and the ...
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Advax-Adjuvanted Recombinant Protective Antigen Provides Protection against Inhalational Anthrax That Is Further Enhanced by Addition of Murabutide Adjuvant
... against anthrax based on recombinant protective antigen (PA) potentially offer more consistent and less reac- togenic anthrax vaccines but require adjuvants to achieve optimal ...pulmonary ...
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Recombinant Vaccine Displaying the Loop-Neutralizing Determinant from Protective Antigen Completely Protects Rabbits from Experimental Inhalation Anthrax
... inhalation anthrax, significant efforts have been directed toward reevaluating our preparedness for possible bioterrorist threats, including weaponized ...the anthrax vaccine currently approved in the ...
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Adenoviral Expression of a Bispecific VHH-Based Neutralizing Agent That Targets Protective Antigen Provides Prophylactic Protection from Anthrax in Mice
... of anthrax, secretes three polypeptides, which form the bipartite lethal and edema toxins (LT and ET, ...toxins, protective antigen (PA), is responsible for binding to cellular receptors and ...
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Neutralizing Activity of Vaccine-Induced Antibodies to Two Bacillus anthracis Toxin Components, Lethal Factor and Edema Factor
... human anthrax vaccine, contains various amounts of the three anthrax toxin components, protective antigen (PA), lethal factor (LF), and edema factor ...against anthrax in animal models, ...
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Antigen Export during Liver Infection of the Malaria Parasite Augments Protective Immunity
... the antigen by cross-presentation via the endosomal pathway, whereas epitope presentation on infected hepatocytes during the effector phase involves antigen secretion to the host cell ...and antigen ...
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A Three-Dose Intramuscular Injection Schedule of Anthrax Vaccine Adsorbed Generates Sustained Humoral and Cellular Immune Responses to Protective Antigen and Provides Long-Term Protection against Inhalation Anthrax in Rhesus Macaques
... class levels were determined for samples that had total anti-PA IgG levels of ⱖ 10 g/ml, had all four subclass IgG ELISAs performed, and had at least one IgG subclass detected. The HuAVA and 1:5 AVA subclass profiles ...
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Anthrax and Gulf War Illness (GWI): Evidence for the Presence of Harmful Anthrax Antigen PA63 In the Serum of Veterans with GWI
... anthrax antibodies or 15% polyclonal anthrax antiserum were added for 2 more days. At the end of the incubation period, the cells were examined for apoptosis. Apoptotic cells were detected using the In Situ ...
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Effective antiprotease antibiotic treatment of experimental anthrax
... Inhalation anthrax is characterized by a systemic spread of the challenge agent, Bacillus ...that anthrax lethal toxin secreted by proliferating bacteria is a major cause of death, however, the pathology of ...
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Recombinant Protective Antigen Anthrax Vaccine Improves Survival when Administered as a Postexposure Prophylaxis Countermeasure with Antibiotic in the New Zealand White Rabbit Model of Inhalation Anthrax
... Inhalation anthrax is a potentially lethal form of disease resulting from exposure to aerosolized Bacillus anthracis ...recombinant protective antigen (rPA) vaccine in combination with an oral ...
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Anti-Protective Antigen IgG Enzyme-Linked Immunosorbent Assay for Diagnosis of Cutaneous Anthrax in India
... India, anthrax is still enzootic; however, more sporadic cases of human anthrax are reported from southern states than from northern states ...Pradesh, anthrax is endemic and a public health problem ...
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