Anti-CD28
The Mode and Duration of Anti-CD28 Costimulation Determine Resistance to Infection by Macrophage-Tropic Strains of Human Immunodeficiency Virus Type 1 In Vitro
... by anti-CD3/anti-CD28 antibodies immobilized on beads and that on the surface of a tissue culture plate, or if the effects are simply due to the time of exposure to the ...to ...
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Comparison of anti CD3 and anti CD28 coated beads with soluble anti CD3 for expanding human T cells: Differing impact on CD8 T cell phenotype and responsiveness to restimulation
... biotinylated anti-CD3 and anti-CD28 anti- ...FITC-labeled anti-mouse IgG and flow cyto- metry to monitor biotinylated antibody binding to beads, established that beads were saturated by ...
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Endogenous analgesia mediated by CD4+ T lymphocytes is dependent on enkephalins in mice
... Anti- β -endorphin polyclonal IgG antibodies cross-react with enkephalins expressed in activated T lymphocytes Purified naive wild-type CD4 + T lymphocytes were stim- ulated with a cocktail of anti-CD3 and ...
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A profile of immune response to herpesvirus is associated with radiographic joint damage in rheumatoid arthritis
... bilized anti-CD3 and anti-CD28 monoclonal antibodies (anti-CD3/anti-CD28; Dynabeads Human T-Activator, Invitrogen, Grand Island, NY, USA); bacterial CpG oli- gonucleotides (CpG); ...
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Adjuvant effect of anti-4-1BB mAb administration in adoptive T cell therapy of cancer
... of anti-4-1BB mAb has been found to be a potent adjuvant when combined with other therapeutic approaches, ...therapies, anti-OX40 therapy, and peptide or DC ...of anti-4-1BB mAb administration in ...
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PAG/Cbp suppression reveals a contribution of CTLA-4 to setting the activation threshold in T cells
... an anti-CD3+anti-CD28 coated plastic ...on anti-CD3+anti-CD28 coated plastic plate, lysed and CTLA-4 was ...on anti-CD3+anti-CD28 coated plastic plate, lysed ...
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Disulfiram Reactivates Latent HIV-1 in a Bcl-2-Transduced Primary CD4+ T Cell Model without Inducing Global T Cell Activation
... To confirm the effect of disulfiram on latent HIV-1, we examined levels of HIV-1 transcripts before and after disul- firam stimulation. Multiply spliced transcripts encoding Tat and full-length HIV-1 transcripts were ...
5
Histamine receptor H1 is required for TCR mediated p38 MAPK activation and optimal IFN γ production in mice
... and anti–IL-4 mAb (10 μ g/ml), (B) in the presence of IL-4 (30 ng/ml) and anti–IFN-γ mAb (10 μg/ml), or (C) in the presence of TGF-β (1 ng/ml), IL-6 (30 ng/ml), and anti–IFN-γ (10 μg/ml) and ...
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The role of human umbilical cord tissue derived mesenchymal stromal cells (UCX®) in the treatment of inflammatory arthritis
... with anti-CD3, anti-CD28 and IL-2 while co-cultured with irradiated UCX ® cells, bone marrow-derived mesen- chymal stem cells (BM-MSCs) and tumor cells belonging to an acute lymphoblastic leukemia ...
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TSC1 regulates the balance between effector and regulatory T cells
... tions for Th1 or Th17 cell differentiation, including coincuba- tion with appropriate cytokines and anticytokine antibodies for 5 days, followed by restimulation with anti-CD3 and anti-CD28. ...
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A non-canonical adenosinergic pathway led by CD38 in human melanoma cells induces suppression of T cell proliferation
... with anti-CD3/anti-CD28 beads (Myltenyi Biotec) following manufacturer’s ...with anti-Phospho-Raptor or anti-Phospho-Rictor rabbit polyclonal antibodies (Cell Signaling, MA, ...goat ...
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The Effects of Anti-CD3/CD28 Coated Beads and IL-2 on Expanded T Cell for Immunotherapy
... with anti-CD3 and anti-CD28, which mimic professional APCs, can be a convenient reagent for the expansion of T lymphocytes in vitro [5, ...with anti-CD3/CD28- -coated beads, several ...
8
Selective CD28 blockade attenuates CTLA-4–dependent CD8+ memory T cell effector function and prolongs graft survival
... Ig, anti-CD28 dAb alone, or anti-CD28 dAb in the presence of blocking antibodies against ...selective CD28 blockade better inhibited the acquisition of IFN-γ, TNF, and IL-2 production ...
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Selective Accumulation of Th2-skewing Immature Erythroid Cells in Developing Neonatal Mouse Spleen
... and anti-CD28 monoclonal antibodies (Abs) in the absence or presence of purified immature erythroid cells (nucleated Ter119 + cells) from spleen of ...
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Determination of antigen specific memory/effector CD4+ T cell frequencies by flow cytometry: evidence for a novel, antigen specific homeostatic mechanism in HIV associated immunodeficiency
... 1 anti-CD28 and then examined for their correlated expression of cytokine (g-IFN, IL-2, TNF-a, and IL-4 for CMV; g-IFN for mumps virus) versus CD69 ver- sus CD4 with net effector frequen- cies determined as ...
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CD28 ligation induces transplantation tolerance by IFN γ–dependent depletion of T cells that recognize alloantigens
... same CD28-specific Ab inhibited T cell expansion and cytokine production after stimulation with superantigen (18) or peptide antigen ...that anti–CD28 mAb prevents GVHD in mice (20), and Dengler et ...
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Macrophages suppress T cell responses and arthritis development in mice by producing reactive oxygen species
... with anti-CD3 or mitogens did not show differences in activation thresh- olds, suggesting that this imprinting in vivo is antigen ...with anti-CD3 and anti-CD28 in presence of ROS, a clear Th2 ...
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Regeneration and Tolerance Factor Prevents Bystander T-Cell Death Associated with Human Immunodeficiency Virus Infection
... apoptosis. Anti-RTF antibodies mediate apoptosis in T lymphocytes. When anti-RTF was added to lymphocytes from an HIV- positive individual, they underwent larger amounts of apoptosis than cells taken from ...
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Downregulation of CD40 Ligand Response in Monocytes from Sepsis Patients
... (53). CD28/CD80-CD86 interactions play a central role in providing costimulatory signals to T cells ...of CD28 by CD80 and/or CD86 has been shown to induce T-cell production of growth factors and T-cell ...
8
Transcriptome Analysis of CD4+ T Cells in Coeliac Disease Reveals Imprint of BACH2 and IFN? Regulation
... PCA analysis (Fig 1) of the normalised expression data showed that the unstimulated sam- ples do not form any distinct groups indicating that overall gene expression patterns do not dif- fer between case and control ...
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