antibody assays
Field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of China
... immunodiagnostic antibody assays (IHA, ELISA and DDIA), and the immunodiagnostic antigen assays (ELISA (IgY) and ELISA (COM)) need collection of whole blood samples via ...of antibody ...
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Comparison of Six Automated Treponema Specific Antibody Assays
... Results of automated immunoassays. A total of 157 samples from 155 patients (median age, 56 years; range, 19 to 93 years) and 2 standards showed positive results. The overall percentages of agreement and corresponding ...
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Microsphere based antibody assays for human parvovirus B19V, CMV and T gondii
... The B19V SIA was performed following the manufac- turer’s protocol (“Sample protocol for indirect antibody capture immunoassay”). The B19V SIA was optimized with serial serum dilutions ranging from 1:5 to 1:320 in ...
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Correlation between TSH Receptor Antibody Assays and Clinical Manifestations of Graves' Orbitopathy
... Patients in this study were examined at the department of ophthalmology, Severance Hospital of Yonsei University College of Medicine between January 2008 and December 2010. All subjects were diagnosed with GO and a ...
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Highly Accurate Antibody Assays for Early and Rapid Detection of Tuberculosis in African and Asian Elephants
... rapid antibody detection in ...robust antibody responses to multiple antigens of ...serologic assays demonstrated 100% sensitivity and 95 to 100% ...accurate antibody tests to identify ...
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Evaluation of Serum Bactericidal Antibody Assays for Haemophilus influenzae Serotype a
... SBA assays were determined by Pearson’s product moment correlation coefficient by use of Sigma Plot and Sigma Stat software, version ...among assays were determined by Student’s t test or the Mann-Whitney ...
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Comparison of fluorescent antibody, neutralizing antibody, and complement enhanced neutralizing antibody assays for detection of serum antibody to respiratory syncytial virus
... A comparison of three assays for the detection of serum antibody to respiratory syncytial virus RSV was carried out on 47 serum samples obtained sequentially from infants and young child[r] ...
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Comparison of Real Time PCR Assays with Fluorescent Antibody Assays for Diagnosis of Respiratory Virus Infections in Children
... Clinical specimens. From October 2003 through September 2004, 1,138 con- secutive specimens (1,074 nasal wash samples, 14 nasal swabs, 44 tracheal aspi- rates, and 6 bronchoalveolar lavage [BAL] specimens) submitted to ...
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Use of aminotransferase, hepatitis C antibody, and hepatitis C polymerase chain reaction RNA assays to establish the diagnosis of hepatitis C virus infection in a diagnostic virology laboratory
... To evaluate the ability of a diagnostic laboratory to accurately identify such patients, we compared results obtained with serum transaminase assays, two HCV antibody assays enzyme immun[r] ...
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Comparison of seven enzyme immunoassay systems for measurement of cytomegalovirus
... In the case of the single-antibody assays, the sandwich method, in which the goat anti-CMV was directly labeled with enzyme, was the most sensitive, allowing for the detection of 1:1,600[r] ...
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Fourth Generation Enzyme Linked Immunosorbent Assay for the Simultaneous Detection of Human Immunodeficiency Virus Antigen and Antibody
... HIV antibody as well ...of assays may be insensitive levels of detection, because low concentrations of antigen are difficult to detect and antigenemia occurs only transiently dur- ing different stages of ...
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Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species
... Conservation of potential serodiagnostic proteins across Leishmania species. We previously reported high sensitivity and specificity for the serodiagnosis of leish- maniasis using L. (L.) infantum rHsp83 (12). While this ...
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2,8-Diazaspiro[4.5]decan-8-yl)pyrimidin-4-amine potent CCR4 antagonists capable of inducing receptor endocytosis
... the biological assays was examined by LCMS analysis and was found to be 95%, unless otherwise specified. All animal studies were ethically reviewed and carried out in accordance with Animals (Scientific ...
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Antinuclear antibody prevalence in a general pediatric cohort from Mexico City: discordance between by immunofluorescence and multiplex assays
... Moreover, our data demonstrate that the performance characteristics of the 2 ANA assays varied between boys and girls, with lower agreement among girls, where the coefficient of agreement was classified as ...
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Development and Technical and Clinical Validation of a Quantitative Enzyme-Linked Immunosorbent Assay for the Detection of Human Antibodies to Hepatitis B Surface Antigen in Recipients of Recombinant Hepatitis B Virus Vaccine
... Finally, regression was considered as good between the anti- HBs in-house assay and the AUSAB RIA. Fuller’s coefficient of correlation (simple correction on the AUSAB RIA) was calculated to be 0.9815, which can be ...
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Mycoplasma agassizii Strain Variation and Distinct Host Antibody Responses Explain Differences between Enzyme-Linked Immunosorbent Assays and Western Blot Assays
... other assays, such as Western blot, met- abolic inhibition, and complement fixation, are documented to be strain dependent or best used for confirmation (1, 12, 16, 17, 22, 40, ...tional assays, and in vivo ...
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Detection of Active Infection in Nonhuman Primates with Lyme Neuroborreliosis: Comparison of PCR, Culture, and a Bioassay
... the antibody response and allow a small in- oculum to establish ...immunosorbent assays (ELISAs) or immunoblots were positive, the mice were sacrificed and the hearts were ...
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Antibody responses to the capsular polysaccharide of Neisseria meningitidis serogroup B in patients with meningococcal disease.
... PS antibody concentrations is illustrated by the results of assays of 13 representative acute- or convalescent-phase serum samples from patients with MenB disease (Table ...sample, antibody ...
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Interactions between Natural Killer Cells and Antibody Fc Result in Enhanced Antibody Neutralization of Human Immunodeficiency Virus Type 1
... by antibody was enhanced when target cells for virus growth were monocyte-depleted, peripheral blood mononu- clear cells (PBMCs) rather than CD4 ⴙ ...enhanced antibody inhibition, whereas adding NK cells to ...
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S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products
... monoclonal antibody (5 μg/ml) or control IgG (5 μg/ml, Isotype Control-Goat IgG (Fc), Abcam, Cambridge, UK) for 30 min before the addition of rS100A8 (1 μg/ml) and ...
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