Antibody repertoire
High-throughput Sequencing and Profiling of Antibody Repertoire and the Promises for Diagnostic and Therapeutic Applications
... Recombinant antibody technology is opening new point of view for the advances of novel therapeutic and diagnostic ...possible antibody preparation using gene engineering ...of antibody repertoires is ...
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Preferential selection of receptor-binding variants of influenza virus hemagglutinin by the neutralizing antibody repertoire of transgenic mice expressing a human immunoglobulin mu minigene.
... neutralizing antibody repertoire, for influenza virus hemagglutinin (HA) of transgenic mice expressing a human immunoglobulin m (IgH) minigene, by monoclonal antibody (MAb) selection and sequencing ...
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Mining the pre diagnostic antibody repertoire of TgMMTV neu mice to identify autoantibodies useful for the early detection of human breast cancer
... Genetically engineered mouse models of cancer may provide a model system for identification of diagnostic autoantibodies. As mice develop spontaneous breast can- cers, many in middle age, serum can be collected over time ...
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Heterosubtypic Immunity to Influenza A Virus Infection Requires a Properly Diversified Antibody Repertoire
... receptor repertoire and ⌬ D-iD mice with an altered antibody repertoire failed to develop complete HSI against challenge with a heterologous influenza virus ...
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The systemic lupus erythematosus VλJλ repertoire harbors characteristics of the natural antibody repertoire
... Department of Medicine III, Institute for Clinical Immunology, University of Erlangen–Nuremberg, Erlangen, Germany Arthritis Res Ther 2003, 5(Suppl 3):95 (DOI 10.1186/ar896) A complex cellular system like the human ...
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Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes
... native antibody HC-LC pairs with high pairing accuracy Our approach is based on the concept that each B cell contains rough endoplasmic reticulum (rER) with bound ribosomes for co-transcriptional translocation of ...
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Characterization and Epitope Mapping of the Polyclonal Antibody Repertoire Elicited by Ricin Holotoxin-Based Vaccination
... of antibody- bound phages was enriched by a factor of 30, indicating successful ...the antibody-binding phages, in all three libraries, con- tained the motif LPNR ...
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Statistical inference of a convergent antibody repertoire response to influenza vaccine
... Spe- cifically, for a given patient we randomly chose a time- point, then within this time-point, we selected one CDR3 sequence from the B cell dataset and one from the PBMC dataset, whe[r] ...
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Live virus exposure of vaccine protected macaques alters the anti HIV 1 antibody repertoire in the absence of viremia
... the antibody (Ab) responses in vaccinees that had resisted all mucosal challenges completely and asked two ques- tions: i) is there a quantitative difference in the anti-Env Ab titers after versus before ...
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Restricted immunoglobulin VH usage and VDJ combinations in the human response to Haemophilus influenzae type b capsular polysaccharide Nucleotide sequences of monospecific anti Haemophilus antibodies and polyspecific antibodies cross reacting with self antigens
... human antibody repertoire generated against a biologically significant antigen we have obtained sequences of heavy chain variable region genes (IgVH) from 15 monoclonal antibodies specific for the capsular ...
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Persistence and selection of an expanded B cell clone in the setting of rituximab therapy for Sjögren’s syndrome
... normal antibody repertoire, reportedly occurring in approximately 1/800 rearrangements from the blood B cells of healthy individuals [53]); however, D-D fusions may be more common in autoimmune strains of ...
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Regeneration of the immunoglobulin heavy chain repertoire after transient B cell depletion with an anti CD20 antibody
... B-cell repertoire is shaped by a complex set of gene rear- rangements, somatic hypermutation and receptor-driven ...the repertoire is increased by the addition of or the deletion of nucleotides at the ...
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DNA demethylating agent decitabine broadens the peripheral T cell receptor repertoire
... The frequencies of the top 10 V-J combinations in these 4 patients before and after decitabine therapy are shown in Figure 3. The detailed information of amino acid and DNA sequences of CDR3 for the highest expansion ...
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The gestural repertoire of the wild chimpanzee
... species-typical repertoire, most simply explained as a result of genetical channelling, it remains the case that these gestures are used in a highly inten- tional ...this repertoire by learning new ges- ...
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Quantitative characterization of T-cell repertoire and biomarkers in kidney transplant rejection
... Results: Seven patients developed TCMR during the first three months of the study. Out of six patients who had complete sets of blood and biopsy samples two had TCMR. We found an expansion of the TCR repertoire in ...
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Broadly Reactive Anti-Respiratory Syncytial Virus G Antibodies from Exposed Individuals Effectively Inhibit Infection of Primary Airway Epithelial Cells
... by antibody epitope ...that antibody-induced inhibition of the RSV G protein interaction with the CX3CR1 receptor does prevent viral entry into airway epithelial ...protein-specific antibody (3, 33), ...
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Isolation and Characterization of Human Monoclonal Antibodies from Individuals Infected with West Nile Virus
... Reformatting of scFv phage into full-length IgG1 molecules has been de- scribed (3). Briefly, variable heavy (VH) and light (VL) genes were PCR ampli- fied using primers to restore the human framework and append ...
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The human olfactory receptor repertoire
... shown in the multiple sequence alignment (Figure 2) are those extracted from genomic sequence database entries. Studies of human OR gene and pseudogene sequences cover- ing approximately 150 full-length receptor genes ...
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Stochastic competitive exclusion in the maintenance of the naïve T cell repertoire
... Recognition of antigens by the adaptive immune system relies on a highly diverse T cell receptor reper- toire. The mechanism that maintains this diversity is based on competition for survival stimuli; these stimuli ...
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