Antifreeze protein
Polyproline is a minimal antifreeze protein mimetic and enhances the cryopreservation of cell monolayers
... Herein, we introduce polyproline as a minimum (bio)- synthetic antifreeze protein mimic. We demonstrate that polyproline has ice recrystallisation inhibition activity, which is linked to its amphipathic PP ...
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Cloning, expression, and activity of type IV antifreeze protein from cultured subtropical olive flounder (Paralichthys olivaceus)
... require antifreeze activity for ...discernible antifreeze activity although ana- lysis of its expressed sequence tags (ESTs) revealed an EST encoding a putative type IV antifreeze protein (AFP ...
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Effect of Antifreeze Protein on Mouse Ovarian Tissue Cryopreservation and Transplantation
... Purpose: To investigate the effect of antifreeze protein (AFP) supplementation on ovarian vitrification and transplantation. Materials and Methods: In this experi- mental study, we researched a total of 182 ...
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Extracellular antifreeze protein significantly enhances the cryopreservation of cell monolayers
... III antifreeze protein from ocean pout (AFPIII) was ...an antifreeze protein, as opposed to polymeric cryoprotectants, was their high IRI activity (capable of inhibiting all ice growth well ...
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Improvement in Ovarian Tissue Quality with Supplementation of Antifreeze Protein during Warming of Vitrified Mouse Ovarian Tissue
... Ice easily recrystallizes during warming after vitrification, and antifreeze protein (AFP) can inhibit the re-crystallization. However, no study has evaluated the effect of AFP treatment only thereon during ...
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BIOPHYSICAL PROPERTIES OF AN ANTIFREEZE PROTEIN AND THE EFFECTS OF IONIC LIQUIDS ON THE MODEL PROTEIN GB1, Korth Wade Elliott
... of protein characterized by their ability to depress the freezing point of water sufficient to prevent the formation of ice crystals by adsorbing to the surface of ice ...science. Antifreeze proteins have ...
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Antifreeze protein mimetic metallohelices with potent ice recrystallization inhibition activity
... ABSTRACT: Antifreeze proteins are produced by extremophile species to control ice formation and growth, and they have potential applications in many fi ...metallo-organic antifreeze protein mimetics ...
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The rational design and applications of synthetic antifreeze protein mimics
... of antifreeze protein mimetic macromolecules and may help shed light on their underlying mechanisms of action as well as developing ‘minimum synthetic mimics’ of ...
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Rational, yet simple, design and synthesis of an antifreeze protein inspired polymer for cellular cryopreservation
... We have demonstrated a new, facile, biomimetic approach to engineering ice-recrystallization inhibiting polymers, inspired by antifreeze proteins. A region-regular poly(ampholyte) was synthe- sized from a bulk ...
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Enhancement of insect antifreeze protein activity by solutes of low molecular mass
... Antifreeze proteins (AFPs) lower the non-equilibrium freezing point of water (in the presence of ice) below the melting point, thereby producing a difference between the freezing and melting points that has been ...
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Suppression of droplets freezing on glass surfaces on which antifreeze polypeptides are adhered by a silane coupling agent
... The present authors have focused on three polypeptides, synthesized by Kun and Mastai [19] based on segments of winter flounder antifreeze protein, as the alternatives. Kun and Mas- tai analyzed the ...
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Type-IV Antifreeze Proteins are Essential for Epiboly and Convergence in Gastrulation of Zebrafish Embryos
... of antifreeze proteins (AFPs). Type-IV antifreeze proteins (AFP4s) are present also in certain temperate and even tropical fish, which has raised a question as to whether these AFP4s have important ...
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Site specific conjugation of antifreeze proteins onto polymer stabilized nanoparticles
... and protein engineering to enable precision-conju- ...distal protein capture (nickel NTA or O 6 -benzyl-guanine) moiety to be ...free antifreeze proteins on a valency-corrected basis confirming ...
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Gold nanoparticle aggregation as a probe of antifreeze (glyco) protein inspired ice recrystallization inhibition and identification of new IRI active macromolecules
... in antifreeze protein assays (although at lower concentrations than used here) this should be taken into account in the future as it clearly does have activity, although the features that cause this are not ...
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LIFE HISTORY AND ENVIRONMENTAL TOLERANCE OF THE INVASIVE ORIENTAL WEATHERFISH ( Misgurnus anguillicaudatus IN SOUTHWESTERN IDAHO, USA
... Type II antifreeze protein from a mid-latitude freshwater fish, Japanese smelt (Hypomensus nipponensis).. Model selection of parameters affecting fish mortality of oriental weatherfish[r] ...
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PLANT GROWTH MODELING OF ZINNIA ELEGANS JACQ USING FUZZY MAMDANI AND L SYSTEM APPROACH WITH MATHEMATICA
... of antifreeze solutions, including water, sodium chloride, calcium chloride, methanol, ethanol, ethylene glycol, potassium acetate, potassium carbonate, ...Each antifreeze solution has its own ...
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Effects of antifreeze proteins on red blood cell survival during cryopreservation
... I protein, for the current studies we used the same experimental conditions that we had employed previously (Carpenter and Hansen, ...(M1.1) protein are shown in ...I protein (Carpenter and Hansen, ...
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Antifreeze activities of various fungi and Stramenopila isolated from Antarctica
... Isolates were cultured in liquid media (potato dextrose broth, PDB; Difco and PYG broth) at - 1˚C for 1-2 months. The antifreeze activities of each culture medium were further examined by observation of ice ...
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The Role of the Thermal Hysteresis Factor in Tenebrio Molitor Larvae
... The antifreeze function of the thermal-hysteresis-producing factor does not account for the 'low' levels of the factor in larvae reared under summer temperature and photoperiod condition[r] ...
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Short circuit co evolution by the perfect parasites : antifreeze glycoproteins in Antarctic fish leeches (Hirudinea, Piscicolidae) : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Zoology at Massey University
... Short Circuit Co-Evolution by the Perfect Parasites: Antifreeze Glycoproteins in Antarctic Fish Leeches Hirudinea, Piscicolidae}.. A thesis presented in partial fulfilment of the require[r] ...
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