Antigen-capture ELISA
Establishment of antigen capture ELISA detection method for H9 subtype avian influenza virus HA protein
... establish antigen capture ELISA detection method for H9 subtype avian influenza ...by ELISA coated with the AIV antigen, an antigen capture ELISA based on ...
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Use of monoclonal antibodies against Hendra and Nipah viruses in an antigen capture ELISA
... The design and setup of antigen capture ELISA for heni- pavirus were based on the assay developed for Ebola virus as described previously [43,50]. Five μg/mL of monoclo- nal antibody (1A11 C1 or 2B10 ...
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EVALUATION OF NS1 ANTIGEN CAPTURE ELISA AND IgM ANTIBODY CAPTURE ELISA IN ACUTE CASES OF DENGUE IN RURAL POPULATION IN A TERTIARY CARE TEACHING HOSPITAL IN WESTERN UTTAR PRADESH INDIA
... antibody capture are available with different sensitivities and specificities observed in various ...(NS1) antigen-capture ELISA against IgM antibody capture ELISA to confirm ...
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Development and application of an antigen capture ELISA assay for diagnosis of Japanese encephalitis virus in swine, human and mosquito
... the ELISA assay developed in this study has following potential ...our ELISA assay does not require expensive reagents and special facilities, and it can be routinely performed in any ordinary laboratory ...
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Detection of Asymptomatic Antigenemia in Pigs Infected by Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by a Novel Capture Immunoassay with Monoclonal Antibodies against the Nucleocapsid Protein of PRRSV
... N antigen capture ...of capture and detector antibodies for an antigen capture ELISA, MAbs were paired according to their ability to recognize different epitopes on the N ...each ...
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Characterization of a Lipoprotein Common to Legionella Species as a Urinary Broad Spectrum Antigen for Diagnosis of Legionnaires' Disease
... PAL antigen in infected urine specimens, which is useful for the diagnosis of Legionnaires’ ...PAL antigen capture enzyme-linked immunosorbent assay (ELISA) to detect urinary PAL ...
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Monoclonal Antibody-Based Antigen Capture Enzyme-Linked Immunosorbent Assay Reveals High Sensitivity of the Nucleocapsid Protein in Acute-Phase Sera of Severe Acute Respiratory Syndrome Patients
... SARS-CoV antigen in sera may provide a possible method for early diagnosis of ...an antigen-capture ELISA based on monoclonal antibodies (MAbs) against the N protein of SARS-CoV ...
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Full Serotype- and Group-Specific NS1 Capture Enzyme-Linked Immunosorbent Assay for Rapid Differential Diagnosis of Dengue Virus Infection
... NS1 antigen capture ...of capture and detecting MAbs for serotype-specific DENV3- and DENV4-specific NS1 capture ELISAs and a group-specific NS1 antigen capture ELISA, ...
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Serotype 1 Specific Monoclonal Antibody Based Antigen Capture Immunoassay for Detection of Circulating Nonstructural Protein NS1: Implications for Early Diagnosis and Serotyping of Dengue Virus Infections
... specific antigen capture enzyme-linked immunosorbent assay (ELISA) for early detection and serotyping of DV serotype 1 (DV1) by using well-characterized monoclonal antibodies (MAbs) specific to ...
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Development of an Antigen Capture Immunoassay Based on Monoclonal Antibodies Specific for Dengue Virus Serotype 2 Nonstructural Protein 1 for Early and Rapid Identification of Dengue Virus Serotype 2 Infections
... target antigen for diagnosis (1, 27, 35). Recently, two DENV NS1 antigen capture immunoassays have become commercially available for the early diagnosis of dengue virus (2, 3, 7, ...NS1 ...
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Detection of human leptospirosis as a cause of acute fever by capture ELISA using a Leptospira interrogansserovar Copenhageni (M20) derived antigen
... In 1990, the United States Naval Medical Research Unit No. 6 (NAMRU-6) established a passive surveillance network for the study of acute febrile syndromes in the Peruvian Amazon, with expansion into Bolivia, Paraguay, ...
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Single Antigen Detects both Immunoglobulin M (IgM) and IgG Antibodies Elicited by All Four Dengue Virus Serotypes
... whole-virus antigen prepared from infected suckling mouse brain or insect cells in culture and needs paired acute- and convalescent-phase ...antibody capture–enzyme-linked im- munosorbent assay ...
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Evaluation of serological methods for diagnosis of Puumala hantavirus infection (nephropathia epidemica)
... coli-expressed antigen (aa 1 to 119) in a direct- coating ...Progen ELISA is based on PUU virus strain CG-1820, which, in con- trast to Swedish or central European PUU virus strains, differs from strain ...
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Adaptation of Very Virulent Infectious Bursal Disease Virus to Chicken Embryonic Fibroblasts by Site-Directed Mutagenesis of Residues 279 and 284 of Viral Coat Protein VP2
... 11 were used in antigen capture enzyme-linked immunosorbent assays ELISA to examine their immunoreactivities to several CEF-nonpermissive IBDV strains isolated from Hong Kong and China 3[r] ...
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Evaluation of a commercial Erns-capture ELISA for detection of BVDV in routine diagnostic cattle serum samples
... the ELISA, and all 90 samples cat- egorized as virus positive were tested positive, ...the ELISA was 100% in relation to IPX, and the agreement between the tests was perfect (k = ...
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Monoclonal antibody passive hemagglutination and capture enzyme linked immunosorbent assays for direct detection and quantitation of F41 and K99 fimbrial antigens in enterotoxigenic Escherichia coli
... The capture ELISA systems we developed use rabbit polyclonal antibodies for antigen capture and monoclonal antibody preparations for antigen detection in ETEC cultures and in bovine feca[r] ...
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Monoclonal antibody based capture ELISA in the diagnosis of previous dengue infection
... antibody ELISA [12] and hemagglutination inhibition ...antibody-based capture enzyme-linked immunosorbent assay (MAb-ELISA) in the assessment of dengue ...
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Mapping of B-cell determinants in the nucleocapsid protein of Puumala virus: definition of epitopes specific for acute immunoglobulin G recognition in humans.
... an ELISA based on native PUU virus antigen, and the results were compared with the peptide data (Table ...native antigen but IgG positive in the N11-BSA assay were both drawn 4 days after onset of ...
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Diagnostic Potential and Antigenic Properties of Recombinant Tick Borne Encephalitis Virus Subviral Particles Expressed in Mammalian Cells from Semliki Forest Virus Replicons
... After an incubation period of 7 to 14 days, the transmission of TBEV can cause febrile illness lasting for 4 to 10 days in the in- fected individual, followed by a symptomless interval of a few days, as well as ...
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Antigen Capture Competitive Enzyme Linked Immunosorbent Assays Using Baculovirus Expressed Antigens for Diagnosis of Bluetongue Virus and Epizootic Hemorrhagic Disease Virus
... viral antigen for these tests is laborious and variable from batch to batch, and the resulting antigen may be ...in antigen capture c-ELISAs (Ag Cap c-ELISA) tests that specifically ...
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