Apoptosis and cell cycle analysis
Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis
... while cell colony formation assay showed that gramicidin significantly inhibited the proliferation of gastric cancer cells, but had little effect on normal gastric mucosal ...SGC-7901 cell. Meanwhile, ...
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The Regulation Role of PBK in Cell Cycle and Apoptosis of Prostate Cancer
... Cell Cycle Assay: The DU145 and LNCaP cells were infected with lentivirus for 48 hours before ...For cell cycle analysis, 1×106 cells were harvested ， centrifuged without supernatant ...
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Cell Cycle Analysis By Flow Cytometry Protocol
... detailed analysis of core cell cycle and apoptosis status in relieve and mouse pluripotent cells, yet amazingly informative ...facs analysis workflow can fix a simple, cj and is called ...
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Cell cycle arrest and apoptosis induction by methanolic leaves extracts of four Annonaceae plants
... cancer cell lines, includ- ing human cervical carcinoma (HeLa, SiHa, and CaSki), human hepatocellular carcinoma (HepG2 and Hep3B), and human myeloid leukemia (K562, U937, and ...apoptotic cell death in ...
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The cell type-specific effect of TAp73 isoforms on the cell cycle and apoptosis
... The analysis of the gene expression associated with a p53-mediated signal transduction ...detailed analysis of every single gene is outside the remit of this report, and only the group of genes involved in ...
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Prognostic factors correlation between the cell cycle phases and apoptosis in basal cell carcinoma
... cine were studied. Samples from patients with suspected BCC were collected thought 4 - 5 mm punch biopsy. Sur- gical excision of the tumor was performed in confirmed cases of BCC and/or small lesions. Tumor fragments ...
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Original Article Analysis of apoptosis and cell cycle of lymphocyte subsets in peripheral blood of patients with ankylosing spondylitis
... Tn cell proliferation was increased and apoptosis was decreased, which mainly differ- entiated into CD4+ T cells with a predominance of Th1 cells, whereas, few Tn cells differentiated into Treg cells, ...
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Identification of deregulation of apoptosis and cell cycle in neuroendocrine tumors of the lung via NanoString nCounter expression analysis
... Between SCLC and LCNEC CDK2, CDKN1B, CDKN2A and PNN expression was significantly different with higher expression in SCLC. Conclusion: Carcinoids have increased CDK4/6 and CCND1 expression controlling RB1 phosphorylation ...
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A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells
... on cell cycle can be done by performing a proliferation growth curve to show dif- ferences more ...Blot analysis should reveal a protein expression for the main cell cycle proteins as ...
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Cell Cycle Exit: Growth Arrest, Apoptosis, and Tumor Suppression Revisited
... group of basic helix-loop-helix (HLH) factors, which induce muscle differentiation and cause growth arrest long before induction of muscle proteins.. Careful analysis of the region invol[r] ...
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Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
... examined. Analysis of Cellular DNA Content Using Propidium Iodide HeLa cells at a concentration of 1x10 6 cells/ml were seeded into 6-well plate in 2 ml culture medium with a concentration of IC 50 value of ...
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Nonstructural Protein σ1s Mediates Reovirus-Induced Cell Cycle Arrest and Apoptosis
... in cell cycle arrest at the G 2 /M boundary and induction of ...in cell cycle arrest and apoptosis, we used reverse genetics to generate a 1s-null ...of apoptosis than the ...
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Ursolic acid induces cell cycle arrest and apoptosis of gallbladder carcinoma cells
... several apoptosis-related proteins, including PARP, caspase-3, caspase-9, cyto- chrome c, Bax and Bcl-2, by western blot ...both cell lines, suggesting that these proteins are in- volved in UA-induced ...
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Capsaicin induces cell cycle arrest and apoptosis in human KB cancer cells
... cancer cell lines ...blot analysis indicated the involvement of caspase 9 in capsaicin-induced apoptosis of KB cells (Figure ...of apoptosis, the extrinsic pathway, is initiated by the binding ...
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Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells
... blot analysis After experimental treatment, total protein was isolated on ice and subjected to 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis gels using modified radio immunoprecipitation assay ...
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Telomere Loss in Somatic Cells of Drosophila Causes Cell Cycle Arrest and Apoptosis
... a cell cycle checkpoint determinant following Toczyski, ...point. Cell 90: 1097–1106. and molecular analysis of terminal deletions of chromosome 3R Vaziri, ...
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Online Flow Cytometry Protocol for Monitoring Apoptosis, Cell Cycle and Viable Cell Number in Suspension Mammalian Cell Cultures
... calibrated cell size and viable cell number ...viability analysis and that the G 1 peak is fixed at a specific channel number so that a minimum of 10,000 nuclear signals are ...
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Casticin induces apoptosis and G0/G1 cell cycle arrest in gallbladder cancer cells
... in apoptosis initiation and maintenance [16, ...casticin-induced apoptosis using western blot ...evaluated cell viability after treatment with casticin in the presence or absence of Z-VAD-FMK, a ...
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MLN4924 suppresses neddylation and induces cell cycle arrest, senescence, and apoptosis in human osteosarcoma
... comparative analysis of NAE1 and Ube2M levels in OS tumor tissues and in healthly bone ...OS cell survival and suggest that MLN4924- induced inhibition of neddylation might help manage OS in most ...
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Depletion of thymopoietin inhibits proliferation and induces cell cycle arrest/apoptosis in glioblastoma cells
... dataset analysis was used to explore the expression level of TMPO in GBM ...blot analysis. Furthermore, the effects of TMPO silencing on GBM cell proliferation and apoptosis were examined by ...
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