ARCHAEON SULFOLOBUS
The Role of cis-acting Sequences Governing Catabolite Repression Control of lacS Expression in the Archaeon Sulfolobus solfataricus
... The archaeon Sulfolobus solfataricus uses a catabolite repression-like system to control production of several glycoside hydrolases. To better understand this regulatory system, studies of the regulation of ...
10
CRISPR mediated targeted mRNA degradation in the archaeon Sulfolobus solfataricus
... The recently discovered clustered regularly interspaced short palindromic repeat (CRISPR)- mediated virus defense represents an adaptive immune system in many bacteria and archaea. Small CRISPR RNAs cause cleavage of ...
9
Extragenic Pleiotropic Mutations That Repress Glycosyl Hydrolase Expression in the Hyperthermophilic Archaeon Sulfolobus solfataricus
... hyperthermophilic archaeon Sulfolobus solfataricus employs a catabolite repression-like regulatory system to control enzymes involved in carbon and energy ...
10
Virus Induced Dormancy in the Archaeon Sulfolobus islandicus
... concentrated S. islandicus strain Y08.82.36 (see Table S1), a highly suscep- tible Sulfolobus islandicus host isolated from Yellowstone National Park. Cells mixed with virus dilutions were plated on overlays of ...
8
The Mre11 protein interacts with both Rad50 and the HerA bipolar helicase and is recruited to DNA following gamma irradiation in the archaeon Sulfolobus acidocaldarius
... In order to check potential interactions between Rad50, Mre11, HerA, NurA and RadA proteins, we performed immuno-precipitation assays using total extracts of expo- nentially growing Sulfolobus cells. As shown in ...
10
Lesion-Induced Mutation in the Hyperthermophilic Archaeon Sulfolobus acidocaldarius and Its Avoidance by the Y-Family DNA Polymerase Dbh
... hyperthermophilic archaeon was reported to catalyze TLS in vitro (Jozwiakowski et ...to Sulfolobus genomes from 8-oxo-dG, which, to our knowl- edge, has not been predicted by other ...
22
Redox stress proteins are involved in adaptation response of the hyperthermoacidophilic archaeon Sulfolobus solfataricus to nickel challenge
... crenarchaeon Sulfolobus solfataricus, which grows between 70 and 90°C and in a pH range of 2–4 [10], is an attractive crenarchaeal model organism for functional genomic ...the Sulfolobus craenar- chaeon ...
11
Transcriptome Analysis of Infection of the Archaeon Sulfolobus solfataricus with Sulfolobus Turreted Icosahedral Virus
... of Sulfolobus acidocaldarius, a close rel- ative of ...the Sulfolobus cell cycle detected only a small increase in transcription of the DNA polymerase I prior to chromosome replication, supporting this ...
11
Genetic Requirements for the Function of the Archaeal Virus SSV1 in Sulfolobus solfataricus: Construction and Testing of Viral Shuttle Vectors
... thermophilic archaeon Sulfolobus solfataricus allowed the identification of otherwise cryptic crucial and noncrucial viral open reading frames in the genome of the archaeal virus ...
10
Formation of a Viral Replication Focus in Sulfolobus Cells Infected by the Rudivirus Sulfolobus islandicus Rod-Shaped Virus 2
... ABSTRACT Viral factories are compartmentalized centers for viral replication and assembly in infected eukaryotic cells. Here, we report the formation of a replication focus by prototypical archaeal Sulfolobus ...
13
The complete genome sequence and emendation of the hyperthermophilic, obligate iron-reducing archaeon “Geoglobus ahangari” strain 234T
... first archaeon reported to use a direct contact mechanism for Fe(III) oxide reduction, relying on a single archaellum for locomotion, numerous curled extracellular appendages for attachment, and outer-surface ...
19
Particle Assembly and Ultrastructural Features Associated with Replication of the Lytic Archaeal Virus Sulfolobus Turreted Icosahedral Virus
... Lysis of S. solfataricus cells due to STIV infection appears to be quite different from lysis seen in bacterial cells due to phage infection. From microarray studies, there is no evidence that STIV interferes with the ...
7
Responses of hyperthermophilic crenarchaea to UV irradiation
... RNA from S. solfataricus P2 was extracted at time points 30, 60, 90 and 120 minutes following UV irradiation, and puri- fied using an RNeasy Kit (Qiagen) according to the manufac- turer's directions, with the ...
18
The Architecture and Chemical Stability of the Archaeal Sulfolobus Turreted Icosahedral Virus
... The capsid architecture of STIV and the crystal structure of its major capsid protein (MCP) are strikingly similar to those of the bacteriophages PRD1, Bam35, and PM2, the alga virus PBCV-1, and the mammalian adenovirus. ...
9
Distinct Physiological Roles of the Three Ferredoxins Encoded in the Hyperthermophilic Archaeon Thermococcus kodakarensis
... Redox reactions and electron flux are critical to all metabolisms. Fds—an ancient class of Fe-S-containing small proteins—are the most widely distributed and prevalent proteinaceous electron carriers (20, 21). Almost all ...
17
Functional Genomics Investigation of Microbial Physiology in the Hyperthermophilic Microorganisms Pyrococcus furiosus and Thermotoga maritima
... Full genome cDNA microarrays were used to assess differential gene expression patterns in the hyperthermophilic archaeon Pyrococcus furiosus grown on a range of glucose- based carbohydr[r] ...
230
Life Cycle Characterization of Sulfolobus Monocaudavirus 1, an Extremophilic Spindle-Shaped Virus with Extracellular Tail Development
... growing Sulfolobus cells (28), with a majority of the cells contain- ing 2 ...a Sulfolobus culture infected with the lytic virus SIRV2 (10) exhibited a high level of cell lysis ...
7
N-terminal domain replacement changes an archaeal monoacylglycerol lipase into a triacylglycerol lipase
... [26], Sulfolobus solfataricus [27, 28], Thermococcus kodakarensis KOD1 [29], Archaeglo- bus fulgidus DSM4304 [30, 31], Pyrobaculum ...and Sulfolobus acidophilus DSM10332 ...
11
Genetic Analysis of the Major Capsid Protein of the Archaeal Fusellovirus SSV1: Mutational Flexibility and Conformational Change
... archaeal translation [28] but there is an in-frame ATG codon 18 nucleotides upstream of this TTG that is 9 nucleotides downstream of the transcriptional start site , a normal distance for translational initiation [29]. ...
25
Modeling and structural analysis of PA clan serine proteases
... 1 archaeon (Pyrococcus furiosus), 1 fungus (Neu- rospora crassa) and 1 plant (Arabidopsis thaliana) were obtained from the MEROPS protease database (http:// ...
11