Argonaute protein
Investigation of the argonaute protein variants in Toxoplasma gondii and the contribution of argonaute to RNA silencing
... of argonaute that is shorter than most other ...of argonaute was conserved as long as the putative PIWI domain was intact (Musiyenko et ...archeal argonaute protein from Archaeoglobus fulgidus ...
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Characterization of a recombinant thermotolerant argonaute protein as an endonuclease by broad guide utilization
... The argonaute protein from archaeon Methanocaldococcus fervens (MfAgo) was cloned and overexpressed in Escherichia coli BL21 ...recombinant protein showed the expected molecular weight of ~ ...
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TbAGO1, an Argonaute protein required for RNA interference, is involved in mitosis and chromosome segregation in Trypanosoma brucei
... exogenous (in vitro synthesised) dsRNA or by endogenous (expressed hairpin) dsRNA, TbAGO1-/- mutant cells are viable, showing that RNAi activity is dispensable for pro- cyclic trypanosomes in culture. Similarly, several ...
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Original Article Evaluation of Argonaute protein as a predictive marker for human clear cell renal cell carcinoma
... Abstract: Argonaute subfamily proteins are involved in human organ growth and ...the Argonaute proteins were assessed by im- munohistochemistry (IHC) in tissue microarrays (TMA), containing paired tumor ...
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A host factor supports retrotransposition of the TRE5-A population in Dictyostelium cells by suppressing an Argonaute protein
... and share a common ancestor, but only TRE5-A was amplified to a high copy number rather late in the evolu- tion of this species [12]. It is puzzling that TRE5-A amp- lification in D. discoideum was apparently accelerated ...
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Weinmann, Lasse (2009): Functional characterisation of microRNA-containing Argonaute protein complexes. Dissertation, LMU München: Fakultät für Biologie
... were detected by nuclear microinjection of si7SK1 together with WGA. The drop of loaded EGFP-Ago2 with si7SK1 in the cytoplasm results from reduced siRNA levels in the cytoplasm mediated by WGA. The preferential ...
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Transcript and metabolite analysis in Trincadeira cultivar reveals novel information regarding the dynamics of grape ripening
... Three genes coding for chromatin-remodeling pro- teins (VVTU32711_at, VVTU11309_at, VVTU38460_at) displayed different expression profiles and tend to be more expressed in 2008 season (Table 2, Additional file 6). In ...
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Original Article Argonaute 2 is up-regulated in tissues of urothelial carcinoma of bladder
... the Argonaute protein has revealed a third functionally important domain which located between the former two domains, called the MID domain which can bind the char- acteristic 5’ phosphates of small RNAs, ...
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High throughput sequencing of RNAs isolated by cross linking immunoprecipitation (HITS CLIP) reveals Argonaute associated microRNAs and targets in Schistosoma japonicum
... In recent years, microRNAs (miRNAs) have received huge attention as key regulators of gene expression both at transcriptional and post-transcriptional levels in vari- ous organisms [8–14]. miRNAs belong to a class of ...
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Ender, Christine (2009): Characterization of protein and small RNA components of Argonaute complexes. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... To perform their effector functions, miRNAs and siRNAs are incorporated into Argonaute proteins which are the key components of ribonucleoprotein complexes (miRNPs), often referred to as RNA induced silencing ...
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Mammalian Argonaute-DNA binding?
... prokaryotic Argonaute proteins are able to bind DNA and to carry out DNA-guided as well as RNA- guided mediated cleavage of DNA molecules (“DNA interference”) ...eukaryotic Argonaute proteins only interact ...
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Argonaute 2 promotes myeloma angiogenesis via microRNA dysregulation
... Drosha and Dicer are 2 key RNase III enzymes that process pre-miRNAs into mature miRNAs, which are then incorporated into the RNA-induced silencing complex (RISC) to downregulate target gene activity by triggering either ...
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RNA Silencing Gene Truncation in the Filamentous Fungus Aspergillus nidulans
... The evolutionary forces driving eukaryotic RNA silencing gene change are unclear. The data presented here indicate that Aspergillus fungi are well suited for elucidating these forces. The model fungus A. nidulans is ...
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Analysis and characterization of the genes encoding the Dicer and Argonaute proteins of Schistosoma japonicum
... acid protein. Like SmDicer, SjDicer protein contains all domains that are characteristic of metazoan dicers including an amino terminal helicase domain, DUF283, a PAZ domain, two RNAse III domains and an ...
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The Nuclear Argonaute NRDE-3 Contributes to Transitive RNAi in Caenorhabditis elegans
... We show that the nuclear RNAi Argonaute nrde-3 contrib- utes broadly and potently to RNAi triggered by exogenous dsRNA. Specifically, we found that nuclear RNAi contributes to silencing of genes that are members of ...
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Inhibition of tumourigenicity of small cell lung cancer cells by suppressing Id3 expression.
... and protein kinase C, and Id2 can be activated when bind to ATF-like transcription factors in muscle C2 cells ...CAAT/enhancer-binding protein β (C/EBPβ) complexes ...
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The growing catalog of small RNAs and their association with distinct Argonaute/Piwi family members
... Ago protein sequence variation (see Table 1) at or near the active site, as well as on the degree of mismatches between the miRNA and target RNA (Jackson et ...
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Review of the RNA interference pathway in molluscs including some possibilities for use in bivalves in aquaculture
... shock protein 60 which affects the myocardium [21]; miR-34 inhibits SIRT1 which affects the cell cycle and apoptosis [22]; miR-133 negative regulates many signals including K + channels [21]; miR-125 down ...
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Toxoplasma gondii Differentiation: Effects of Modifying Lactate Dehydrogenase and Argonaute Expression Patterns
... instructions. Cell lysates were denatured in 5X SDS protein loading dye, resolved on a 12% SDS-PAGE at 120 V for 1 h in a 1X SDS-PAGE running buffer (25 mM Tris-base (pH 8.3), 250 mM glycine, and 0.1% SDS) using ...
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Hypoxia responsive miRNAs target argonaute 1 to promote angiogenesis
... Despite a general repression of translation under hypoxia, cells selectively upregulate a set of hypoxia-inducible genes. Results from deep sequencing revealed that Let-7 and miR-103/107 are hypoxia-responsive microRNAs ...
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