ATP citrate lyase
RIPK3 promotes kidney fibrosis via AKT-dependent ATP citrate lyase
... TGF-β1 can activate the serine/threonine kinase AKT by inducing its phosphorylation in fibroblasts (10). AKT regulates many cellular processes, including cell survival, proliferation, and metabolism (11, 12). AKT ...
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Metabolic engineering with ATP-citrate lyase and nitrogen source supplementation improves itaconic acid production in Aspergillus niger
... Cells when required) were transformed by heat shock according to the manufacturer’s instructions. E. coli was grown in lysogeny broth (LB) at 37 °C. Plasmid DNA was Fig. 2 Cytosolic acetyl-CoA synthesis pathways ...
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Activation of ATP Citrate Lyase by mTOR Signal Induces Disturbed Lipid Metabolism in Hepatitis B Virus Pre-S2 Mutant Tumorigenesis
... The development of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) has been found to be associated with dis- turbed lipid metabolism. To elucidate the role of lipid metabolism in HBV tumorigenesis, we ...
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mTOR complex-2 stimulates acetyl-CoA and de novo lipogenesis through ATP citrate lyase in HER2/PIK3CA-hyperactive breast cancer
... in ATP citrate lyase (ACL) occurs frequently in human breast tumors and correlates well with HER2+ and/or PIK3CA-mutant (HER2+/ PIK3CAmut) status in breast tumor cell ...
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miR-22 inhibits tumor growth and metastasis by targeting ATP citrate lyase: evidence in osteosarcoma, prostate cancer, cervical cancer and lung cancer
... biology. ATP citrate lyase (ACLY), a key enzyme initiating de novo lipid synthesis, has been found to be upregulated in cancer cells, and its inhibition causes suppressive effects in a variety of ...
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Isolation of 5' regulatory sequences for ruminant ATP citrate lyase : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University
... ATP citrate lyase is an essential enzyme in the pathway for conversion of glucose to fatty acids in mammalian ...cytosolic citrate to acetyl CoA and oxaloacetate in an ATP-dependent ...
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ATP citrate lyase mediated cytosolic acetyl-CoA biosynthesis increases mevalonate production in Saccharomyces cerevisiae
... Background: With increasing concern about the environmental impact of a petroleum based economy, focus has shifted towards greener production strategies including metabolic engineering of microbes for the conversion of ...
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Isolation of 5' regulatory sequences for ruminant ATP citrate lyase : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University
... A diagnostic digest was carried out on the 282 bp PCR product to confirm its identity. Two restriction endonucleases, Apa I and A va I, were used to digest the PCR product. These enzymes have a single restriction site ...
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Isolation and characterisation of the 5' region sequence for the bovine ATP citrate lyase gene : a thesis presented in fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University
... ATP-citrate lyase (ACL ) is one of the major lipogenic enzymes. It catalyzes the synthesis of acetyl-CoA from citrate in the cytosol. This is the first committed step towards the conversion of ...
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The regulation of bovine ATP citrate lyase promoter : a thesis submitted in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry, Massey University, 2006
... To investigate the molecular regulation of the bovine ATP citrate lyase gene, several experimental methods were used in this study such as reporter gene assays and electrophoretic mobili[r] ...
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Functional polymorphisms of ATP citrate lyase gene predicts clinical outcome of patients with advanced colorectal cancer
... cells, citrate generated by the tricarboxylic acid cycle is preferentially exported from the mitochondria to the cytosol and then cleaved by ATP citrate lyase (ACLY) to produce cytosolic ...
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ATP-Citrate Lyase Is Required for Production of Cytosolic Acetyl Coenzyme A and Development in Aspergillus nidulans
... from citrate via ATP-citrate ...of ATP-citrate lyase, and we have deleted these ...of ATP-citrate lyase results in severe developmental effects, with the ...
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The regulation of bovine ATP citrate lyase promoter : a thesis submitted in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry, Massey University, 2006
... The presence of multiple GC box sequence elements within the 5' regulatory region of the ACLY promoter sequence suggests that Sp 1 is one of the most important transcription factors in t[r] ...
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Isolation and characterisation of the 5' region sequence for the bovine ATP citrate lyase gene : a thesis presented in fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University
... of citrate, which carries acetyl groups across the i nner mitochondrial ...membrane. Citrate is formed in the m itochondrial matrix by the condensation of acetyl-CoA with ...levels, citrate i s ...
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The central role of acetyl CoA in plant metabolism, as examined through studies of ATP citrate lyase and the bio1 mutant of Arabidopsis
... ubiquitin-dependent protein catabolism biol B-bio10 N-terminal protein myristoylation bid B-bio10 protein amino acid phosphorylation protein amino acid phosphorylation protein amino acid[r] ...
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The purification and immunological isolation of ATP citrate lyase from rat liver : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University, New Zealand
... CHAPTER 7 INTRAPORTAL LABELLING OF LIVER PROTEINS IN VIVO 7.1 INTRODUCTION 7.2 MATERIALS 7.3 METHODS 7.3.1 Intraperitoneal 24 hour labelling 7.3.2 Immunoprecipitation of ATPCL 7.4 IMMUNO[r] ...
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The purification and immunological isolation of ATP citrate lyase from rat liver : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University, New Zealand
... The lack of radioactivity in immunoreactive ATPCL subunit in rat liver which has been refed a high carbohydrate diet for 6 weeks Induced 4 in Table 23 is not due to experimental error bu[r] ...
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Adenosine triphosphate citrate lyase mediates hypocitraturia in rats
... cortical ATP cit- rate lyase protein ...in ATP citrate lyase protein abundance could explain the effect of acid feeding on ATP citrate lyase activity, we performed ...
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“Effects of Camellia sinensis and Garcinia cambogia on Obesity and its Comorbidities – Safer Alternative than Synthetic Drugs” by Ilma kauser, Syed Zaid naqvi, Rahul mathur, Nidha amir, Gunjan Sharma, Sumeet Gullaiya, Shyam sundar Agarwal, India.
... Its main component is hydroxycitric acid (HCA) which not only inhibits ATP-citrate lyase, the enzyme response for de novo fatty acid synthesis, but also increases[r] ...
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Assembling a plug and play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli
... Abbreviations: ACP, acyl carrier protein; ACL, ATP citrate lyase; BIQ, benzoisochromanequinone; BGC, biosynthesis gene cluster; CLF, chain length factor; CoA, Coenzyme A; COSY, correlati[r] ...
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