Autophagic Flux
Autophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments
... of autophagic flux affected the protective effect of GO against PrP (106–126)-mediated mitochondrial neurotoxicity (Figure ...the autophagic flux inhibitor chloroquine (CQ, 10 µ M, 12 h), and ...
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Autophagic flux is highly active in early mitosis and differentially regulated throughout the cell cycle
... is also inhibited in late G2 phase, therefore the MTOR- induced autophagy is less likely to be the major reason that differentiates the autophagic flux difference between late G2 phase and early mitosis. In ...
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Autophagic flux blockage by accumulation of weakly basic tenovins leads to elimination of B Raf mutant tumour cells that survive vemurafenib
... Autophagy is the process of self-ingestion of defective macromolecules and organelles. It provides an efficient mechanism for detoxification as well as a means for survival during nutri- ent starvation [17]. There are ...
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Impaired autophagic flux mediates acinar cell vacuole formation and trypsinogen activation in rodent models of acute pancreatitis
... Autophagy (more precisely, macroautophagy) is a sequential process through which cells degrade long-lived proteins and cytoplasmic organelles, providing their constituents for recycling (1–3). Organ- elles destined for ...
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Follicular lymphoma–associated mutations in vacuolar ATPase ATP6V1B2 activate autophagic flux and mTOR
... In this study, we examined the effect of mutations in the ATP6V1B2 subunit of the v-ATPase on mammalian cell physiolo- gy. In addition, we took advantage of the conserved nature of the v-ATPase and generated a similar ...
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Sirt1 regulates acrosome biogenesis by modulating autophagic flux during spermiogenesis in mice
... Atg7 and LC3b can be deacetylated by Sirt1 (Lee et al., 2008); the deacetylation of LC3 by Sirt1 in the nucleus is a prerequisite for LC3 redistribution to the cytoplasm, and the nucleocytoplasmic transport of LC3 is ...
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Knockdown ATG4C inhibits gliomas progression and promotes temozolomide chemosensitivity by suppressing autophagic flux
... an autophagic flux assay was performed in ATG4C stable knockdown ...agic flux can be evaluated by LC3-II protein levels in the presence or absence of inhibitors of lysosomal degradation such as ...
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Cannabidiol Attenuates Palmitic Acid-Induced Injury in Cultured Hepatocytes Through Promoting Autophagic Flux
... CQ). Autophagic flux was evaluated by Western blot ...the autophagic flux was evaluated by Western blot ...autopagic flux (evidenced by increased ratio of LC3-II/LC3-I and protein ...
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Visualizing Autophagic Flux during Endothelial Injury with a Pathway-Inspired Tandem-Reaction Based Fluorogenic Probe
... the autophagic flux both in vitro and in ...the autophagic flux during endothelial injury in both live cells and mice with high spatiotemporal ...
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Activation of autophagic flux by epigallocatechin gallate mitigates TRAIL-induced tumor cell apoptosis via down- regulation of death receptors
... activated autophagic flux and markedly decreased the protein levels of death receptors DR4 and DR5 (Figure 2 and Figure ...of autophagic flux, we used pharmacological (chloroquine) or genetic ...
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Dengue Virus Inhibition of Autophagic Flux and Dependency of Viral Replication on Proteasomal Degradation of the Autophagy Receptor p62
... basal flux under full medium conditions to the induction of autophagic flux by either nutrient deprivation or treatment with the specific mTOR inhibitor Torin1 ...quantify autophagic ...
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Autophagic Flux is Regulated by Interaction Between the C-terminal Domain of Patched1 and ATG101
... the autophagic flux under amino acid-starvation of mouse embryonic fibroblasts (MEFs) isolated from Ptc1 -/- mice or Ptc1 +/- ...reduces autophagic flux by reducing autolysosome formation or ...
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Epstein-Barr Virus Blocks the Autophagic Flux and Appropriates the Autophagic Machinery To Enhance Viral Replication
... early autophagic phases are involved in the EBV lytic replicative cycle, promoting EBV lytic gene expression and viral ...late autophagic steps do not seem to play an essential role in EBV replication, ...
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Mutations in LRRK2 potentiate age-related impairment of autophagic flux
... lgg-1 levels by fluorescence intensity (Figs. 1, 2, 3, 4 and 5), immunoblotting (Fig. 1, 3 and 5) and finally by counting puncta. Quantification of the strength of dat-1 promoter activity over the lifespan showed a ...
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Mycobacterium marinum antagonistically induces an autophagic response while repressing the autophagic flux in a TORC1- and ESX-1-dependent manner.
... the autophagic flux, as already suggested by the lack of effect of AR-12 on the frequency of GFP-Atg8 + compartments fully enclosing bacteria (Fig ...and autophagic features of the MCVs containing ...
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Neuroprotective effect of cellular prion protein (PrPC) is related with activation of alpha7 nicotinic acetylcholine receptor (α7nAchR)-mediated autophagy flux
... Also, autophagic flux is inhibited, the level of p62, an ubiquitin- and LC3-binding protein, is accumulated [34, ...of autophagic flux is ...activating autophagic flux [45-47]. ...
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Identification of the Stress Which Causes Optineurin Aggregation
... and autophagic flux ...and autophagic flux are involved in OPTN ...impaired autophagic flux induce OPTN aggregation, suggesting that OPTN aggregation might be an important the- ...
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MicroRNA-126 induces autophagy by altering cell metabolism in malignant mesothelioma
... autophagy flux or blocks autophagy initiation (this could also reduce the LC3II levels), we investigated LC3 turnover in the presence of autophagy ...or autophagic degradation (autophagosome-lysosome ...
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Therapeutic potential of autophagy-enhancing agents in Parkinson’s disease
... of autophagic degradation activity (autophagic flux) by rapamycin and lithium, po- tent autophagy enhancers routinely used in a laboratory setting, resulted in increased clearance of α-syn and neu- ...
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Tet methylcytosine dioxygenase 2 inhibits atherosclerosis via upregulation of autophagy in ApoE mice
... the autophagic process by regulating the generation of phosphatidylinositol3- phosphate and the subsequent recruitment of additional Atg proteins that orchestrate autophagosome formation ...and autophagic ...
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