avidin-biotin
Original Article Preparation of arginine-glycine-aspartic acid (RGDS)-Urokinase-carrying targeting ultrasound contrast agent by avidin-biotin system and its impacts on thrombus-targeting affinity
... Abstract: Objective: This study aimed to prepare the arginine-glycine-aspartic acid (RGDS)-urokinase-carrying target- ing ultrasound contrast agent using the avidin-biotin system (BAS) and to investigate ...
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Recombinant antigen based avidin biotin microtiter enzyme linked immunosorbent assay for serodiagnosis of invasive amebiasis
... An immunoscreening approach was used to isolate a strongly positive cDNA clone from an Entamoeba histo- lytica HK-9 cDNA expression library in the phage vector l ZAP-II. The 1.85-kb cDNA insert was found to be truncated ...
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Diagnosis of invasive candidiasis by detection of mannan antigen by using the avidin biotin enzyme immunoassay
... The diagnosis of invasive candidiasis was attempted by detection of circulating mannan antigen by using an avidin-biotin-amplified enzyme-linked immunosorbent assay AB-ELISA, and this me[r] ...
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Endogenous avidin biotin activity (EABA) in thyroid pathology: immunohistochemical study
... main biotin rich fractions are the cytosol and the mitochondria, there is some controversy as to whether moderate or negligible amounts of biotin are present in the nuclei of cells as ...well. Biotin ...
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Original Article High Endogenous Avidin Binding Activity: An Inexpensive and Readily Available Marker for the Differential Diagnosis of Kidney Neoplasms
... Three TMA slides were studied for EABA in renal neoplasms. BioCare Bull's Eye buffer (BioCare Medical, Concord, CA) with pressure cooking for 3 minutes was used for antigen retrieval. No target-specific primary antibody ...
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Neurodegeneration and contralateral α-synuclein induction after intracerebral α-synuclein injections in the anterior olfactory nucleus of a Parkinson’s disease A53T mouse model
... ABC: Avidin-biotin complex; ac: Anterior commissure; aci: Anterior commissure intrabulbar; ACo: Anterior cortical amygdala; AOD: Anterior olfactory area dorsal part; AOL: Anterior olfact[r] ...
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A Conceptual Review on Micro Bubbles
... cells, Avidin-Biotin Technology, genetically engineered molecular antibodies for human therapy, peptide radiopharmaceuticals, non- viral gene therapy has shown some good responses as far as brain drug ...
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Gene expression in macrophage rich human atherosclerotic lesions 15 lipoxygenase and acetyl low density lipoprotein receptor messenger RNA colocalize with oxidation specific lipid protein adducts
... Immunostaining of serial sections avidin-biotin-horseradish peroxidase system: A antibody specific for human macrophages HAM-56; 1:1,000 dilution; B antibody specific for human smooth mu[r] ...
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Evidence for direct estrogen regulation of the human gonadotropin releasing hormone gene
... an avidin-biotin complex DNA-binding assay demonstrated that a biotinylated DNA fragment containing -541 to -517 bp of the human GnRH gene bound 35S-labeled estrogen receptor as well as a biotinylated DNA ...
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Amine Functionalization of Bacterial Cellulose for Targeted Delivery Applications
... while avidin-HRP was used to demonstrate the ability of maximizing protein ...An avidin-biotin glucose oxidase and avidin-biotin β- galactosidase complex was conjugated to aminated ...
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Role of microglial amylin receptors in mediating beta amyloid (Aβ)-induced inflammation
... For human brain immunohistochemical staining, 20- μm-thick brain sections were cut from 1-cm-thick cor- onal block of the human inferior temporal gyrus (IFG) of an AD brain (Braak Stage 6) obtained from the Netherlands ...
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The ethanolic extract of Aralia continentalis ameliorates cognitive deficits via modifications of BDNF expression and anti inflammatory effects in a rat model of post traumatic stress disorder
... with avidin-biotin-peroxidase complex (Vector Laboratories) for 1 h at room temperature and then in a solution containing 3,3′-diaminobenzidine (DAB; Sigma- Aldrich) and ...
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AIDS Vaccination Studies Using an Ex Vivo Feline Immunodeficiency Virus Model: Homologous Erythrocytes as a Delivery System for Preferential Immunization with Putative Protective Antigens
... gently disrupted by using a detergent, and the biotinylated proteins obtained were selectively bound to avidin-biotin- coated RBC, which were then used to inoculate cats immedi- ately after coating. The ...
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Improved Methods for Immunoassay of Mycothiol
... first avidin/biotin-based immunoassay tested was a bi- otin-capture ELISA based on the capture of MS-MPB on an avidin-coated microtiter plate and detection of bound MS- MPB by anti-MSH antibody as ...
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Clear cell variant of diffuse large B cell lymphoma: a case report
... Abbreviations ABC: avidin-biotin complex; ABC-DLBCL: activated B-cell-like DLBCL; Bcl: B-cell lymphoma; BL: Burkitt lymphoma; CD: cluster designation marker; cHL: classical Hodgkin’s lym[r] ...
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N CAM is not required for initiation of secondary chondrogenesis: the role of N CAM in skeletal condensation and differentiation
... The avidin-biotin-peroxidase complex (ABC) immunohistochemistry was modified from Hsu et ...the avidin-biotin-peroxidase complex (VECTASTAIN elite ABC kit, Vector Laboratories, Buringame, CA) ...
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Effect of long-term oral administration of extra thyroxine on oviductal expression of carbonic anhydrase and avidin-related protein-2 genes in broiler breeder hens
... to biotin with high affinity (Laitinen et al., 2002). Avidin is a four-component pro- tein with 128 amino acids in each section that forms strong non-covalent bond with a biotin molecule (Ku- ramitz ...
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A Multifunctional Single-Attachment-Point Reagent for Controlled Protein Biotinylation
... Fl-Biotin-NHS was employed to prepare biotinylated versions of the monoclonal antibody cetuximab where the number of biotins varied from 0.1 to 2.5 per mole of antibody. Absorbance and fluorescence standard curves ...
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Investigations of the last step in biotin biosynthesis, the conversion of dethiobiotin to biotin
... Protein was eluted with a linear gradient of 0 to 100 % of buffer B, fractions containing DTBS were pooled, concentrated concentrated by ultafiltration using an Amicon pressure cell with[r] ...
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The Atypical Occurrence of Two Biotin Protein Ligases in Francisella novicida Is Due to Distinct Roles in Virulence and Biotin Metabolism
... Consistent with its annotation, this protein had the characteristic and atypical SDS gel mobility first seen for E. coli AccB (13). Al- though according to amino acid sequencing the E. coli AccB pro- tein is 16.7 kDa, it ...
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