Babesia bigemina
Identification of a putative methyltransferase gene of Babesia bigemina as a novel molecular biomarker uniquely expressed in parasite tick stages
... species. Babesia bigemina and ...B. bigemina develop high fever, lethargy, severe anemia, and ...[1]. Babesia bige- mina is more prevalent than other Babesia parasites be- cause it is ...
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Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle
... A competitive enzyme-linked immunosorbent assay (cELISA) based on a broadly conserved, species-specific, B-cell epitope within the C terminus of Babesia bigemina rhoptry-associated protein 1a was validated ...
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Cloning, Characterization, and Expression of a 200 Kilodalton Diagnostic Antigen of Babesia bigemina
... Current serological tests for Babesia bigemina use semipurified merozoite antigens derived from infected erythrocytes. One of the major drawbacks of these tests is that antigen quality can vary from batch ...
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Hap2, a novel gene in Babesia bigemina is expressed in tick stages, and specific antibodies block zygote formation
... Results: To isolate and sequence the hap2 gene DNA from an infected bovine with Babesia bigemina was purified. The hap2 gene was amplified, cloned and sequenced. The sequences of hap2 from four ...
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Identification of interchangeable cross species function of elongation factor 1 alpha promoters in Babesia bigemina and Babesia bovis
... B. bigemina limulus coagulation factor C domain protein (ccp)-3 gene and ...B. bigemina and B. bovis parasites. In B. bigemina culture samples were collected at 8 h post-electroporation, and in ...B. ...
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Detection of Babesia bigemina infected carriers by polymerase chain reaction amplification
... Analysis of PCR products obtained with DNA prepared by procedure 1 revealed bands of the expected sizes in samples containing DNA purified from as little as 30 infected erythrocytes 0.00[r] ...
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Genetic characterization and molecular survey of Babesia bovis, Babesia bigemina and Babesia ovata in cattle, dairy cattle and yaks in China
... B. bigemina genome in cattle, and rap-1c has proven to be transcribed but not translated [39] and could thus be used as specific molecular marker ...B. bigemina isolates from China and other countries was ...
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Molecular surveillance and phylogenetic traits of Babesia bigemina and Babesia bovis in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) from Colombia
... Although, the Babesia spp. infection is mainly associ- ated with tick exposure [2, 55], its dynamics is complex and depends on many factors, whose association deter- mines the prevalence of infection in the ...
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Identification of proteins expressed by Babesia bigemina kinetes
... B. bigemina blood stages, such as Rhoptry Associated Protein-1a (RAP-1a), can be detected by IFA throughout the entire life-cycle of the ...the Babesia KSPs described hereby are also sub- jected to such ...
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In vitro inhibition of multiplication of Babesia bigemina by using monoclonal antibodies
... Observation with the light microscope of smears from the culture wells treated with the different ascitic fluids or purified IgGs revealed that the majority of the MAb with inhibitory ac[r] ...
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Molecular detection and characterization of Babesia bovis, Babesia bigemina, Theileria species and Anaplasma marginale isolated from cattle in Kenya
... All the cattle in this study appeared healthy, although pathogenic hemoparasites (B. bovis, B. bigemina, A. marginale and T. parva) were detected in their blood. This absence of clinical disease in infected cattle ...
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Detection of Babesia bovis and Babesia bigemina in Water Buffaloes (Bubalus bubalis) in Endemic Areas of São Paulo State, Brazil
... B. bigemina PCR amplifica- tions were: initial denaturation at 95 ˚C for 2 min; 40 cycles of 94˚C for 1 min (denaturation), 66˚C for 1 min (annealing), 72 ˚C for 1 min (extension) and a final extension at 72˚C for ...
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Pohl, Anna Elisabeth (2013): Epidemiology study of tick-borne diseases in cattle in Minas Gerais, Brazil. Dissertation, LMU München: Tierärztliche Fakultät
... parasites Babesia bovis and Babesia bigemina, is a tick-borne disease responsible for important economic losses in the cattle industry in tropical and subtropical areas of the world (Barros et ...B. ...
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Ellagic acid microspheres restrict the growth of Babesia and Theileria in vitro and Babesia microti in vivo
... of Babesia bovis, Babesia bigemina, Babesia divergens, Babesia caballi and Theileria equi in vitro and their chemotherapeutic potential on Babesia microti in ...B. ...
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Serological and molecular diagnostic surveys combined with examining hematological profiles suggests increased levels of infection and hematological response of cattle to babesiosis infections compared to native buffaloes in Egypt
... by Babesia bovis and Babesia ...B. bigemina is known to be transmitted by both ...B. bigemina and ...to Babesia infections [10], the hematological response of buffaloes to ...
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Babesia lengau sp nov , a Novel Babesia Species in Cheetah (Acinonyx jubatus, Schreber, 1775) Populations in South Africa
... between Babesia lengau sp. nov. and other Babesia and Theileria species were analyzed using both neighbor-joining and maximum parsimony phylo- genetic ...3. Babesia lengau ...the Babesia spp. ...
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Establishment of Babesia vulpes n sp (Apicomplexa: Babesiidae), a piroplasmid species pathogenic for domestic dogs
... The COX1 has been increasingly applied in molecu- lar phylogenetic studies of piroplasmids [20, 46, 47]. The phylogenetic analysis using COX1 demonstrated that B. vulpes n. sp. does not segregate into Theileria (s.s.) ...
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High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis
... Initial DNA template concentrations were found to impact Tm fluctuations, which had previously been reported [37, 38]. Once Tm values and melting curves shape are shifted, it can lead to a misidentification of spe- cific ...
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One Health approach to identify research needs in bovine and human babesioses: workshop report
... Bovine, canine, and equine babesioses are among the most economically relevant infections of domestic animals. Infestations with CFT, Rhipicephalus (Boophilus)microplus and R. (B.)annulatus, economically impact cattle ...
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The role of healthy dog carriers of Babesia microti like piroplasms
... Our data point to higher proportions of infected male than female dogs, as described for Bml in foxes in the Great Britain [23] and for B. vogeli in dogs in Italy [46]. Furthermore, we detected a higher percentage of ...
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