Babesia bovis
Genome Editing of Babesia bovis Using the CRISPR/Cas9 System
... ABSTRACT Babesia bovis, the most virulent causative agent of bovine babesiosis, is prevalent in tropical and subtropical regions of the ...B. bovis and demonstrated its potential for genome ...B. ...
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Analysis of Babesia bovis infection induced gene expression changes in larvae from the cattle tick, Rhipicephalus (Boophilus) microplus
... and Babesia bovis- infected larval samples, total RNA was isolated from a pool of several thousand tick larvae using the FastPrep-24 Tissue and Cell Homogenizer and Lysing Matrix D (Qbio- gene, Irvine, CA, ...
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Validation and Field Evaluation of a Competitive Enzyme-Linked Immunosorbent Assay for Diagnosis of Babesia bovis Infections in Argentina
... by Babesia bovis limit cattle production and cause important economic losses in tropical and subtropical areas around the ...B. bovis merozoites and sporozoites and contains B-cell epitopes that are ...
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Persistently Infected Calves as Reservoirs for Acquisition and Transovarial Transmission of Babesia bovis by Rhipicephalus (Boophilus) microplus
... Babesia bovis is a deadly disease of cattle resulting in severe economic losses in the vast regions of the world where it is endemic. If reintroduced into the United States, babesiosis would cause ...
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Monitoring Babesia bovis infections in cattle by using PCR based tests
... methods to monitor them. Thus, in an effort to improve de- tection, recent approaches have employed in vitro target am- plification. Reports from Figueroa et al. (9) and Fahrimal et al. (8) indicate that the limits of ...
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Loss of neurovirulence is associated with reduction of cerebral capillary sequestration during acute Babesia bovis infection
... Babesia bovis is a tick-borne apicomplexan parasite that infects cattle in tropical and sub-tropical regions world- ...B. bovis incapable of indu- cing neurovirulence are derived in vivo through ...
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Genetic characterization and molecular survey of Babesia bovis, Babesia bigemina and Babesia ovata in cattle, dairy cattle and yaks in China
... mainly Babesia bovis and ...B. bovis, ...described Babesia U ...B. bovis and ...B. bovis infection, which results in high parasitaemia and a more severe disease than ...of ...
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Spherical Body Protein 2 truncated copy 11 as a specific Babesia bovis attenuation marker
... B. bovis strains were reported in GenBank with accession numbers MG430176, MG430177 and MG430178, ...B. bovis by MacVector ...Texas Babesia bovis strain were performed by MacVector ...
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Bovipain 2, the falcipain 2 ortholog, is expressed in intraerythrocytic stages of the tick transmitted hemoparasite Babesia bovis
... C1 cysteine peptidases were identified from the proteome as predicted by the genome of Babesia bovis (T2Bo strain), Theileria annulata (Ankara strain) and T. parva (Mumuga strain) [38] using the MEROPS ...
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Transovarial Transmission Efficiency of Babesia bovis Tick Stages Acquired by Rhipicephalus (Boophilus) microplus during Acute Infection
... parasite Babesia bovis, a reemerging threat to ...B. bovis infection within Rhipicephalus (Boophilus) microplus following acquisition feeding on acutely parasitemic ...B. bovis during ...
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Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Babesia bovis
... A previously developed competitive enzyme-linked immunosorbent assay (cELISA) based on a species- specific, broadly conserved, and tandemly repeated B-cell epitope within the C terminus of rhoptry-associated protein 1 of ...
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Silencing expression of the Rhipicephalus microplus vitellogenin receptor gene blocks Babesia bovis transmission and interferes with oocyte maturation
... Detection of Babesia bovis transovarial transmission Whole-genomic DNA was isolated from individual un- fed R. microplus larva from the three groups. Larvae were collected, washed in 75% ethanol, and stored ...
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Natural Babesia bovis Infection in Water Buffaloes (Bubalus bubalis) and Crossbred Cattle under Field Conditions in Egypt: a Preliminary Study.
... Babesia bovis is one of the most im- portant blood parasites affecting cattle and buffaloes and in its acute forms, it lowers the productive performance of the affected ani- mals (Talkhan et ...B. ...
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Detection of Babesia bovis and Babesia bigemina in Water Buffaloes (Bubalus bubalis) in Endemic Areas of São Paulo State, Brazil
... Babesiosis is a tick-transmitted disease that causes severe economic losses to the cattle industry in Brazil. Water buffaloes (Bubalus bubalis) are often carriers of Babesia spp., but there are no studies that ...
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Babesia bovis RON2 contains conserved B cell epitopes that induce an invasion blocking humoral immune response in immunized cattle
... in Babesia bovis The ...B. bovis T2Bo reference ...B. bovis and ...B. bovis T2Bo ron2 gene using Oligoanalyzer ...B. bovis, each from a different state in Mexico (Chiapas, ...
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Spherical Body Protein 4 Is a New Serological Antigen for Global Detection of Babesia bovis Infection in Cattle
... Five Babesia bovis recombinant proteins, including merozoite surface antigen 2c (BbMSA-2c), C-terminal rhoptry-associated protein 1 (BbRAP-1/CT), truncated thrombospondin-related anonymous protein ...
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Identification of interchangeable cross species function of elongation factor 1 alpha promoters in Babesia bigemina and Babesia bovis
... control Babesia parasites and its vectors [3]. Babesia bovis and ...while Babesia divergens is mainly prevalent in the European continent ...
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Competitive Enzyme-Linked Immunosorbent Assay Based on a Rhoptry-Associated Protein 1 Epitope Specifically Identifies Babesia bovis-Infected Cattle
... hemoparasite Babesia bovis was cloned and expressed as a histidine-tagged thioredoxin fusion peptide and used as antigen in a ...B. bovis-specific antibod- ies from uninfected animals and from ...
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Molecular surveillance and phylogenetic traits of Babesia bigemina and Babesia bovis in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) from Colombia
... Although buffaloes did not show clinical signs associ- ated with infection, the prevalence was variable between age groups (Fig. 3b), as previously observed in endemic areas of South America [11, 50]. In Colombia, like ...
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Geno and phenotypic characteristics of a transfected Babesia bovis 6 Cys E knockout clonal line
... The egfp-bsd fusion gene insertion in the clonal line 6- Cys EKO cln seem to be limited to the intended 6-Cys gene E, and transfection does not appear to have altered the genome of 6-Cys EKO cln at sites other than that ...
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