Bacterial Artificial Chromosome (BAC)
Application of a Bacterial Artificial Chromosome Modification System for a Human Artificial Chromosome Vector
... BAC, bacterial artificial chromosome; HPRT, hypoxanthine phosphoribosyl transferase; IL, interleukin; SLG, stable luciferase green; SLR, stable luciferase ...
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The development and characterisation of a bacterial artificial chromosome library for Fragaria vesca
... Abstract Background: The cultivated strawberry Fragaria ×ananassa is one of the most economically- important soft-fruit species. Few structural genomic resources have been reported for Fragaria and there exists an urgent ...
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Construction and Characterization of Two Bacterial Artificial Chromosome Libraries of Grass Carp
... Feng Xiong & Lanjie Liao & Yaping Wang & Zuoyan Zhu Received: 3 September 2009 / Accepted: 17 December 2009 / Published online: 26 March 2010 # Springer Science+Business Media, LLC 2010 Abstract ...
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Bacterial artificial chromosome derived simian varicella virus is pathogenic in vivo
... Conclusions: SVV BAC is as pathogenic and immunogenic as WT SVV in vivo. Thus, the SVV BAC genetic system combined with the rhesus macaque animal model can further our understanding of viral ORFs important for VZV ...
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Cloning and Mutagenesis of the Murine Gammaherpesvirus 68 Genome as an Infectious Bacterial Artificial Chromosome
... Gammaherpesviruses cause important infections of humans, in particular in immunocompromised patients. Recently, murine gammaherpesvirus 68 (MHV-68) infection of mice has been developed as a small animal model of ...
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The bacterial artificial chromosome (BAC) library of the narrow-leafed lupin (Lupinus angustifolius L.)
... 1 Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland, 2 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Sokolovská 6, CZ-772 00 Olomouc, Czech ...
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Construction and Manipulation of an Infectious Clone of the Bovine Herpesvirus 1 Genome Maintained as a Bacterial Artificial Chromosome
... The complete genome of bovine herpesvirus 1 (BoHV-1) strain V155 has been cloned as a bacterial artificial chromosome (BAC). Following electroporation into Escherichia coli strain DH10B, the BoHV-1 ...
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Construction of a full length infectious bacterial artificial chromosome clone of duck enteritis virus vaccine strain
... infectious bacterial artificial chromosome maintaining full-length genome without any deletions or destruction of the viral coding sequence, and the viruses rescued from the DEV-BAC clone exhibited ...
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Construction of a high coverage bacterial artificial chromosome library and comprehensive genetic linkage map of yellowtail Seriola quinqueradiata
... Oryza bacterial artificial chromosome library resource: construction and analysis of 12 deep-coverage large-insert BAC libraries that represent the 10 genome types of the genus ...
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Cloning of the Full-Length Rhesus Cytomegalovirus Genome as an Infectious and Self-Excisable Bacterial Artificial Chromosome for Analysis of Viral Pathogenesis
... a bacterial artificial chromosome (BAC) containing an enhanced green fluorescent protein (EGFP) cassette was engineered into the intergenic region between unique short 1 (US1) and US2 of the ...
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Functional Characterization of Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame K8 by Bacterial Artificial Chromosome-Based Mutagenesis
... Received 28 September 2010/Accepted 6 December 2010 The open reading frame K8 of Kaposi’s sarcoma-associated herpesvirus (KSHV) encodes a basic leucine zipper (bZip) protein that binds to the origin of viral DNA ...
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Construction of an Infectious Rhesus Rhadinovirus Bacterial Artificial Chromosome for the Analysis of Kaposi's Sarcoma-Associated Herpesvirus-Related Disease Development
... Immunology, Oregon Health & Science University, Portland, Oregon 97201 3 Received 13 September 2006/Accepted 28 December 2006 Rhesus rhadinovirus (RRV) is closely related to Kaposi’s sarcoma-associated herpesvirus ...
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Construction of a Self-Excisable Bacterial Artificial Chromosome Containing the Human Cytomegalovirus Genome and Mutagenesis of the Diploid TRL/IRL13 Gene
... Dong Yu, Gregory A. Smith,† Lynn W. Enquist, and Thomas Shenk* Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014 Received 12 October 2001/Accepted 5 December 2001 The full-length ...
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Construction of a Single Nucleotide Polymorphism Linkage Map for the Silkworm, Bombyx mori, Based on Bacterial Artificial Chromosome End Sequences
... We have developed a linkage map for the silkworm Bombyx mori based on single nucleotide poly- morphisms (SNPs) between strains p50T and C108T initially found on regions corresponding to the end sequences of ...
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Recovery of infectious virus from full-length cowpox virus (CPXV) DNA cloned as a bacterial artificial chromosome (BAC)
... Swaantje J Roth 1 , Dirk Höper 2 , Martin Beer 2 , Silke Feineis 1 , B Karsten Tischer 1 , Nikolaus Osterrieder 1* Abstract Transmission from pet rats and cats to humans as well as severe infection in felids and other ...
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Production of High-Titer Epstein-Barr Virus Recombinants Derived from Akata Cells by Using a Bacterial Artificial Chromosome System
... An Epstein-Barr virus (EBV) genome in Burkitt’s lymphoma-derived cell line Akata was cloned into a bacterial artificial chromosome (BAC) vector. The BAC clone, designated AK-BAC, was rapidly and ...
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Construction and Manipulation of a New Kaposi's Sarcoma-Associated Herpesvirus Bacterial Artificial Chromosome Clone
... Herpesvirus Bacterial Artificial Chromosome Clone Kevin F. Brulois, a Heesoon Chang, a,b Amy Si-Ying Lee, b Armin Ensser, a,c Lai-Yee Wong, a,b Zsolt Toth, a Sun Hwa Lee, a Hye-Ra Lee, a,b Jinjong ...
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Preliminary study of the UL55 gene based on infectious Chinese virulent duck enteritis virus bacterial artificial chromosome clone
... Preliminary study of the UL55 gene based on infectious Chinese virulent duck enteritis virus bacterial artificial chromosome clone RESEARCH Open Access Preliminary study of the UL55 gene based on infe[.] ...
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A Bacterial Artificial Chromosome-Based Genetic Linkage Map of the Nematode Pristionchus pacificus
... Manuscript received March 12, 2002 Accepted for publication May 24, 2002 ABSTRACT To understand the evolution of developmental processes, nonmodel organisms in the nematodes, insects, and vertebrates are compared with ...
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An Overlapping Bacterial Artificial Chromosome System That Generates Vectorless Progeny for Channel Catfish Herpesvirus
... Mississippi State University, Mississippi State, Mississippi 39762 Received 1 October 2007/Accepted 21 January 2008 Herpesviruses are important pathogens of humans and other animals. Herpesvirus infectious clones that ...
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