Bacterial isolates, DNA preparation and sequencing
Discordant bioinformatic predictions of antimicrobial resistance from whole-genome sequencing data of bacterial isolates: an inter-laboratory study.
... Discordant bioinformatic predictions of antimicrobial resistance from whole- genome sequencing data of bacterial isolates: an inter- laboratory study Ronan M. Doyle 1,2, *, Denise M. O'Sullivan 3 , ...
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Whole-Genome Sequencing and Concordance Between Antimicrobial Susceptibility Genotypes and Phenotypes of Bacterial Isolates Associated with Bovine Respiratory Disease.
... of bacterial pathogens associated with bovine respiratory disease, the most prevalent cause of cattle mortality in the United ...Whole-genome sequencing offers a culture- independent alternative to current ...
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16S Ribosomal DNA Sequence Analysis of a Large Collection of Environmental and Clinical Unidentifiable Bacterial Isolates
... Extracted DNA was amplified by using PCR tech- nology and the universal 16S rDNA primers fD1 and rp2 (30) (Eurogentec, Seraing, ...and sequencing of amplified products were done as previously described ...
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Systematic 16S rRNA Gene Sequencing of Atypical Clinical Isolates Identified 27 New Bacterial Species Associated with Humans
... 16 isolates belonging to species not previously recovered from patients and a total of 120 isolates that belonged to species which had been the sub- ject of fewer than 10 clinical case ...the ...
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Usefulness of the MicroSeq 500 16S Ribosomal DNA Based Bacterial Identification System for Identification of Clinically Significant Bacterial Isolates with Ambiguous Biochemical Profiles
... encountered bacterial species, and noncultivable ...of sequencing the am- plified PCR ...the DNA amplification and se- quencing steps, it would not be cost-effective to use the Micro- Seq 500 16S ...
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Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
... the DNA 12000 assay, but the instrument only runs 12 sam- ples at a time and cannot be ...of preparation of libraries with the SMRTbell Template Preparation kit as well as analysis of gDNA, ...
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Application of different DNA extraction procedures, library preparation protocols and sequencing platforms: impact on sequencing results
... for DNA extraction, two library preparation kits and two NGS Illumina sequencing ...to bacterial genome ...related bacterial strains, one should consider alignment-based and variant ...
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Pooled DNA sequencing identifies genomic regions of selection in Nigerian isolates of Plasmodium falciparum
... individual sequencing of ...High-throughput sequencing on samples pooled from different individuals is a strategy that has been adopted to characterize genetic variability in diploid Drosophila at a small ...
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Molecular Typing of Isolates of Rickettsia rickettsii by Use of DNA Sequencing of Variable Intergenic Regions
... Sandor E. Karpathy, Gregory A. Dasch, and Marina E. Eremeeva* Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333 Received 16 February 2007/Accepted 11 May 2007 Rickettsia ...
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Inherent bacterial DNA contamination of extraction and sequencing reagents may affect interpretation of microbiota in low bacterial biomass samples
... contaminating DNA from all tissue extraction control blanks and, being aerobic and a having a preferred grow- ing temperature of 28 °C, is not generally considered a normal inhabitant of the gastrointestinal ...
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Single-strand DNA library preparation improves sequencing of formalin-fixed and paraffin-embedded (FFPE) cancer DNA
... single-strand DNA library preparation method originally developed for ancient DNA to conventional protocols using double-stranded DNA derived from FFPE material we obtain on average 900-fold ...
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Illumina mate-paired DNA sequencing-library preparation using Cre-Lox recombination
... 5-kb DNA fragments by a blunt-end circularization. Sequencing reads that pass through the junction of the two joined ends of a 3–5-kb DNA fragment are not easy to identify and pose problems during ...
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An Overview of DNA Sequencing
... Library prep, amplification and sequencing: 2-4 days Single sample preparation from bacterial to human genomic DNA Single amplification per genome with no cloning or cloning artifacts Pi[r] ...
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Amplicon Template Preparation and Sequencing
... for sequencing on the instrument, refer to the RS Remote Online Help system or Pacific Biosciences Software Getting Started Guide for more ...a DNA Sequencing Kit and SMRT Cells for standard ...
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Isolation of Bacterial DNA
... this preparation gently with the sterilized glass rod so that the ethanol is very gradually mixed throughout the entire aqueous ...the DNA strands and make the DNA more difficult to isolate. The ...
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Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
... representative result is shown in Figure 3. Panel A shows the fabricated Gel-seq device; false color has been added to distinguish the different gel regions. Panel B shows a close up of four different separations used ...
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Public Health Value of Next Generation DNA Sequencing of Enterohemorrhagic Escherichia coli Isolates from an Outbreak
... 16 isolates covering the whole outbreak, we have shown that there is enough variation to develop a robust typing ...whole-genome sequencing that de- tects all SNPs and that detects insertion deletion events ...
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Twenty years of bacterial genome sequencing
... High-throughput sequencing has been applied to other areas, from the study of ancient pathogens to the analysis of single ...ancient DNA research has delivered pathogen genomes from the past, including ...
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Impact of DNA Sequencing and Analysis Methods on 16S rRNA Gene Bacterial Community Analysis of Dairy Products
... of bacterial DNA standards from nine dairy-relevant bacterial species, we found that DNA sequencing and analysis pipelines contributed significant variations to OTU/ASV distributions and ...
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CUSTOM DNA SEQUENCING SERVICES
... This service is totally customised according to your requirements. We provide individual support at every stage, and offer a wide range of options for sample preparation and sequencing reactions, selectable ...
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