baculovirus-insect cell system
Optimization of recombinant human transferrin expression in insect cells baculovirus system
... the baculovirus insect cell system has attracted wide attention as vectors for high level and faithful expression of a variety of heterologous ...
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Production and purification of VP2 protein of porcine parvovirus expressed in an insect baculovirus cell system
... a baculovirus system, although baculovirus expression systems are likely to be more costly than bacterial expression systems for producing viral proteins and may present difficulty in purifying ...
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A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System
... Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant ...a baculovirus expression vector encoding a ...
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Evaluation of an Enzyme Linked Immunosorbent Assay with Recombinant Rhoptry Associated Protein 1 Antigen against Babesia bovis for the Detection of Specific Antibodies in Cattle
... host cell invasion (21, 22). The major immunogenic B-cell and T-cell epitopes on RAP-1 are conserved among all strains tested, but they are not conserved between different species (5, ...in ...
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Baculovirus coinfection strategy for improved galactosylation of recombinant glycoprotein produced by insect cell culture
... Vector System (BEVS) is widely used for the production of recombinant glycoproteins, but it is not ideal for pharmaceutical glycoprotein production due to incomplete ...acceptor. Insect cell lines ...
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Lymantria dispar Nucleopolyhedrovirushrf-1 Expands the Larval Host Range of Autographa californica Nucleopolyhedrovirus
... in insect hemolymph indicated that Autographa californica NPV (AcNPV) nucleocapsids budded through the midgut basal lamina into the hemocoel of Tricho- plusia ni larvae within ...tracheal system, which ...
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Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
... the Baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) downstream of the strong polyhedrin ...the insect cell line, Spodoptera frugiperda (Sf9) ...
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Expression of Recombinant Small Hydrophobic Protein for Serospecific Detection of Avian Pneumovirus Subgroup C
... The baculovirus expression system has been used extensively to express large quantities of proteins that are antigenically similar to their native counterparts and can be used in stan- dardized assays to ...
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Identification of baculovirus gene that promotes Autographa californica nuclear polyhedrosis virus replication in a nonpermissive insect cell line.
... and cell- mediated ...defense system against viral infection, the shutoff of protein ...other baculovirus genes that have been implicated in host range determination, p35 (10, 11), the p143 helicase ...
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Optimization of canine interleukin 12 production using a baculovirus insect cell expression system
... To determine the quality, samples of the purified pro- tein were analyzed by SDS–PAGE, Western blot, fluo- rescence emission spectrum, circular dichroism, and for endotoxin contamination. Evaluation carried out by ...
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Expression and characterization of hepatitis B virus surface antigen polypeptides in insect cells with a baculovirus expression system.
... The expression of two S polypeptides by recombinant viruses 373S and 106FS suggested that the polypeptides represented the unglycosylated p25 and glycosylated gp29 forms of the small HBs[r] ...
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Identification and Expression of a 50 Kilodalton Surface Antigen of Babesia gibsoni and Evaluation of Its Diagnostic Potential in an Enzyme Linked Immunosorbent Assay
... by baculovirus was evalu- ated for its diagnostic potential in an ...in insect cells is sim- ilar to the native P50 protein in terms of its molecular structure and ...in insect cells is suitable for ...
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Characterisation of the adenovirus protease
... One of the substrates of the protease, the pre-terminal pTP protein was expressed in insect cells using a recombinant baculovirus.. Coinfections of cells with recombinant 23-kDa and pTP [r] ...
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Baculovirus VP1054 Is an Acquired Cellular PURα, a Nucleic Acid-Binding Protein Specific for GGN Repeats
... transcription, cell cycle regulation, and mRNA export and translation (see references 47 and 48 for ...four baculovirus genera aligned with a set of well-defined insect PUR␣ proteins are illustrated ...
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Effects of baculovirus infection on IE1-mediated foreign gene expression in stably transformed insect cells.
... IEl-mediated expression of the integrated genes in transformed insect cells is likely to be blocked at the transcriptional level at late times of baculovirus infection, when the foreign [r] ...
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Histidine at Residue 99 and the Transmembrane Region of the Precursor Membrane prM Protein Are Important for the prM-E Heterodimeric Complex Formation of Japanese Encephalitis Virus
... TEM for RSP visualization. Culture supernatants of baculovirus-infected Sf9 cells (MOI ⫽ 2.5) or JEV-infected Sf9 cells at 5 days postinfection were centri- fuged, precipitated with 10% polyethylene glycol 8000, ...
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Assembly of double-shelled, viruslike particles of bluetongue virus by the simultaneous expression of four structural proteins.
... Coexpression of the two major core proteins VP3 and VP7 in insect cells by a multiple recombinant baculovirus results in the synthesis of CLPs of the same size, appearance, and stoichiom[r] ...
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Molecular Cloning of a Babesia caballi Gene Encoding the 134-Kilodalton Protein and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay
... The enzyme-linked immunosorbent assay (ELISA) using the whole lysates of B. caballi-infected erythrocytes was found to cause an extensive cross-reaction between B. caballi- and B. equi-infected horse sera (2, 24). ...
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Recombinant virus vaccine for bluetongue disease in sheep.
... Bluetongue virus tubules made in insect cells by recombinant baculovirus: expression of NS1 gene of bluetongue virus serotype 10.. Structure of the bluetongue virus capsid.[r] ...
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Recombinant type 5 adenoviruses expressing bovine parainfluenza virus type 3 glycoproteins protect Sigmodon hispidus cotton rats from bovine parainfluenza virus type 3 infection.
... At 5 days after viral inoculation, lungs had a mottled ap- pearance upon gross examination of the tissue. Immunohisto- chemically, viral antigen was demonstrated to be widespread within the airway epithelium, and ...
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