bafilomycin A1
BAFILOMYCIN A1 AT NANOMOLAR CONCENTRATIONS SATURABLY INHIBITS A PORTION OF TURTLE BLADDER ACIDIFICATION CURRENT
... plasma membrane, and the CaATPase of sarcoplasmic reticulum (Bowman et al., 1988; Dröse et al., 1993; Dröse and Altendorf, 1997). Balifomycin only shows specificity for vacuolar ATPases at suitably low concentrations ...
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Effect of Bafilomycin A1 and Nocodazole on Endocytic Transport in HeLa Cells: Implications for Viral Uncoating and Infection
... As bafilomycin not only dissipates the low endosomal pH but also blocks transport from early to late endosomes in HeLa cells, its inhibitory effect on viral infection could in part also be attributed to trapping ...
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Progeny Varicella-Zoster Virus Capsids Exit the Nucleus but Never Undergo Secondary Envelopment during Autophagic Flux Inhibition by Bafilomycin A1
... Cells exhibit basal levels of autophagy even during herpesvirus infection (13). However, the levels of autophagy induced by VZV infection in both (i) human skin during the VZV disease called herpes zoster (shingles) and ...
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Effects of bafilomycin A1 on cytosolic pH of sheep alveolar and peritoneal macrophages: evaluation of the pH regulatory role of plasma membrane V ATPases
... the bafilomycin-sensitivity of resident sheep m f with that of thioglycolate-elicited murine m f is complicated by ...the bafilomycin-sensitivity of m f pHi, it is clear that one must look beyond ...
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Two Distinct Low-pH Steps Promote Entry of Vaccinia Virus
... route. Bafilomycin A1 and monensin, inhibitors of endosomal acidi- fication, were used to determine whether this was ...that bafilomycin A1 and monensin reduced the entry of control MVs by 70 ...
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Cell-Type-Specific Characteristics Modulate the Transduction Efficiency of Adeno-Associated Virus Type 2 and Restrain Infection of Endothelial Cells
... Adeno-associated viruses (AAVs) are promising vectors for various gene therapy applications due to their long-lasting transgene expression and wide spectrum of target cells. Recently, however, it has become apparent that ...
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Endoplasmic reticulum stress triggers Xanthoangelol-induced protective autophagy via activation of JNK/c-Jun Axis in hepatocellular carcinoma
... 3-MA, Bafilomycin A1 (Baf A1), or siRNA targeting Atg5 effectively enhanced the apoptotic cell ratio in XAG-treated cells, indicating that protective effect of autophagy induced by XAG in ...
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Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry
... exact molecular mechanism for the inhibitory effect of the latter two methods is not known, but it can be expected that also other cellular functions are affected.  -MCD has been shown to block endocytosis by depleting ...
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Comparison of gene transfection and cytotoxicity mechanisms of linear poly(amidoamine) and branched poly(ethyleneimine) polyplexes
... Fig.8: Effect of bafilomycin A1 500 nM on A the metabolic activity and B the transfection efficiency measured by a Presto Blue or luciferase assay, respectively 20h following exposure of[r] ...
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An investigation of NAADP dependent Ca²⁺ signalling mechanisms in arterial smooth muscle
... to Bafilomycin A1 within these cells. Thus, Bafilomycin A1 did not deplete the NAADP-sensitive Ca 2+ store in sea urchin eggs, as application of NAADP induced a robust Ca 2+ signal in the ...
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ER stress–mediated autophagy promotes Myc dependent transformation and tumor growth
... To determine whether autophagy induction actively protects cells from c-Myc–induced apoptosis, we blocked autophagy in mycER:Perkfl/fl MEFs by treatment with bafilomycin A1 and measured [r] ...
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Separate Cellular Localizations of Human T-Lymphotropic Virus 1 (HTLV-1) Env and Glucose Transporter Type 1 (GLUT1) Are Required for HTLV-1 Env-Mediated Fusion and Infection
... by bafilomycin A1 (BFLA1) treatment in 293T cells, HTLV-1 Env-mediated cell fusion and infection also were inhibited without the overexpression of GLUT1, indicating that the localization of GLUT1 in ...
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Characterization of Lassa Virus Cell Entry and Neutralization with Lassa Virus Pseudoparticles
... with bafilomycin A1 prior to and during infection inhibited infection with wt LV (data not ...Consistently, bafilomycin A1 also inhibited, in a dose-dependent fashion, cell entry of LVpp and ...
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Hepatitis C Virus Entry Requires a Critical Postinternalization Step and Delivery to Early Endosomes via Clathrin-Coated Vesicles
... mediated membrane fusion. We cannot completely exclude the possibility that conformational changes and protein dissocia- tion kinetics leading up to fusion are exceptionally slow for HCV E1E2 and require the continuous ...
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Paclitaxel and the dietary flavonoid fisetin: a synergistic combination that induces mitotic catastrophe and autophagic cell death in A549 non-small cell lung cancer cells
... nM bafilomycin A1 (Baf A1) for 4 h, followed by washing with PBS and subse- quent incubation in the absence or presence of the tested compounds for the indicated period of ...
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Dysregulation of autophagy in chronic lymphocytic leukemia with the small molecule Sirtuin inhibitor Tenovin 6
... Adding bafilomycin-A1, an autophagy inhibitor to Tnv-6 containing cultures did not cause synergistic accumulation of LC3-II, suggesting inhibition of late-stage autophagy by ...
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Molecular Interactions of Autophagy with the Immune System and Cancer
... As previously mentioned, inhibition of autophagy encourages cancer cell growth; promotion of autophagy can be a cancer suppressor at early-stage cancer [37, 51]. At the late stage of cancer, however, cancer cells utilize ...
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Human Rhinovirus Type 2 Uncoating at the Plasma Membrane Is Not Affected by a pH Gradient but Is Affected by the Membrane Potential
... without bafilomycin in MEM, washed, and transferred into infection medium with or without bafilomycin for incubation with HRV2 at 4°C; after 1 h, nonattached virus was washed away and the cells were ...
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Risk assessment of LNG and FLNG vessels during manoeuvring in open sea
... D&4..2&5ÿ?@ÿAÿEBÿ ÿCÿ A1;JIKBÿHCÿ A1;JIKBÿICÿ A1;JIKBÿMCÿ A1;JIKBÿJCÿ A1;JIKBÿKCÿ A1;JIKBÿ0Cÿ A1;JIKBÿNCÿ A1;JIKBÿPCÿ A1;JIKBÿHCÿ A1;JIKBÿICÿ A1;JIKBÿMCÿ A1;JIKBÿJCÿ A1;JIKBÿKCÿ... Q5ÿrÿ[r] ...
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AN INVESTIGATION OF THE MIDGUT K+ PUMP OF THE TOBACCO HORNWORM (MANDUCA SEXTA) USING SPECIFIC INHIBITORS AND AMPHOTERICIN B
... relatively high doses of bafilomycin and amiloride, but also by NBD-Cl (not shown here), was always incomplete and seemed to be non-specific in the case of NBD-Cl. Hence, we cannot yet exclude the possibility ...
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