BCA: bicinchoninic acid
ZiBu PiYin recipe prevents diabetes associated cognitive decline in rats: possible involvement of ameliorating mitochondrial dysfunction, insulin resistance pathway and histopathological changes
... B; BCA, bicinchoninic acid; CNS, central nervous system; DACD, diabetes associated cognitive decline; FSI, fasting serum insulin; IOD, integrated optical density; IRS, Insulin receptor substrate; ...
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Quantitative proteomics analysis of vitreous body from type 2 diabetic patients with proliferative diabetic retinopathy
... H; BCA: Bicinchoninic acid; C2: Complement C2; C4BPA: C4b-binding protein alpha chain; C4BPA: Complement component 4 binding protein alpha; CA1: Carbonic anhydrase 1; CAT: Catalase; CFD: Complement ...
12
Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta
... tissue; BCA: Bicinchoninic acid; bFGF: Basic fibroblast growth factor; BM: Bone marrow; cDNA: Complementary deoxyribonucleic acid; CSHL: Chorionic somatomammotropin hormone Like 1; CV: ...
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Human liver stem cells attenuate concanavalin A-induced acute liver injury by modulating myeloid-derived suppressor cells and CD4+ T cells in mice
... aminotransferase; BCA: Bicinchoninic acid; CK: Cytokeratin; ConA: Concanavalin A; DMEM: Dulbecco ’ s modified Eagle medium; DMSO: Dimethyl sulfoxide; ECL: Enhanced chemiluminescence; ELISA: ...
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An Ultrasensitive Gold Nanoparticle based Lateral Flow Test for the Detection of Active Botulinum Neurotoxin Type A
... until its pH value reached 3.4. The medium was kept at 4 °C overnight and then centrifuged at 12,000 × g for 30 min. The supernatant was discarded, and the precipi- tates were dissolved with 0.2 M PBS (pH 6.0) under ...
6
SGIP1α functions as a selective endocytic adaptor for the internalization of synaptotagmin 1 at synapses
... DL-2-amino-5-phosphonovaleric acid; AZ: Active zone; BCA: Bicinchoninic acid; BSA: Bovine serum albumin; Ca 2+ : Calcium; CME: Clathrin-mediated endocytosis; CNQX: ...
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Immunological and molecular discrimination of Mycoplasma bovis from other mycoplasma species in cattle
... LIST OF ABBREVIATIONS 2YTA 2YT broth containing 150 ~g/mL ampicillin ABTS 2,2-Azino-di-[3-ethylbenzthizolin sulfonate 6] APS Ammonium persulphate BCA Bicinchoninic acid BMSCC Bulk milk s[r] ...
271
Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans
... cyclase; BCA: Bicinchoninic acid assay; BSF: Bloodstream form trypanosomes; cDNA: Complemetary deoxyribonucleic acid; CESP: Congolense ecpimastigote specific protein; CSP: Cell surface ...
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Influence of erythropoietin on microvesicles derived from mesenchymal stem cells protecting renal function of chronic kidney disease
... BCA: bicinchoninic acid; CKD: chronic kidney disease; DMEM/F12: Dulbecco ’ s modified Eagle ’ s medium nutrient mixture F-12; EPCs: endothelial progenitor cells; EPO: erythropoietin; EPO[r] ...
14
Coreopsis tinctoria Nutt ameliorates high glucose induced renal fibrosis and inflammation via the TGF β1/SMADS/AMPK/NF κB pathways
... protein; BCA: Bicinchoninic acid; CCK8: Cell counting kit; CHM: Chinese herbal medicine; DM: Diabetes mellitus; DMEM: Dulbecco ’ s Modified Eagle ’ s Medium; DN: Diabetic nephropathy; Dor: ...
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Effects of intermittent pressure imitating rolling manipulation on calcium ion homeostasis in human skeletal muscle cells
... Abbreviations: BCA, Bicinchoninic acid; Ca2+, Calcium ion; CIG, Control injured group; CK, Creatinkinase; CNG, Control normal group; CO2, Carbon dioxide; DMEM, Dulbecco’s minimum essenti[r] ...
9
Intravenous injection of microvesicle-delivery miR-130b alleviates high-fat diet-induced obesity in C57BL/6 mice through translational repression of PPAR-γ
... variance; BCA: Bicinchoninic acid; C/EBP- β : CCTTA enhancer binding protein- β ; DMEM/F-12: Dulbecco's modified eagle's medium: nutrient mixture F-12; FAS: Fatty acid synthase; FBS: Fetal ...
12
Building Positive Future Orientations: The Role of Natural Mentors in the Lives of African American Girls
... 1mM DTT and Complete, Mini, EDTA-free protease inhibitor cocktail (catalog number 11 836 170 001; Roche Diagnostics Corporation, Indianapolis, IN). The suspensions were centrifuged for approximately three seconds and ...
41
Alk5/Runx1 signaling mediated by extracellular vesicles promotes vascular repair in acute respiratory distress syndrome
... Specific antibodies (Abs) were as follows: Alk5 rabbit Ab (Abcam; Cambridge, MA); ITSN-1 (Bethyl Laborato- ries, Inc., Montgomery, TX); actin and Prestige ITSN-1 Abs (Sigma-Aldrich; St. Louis, MO); TGFβRII, Runx1, Ki67, ...
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An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cells
... Exosomes (the starting volume was kept constant for both UC and SUC method comparison) were lysed in RIPA buffer, 1 mM phenylmethylsulfonyl fluoride (PMSF), and protease inhibitors. The protein concentration of lysates ...
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Organotypic slice culture model demonstrates inter-neuronal spreading of alpha-synuclein aggregates
... When proper aggregation had been assured, the insol- uble WT or S129A α-syn was isolated by centrifugation in Eppendorf tubes. After discarding the supernatant, the pellet (containing the PFFs) was diluted to 2 mg/mL in ...
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Vascular endothelial growth factor expression and their action in the synovial membranes of patients with painful knee osteoarthritis
... or 10 ng/mL VEGF for 24 h. Using methodology described elsewhere [48], SMCs were lysed in radioimmunoprecipi- tation buffer (Thermo Fisher Scientific) containing a protease inhibitor cocktail (Sigma-Aldrich, St. Louis ...
8
Urine protein concentration estimation for biomarker discovery
... Bicinchoninic acid BCA, Lowy and Bradford and compared these to the current gold standard amino-... Urine samples with a diverse range of protein concentrations were collected from healt[r] ...
10
Further exploration of nontypable Haemophilus influenzae proteins P5 and P6 as vaccine candidates
... immunosorbent assay (ELISA), that 4G4 and 7F3 compete for binding to P6 and thus share a similar or overlapping epitope while 3B9 has an independent binding site. Mapping of the epitope for 3B9 showed that its binding is ...
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Transvenous n Butyl Cyanoacrylate Infusion for Complex Dural Carotid Cavernous Fistulas: Technical Considerations and Clinical Outcome
... occlusion of the CCF is not achieved, subsequent access through the same venous approach may be difficult. Thus, extra care needs to be taken when there is only one access to the CS, such as in a direct SOV puncture when ...
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