Behaviour and Immediate Early Gene Correlation
SRF activation in immediate early gene transcription
... Chapter 2: Results 2.3.2.2. Analysis of the SRF rep o rter activation by immunofluorescence To test whether reporter activity was uniform throughout the cell population, I used a single cell based technique employing ...
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Autoregulation of the human cytomegalovirus major immediate-early gene.
... HF COS Inf. Expression of pSVCC2 in COS-1 cells. COS-1 cells were transfected with pSVCC2 as described in Materials and Methods. HF) and cytoplasmic RNA (25 ,g) (TF COS) from transfected[r] ...
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An early gene maps within and is 3' coterminal with the immediate-early gene of equine herpesvirus 1.
... h postinfection; no metabolic inhibitors) conditions, in addition to the 6.0-kb IE mRNA, a 4.4-kb early (E) mRNA is transcribed from the IE gene region beginning at approximately 4 h pos[r] ...
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Structure and expression of murine cytomegalovirus immediate-early gene 2.
... Transac- tivation of a human cytomegalovirus early promoter by gene products from the immediate-early gene IE2 and augmentation by IEl: mutational analysis of the viral proteins. Inserti[r] ...
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Structural analysis of the major immediate early gene of human cytomegalovirus.
... It is hypothesized that this viral gene codes for the major regulatory protein controlling transcription of the viral genome at early times.. The properties of the viral gene and its pro[r] ...
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Repression of adenovirus early gene expression by coinfection with a temperature-sensitive mutant in the immediate-early gene of pseudorabies virus.
... Our ap- proach was to coinfect cells at the nonpermissive tempera- ture with wild-type adenovirus and a temperature-sensitive mutant in the immediate-early gene of either pseudorabies vi[r] ...
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The Human Cytomegalovirus Major Immediate-Early Distal Enhancer Region Is Required for Efficient Viral Replication and Immediate-Early Gene Expression
... MIE gene products, IE1 p72 and IE2 ...US3 gene expression during infection remains to be determined, but they are known or reputed to activate a wide variety of other viral and cellular genes (35, 47) and ...
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Sequence and structural organization of murine cytomegalovirus immediate-early gene 1.
... Also, after comparing maintain a high degree of sequence conservation in certain the protein sequences, only one region with homology regions of the polypeptide, including amino acids 67[r] ...
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trans-activation and autoregulation of gene expression by the immediate-early region 2 gene products of human cytomegalovirus.
... (a) Response of the HSV IE175-CAT and 38K-CAT hybrid genes (1.0 Fg of input target plasmid DNA) to cotransfection with 0.5 ,ug of effector plasmid DNA containing either the HCMV (Towne) [r] ...
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Characterization of the Transcriptional Repressive Element of the Human Cytomegalovirus Immediate-Early US3 Gene
... 3B; compare pBJ235 with pBJ294). As depicted in Fig. 3B, the tre influences transcription in a position-dependent but orien- tation-independent manner. FIG. 2. Transcriptional repression of the HIV-1 promoter by the US3 ...
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Regulation of a human cytomegalovirus immediate-early gene (US3) by a silencer-enhancer combination.
... downstream gene expression similarly to other reported silencers including those of c-mos, human ε-globin, SCG10, human triglyceride lipase, mouse renin, and collagen II (8, 9, 20, 34, 41, ...reporter gene ...
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Characterization of the regulatory functions of the equine herpesvirus 1 immediate-early gene product.
... To test whether the EHV-1 IE gene product can act as a trans-acting factor and to ascertain its effect on promoters representative of different temporal classes of EHV-1 genes, LM cells [r] ...
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Structure and regulation of the immediate-early frog virus 3 gene that encodes ICR489.
... Programmed in lane 1, immediate-early RNA (unhybridized); lane 2, RNA hybridized to the 1,640-base XbaI-PstI strand containing sequences complementary to ICR489; lane 3, RNA hybridized t[r] ...
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The Major Immediate-Early Gene ie3 of Mouse Cytomegalovirus Is Essential for Viral Growth
... pSM3fr-rev was not influenced by cotransfection of plasmid pBam25. A few plaques appeared after cotransfection of the ie3-deficient BAC plasmids and pBam25. Typically, the plaques were first seen at 7 to 8 days ...
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Variations in the cytomegalovirus major immediate early gene found by direct genomic sequencing
... An assay to detect and sequence DNA from human cytomegalovirus (HCMV) immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reactio[r] ...
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The 89,000-Mr murine cytomegalovirus immediate-early protein activates gene transcription.
... The 10.8-kb BamHI fragment (plasmid pAMB25) was used as a probe to detect MCMV sequences inserted into cellular DNA and mRNA transcribed in L cells. The other plasmids were used in trans[r] ...
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Regulated expression of early and late RNAs and proteins from the human cytomegalovirus immediate-early gene region.
... Expression of RNA and protein from the human cytomegalovirus immediate-early (IE) gene region (map units 0.732 to 0.751) was analyzed at early and late times after infection.. The level [r] ...
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The Human Cytomegalovirus Major Immediate-Early Enhancer Determines the Efficiency of Immediate-Early Gene Transcription and Viral Replication in Permissive Cells at Low Multiplicity of Infection
... on early viral gene ...on early viral gene expres- sion, we examined the recombinant viruses with the UL127 promoter driving the expression of ...
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Neurotrophin/Trk receptor signaling mediates C/EBPα, -β and NeuroD recruitment to immediate-early gene promoters in neuronal cells and requires C/EBPs to induce immediate-early gene transcription
... In summary, we have shown here that C/EBPα and Neu- roD form complexes in vivo and in vitro. These may define a novel class of C/EBP-bHLH transcription factor com- plexes of widespread importance, as we have recently ...
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Transactivation of a human cytomegalovirus early promoter by gene products from the immediate-early gene IE2 and augmentation by IE1: mutational analysis of the viral proteins.
... Since the wild-type IE2 protein of 54 kDa which contains the amino-terminal region failed to enhance expression from the E1.7 promoter, even when expressed at relatively high levels (Fig[r] ...
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