bicinchoninic acid
Effects of intermittent pressure imitating rolling manipulation on calcium ion homeostasis in human skeletal muscle cells
... Abbreviations: BCA, Bicinchoninic acid; Ca2+, Calcium ion; CIG, Control injured group; CK, Creatinkinase; CNG, Control normal group; CO2, Carbon dioxide; DMEM, Dulbecco’s minimum essenti[r] ...
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Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans
... BCA: Bicinchoninic acid assay; BSF: Bloodstream form trypanosomes; cDNA: Complemetary deoxyribonucleic acid; CESP: Congolense ecpimastigote specific protein; CSP: Cell surface protein; DAPI: ...
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Dendrobium nobile Lindley and its bibenzyl component moscatilin are able to protect retinal cells from ischemia/hypoxia by dowregulating placental growth factor and upregulating Norrie disease protein
... The level of PLGF was determined using ELISA [27]. One day after ischemia, the retina was separated from the enu- cleated eye cup, dissociated and then lysed by incubation in MPER (Hycell) for 30 min; the lysate was then ...
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Intravenous injection of microvesicle-delivery miR-130b alleviates high-fat diet-induced obesity in C57BL/6 mice through translational repression of PPAR-γ
... BCA: Bicinchoninic acid; C/EBP- β : CCTTA enhancer binding protein- β ; DMEM/F-12: Dulbecco's modified eagle's medium: nutrient mixture F-12; FAS: Fatty acid synthase; FBS: Fetal bovine serum; GAPDH: ...
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Immunological and molecular discrimination of Mycoplasma bovis from other mycoplasma species in cattle
... LIST OF ABBREVIATIONS 2YTA 2YT broth containing 150 ~g/mL ampicillin ABTS 2,2-Azino-di-[3-ethylbenzthizolin sulfonate 6] APS Ammonium persulphate BCA Bicinchoninic acid BMSCC Bulk milk s[r] ...
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Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta
... BCA: Bicinchoninic acid; bFGF: Basic fibroblast growth factor; BM: Bone marrow; cDNA: Complementary deoxyribonucleic acid; CSHL: Chorionic somatomammotropin hormone Like 1; CV: Chorionic villi; DAPI: ...
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Building Positive Future Orientations: The Role of Natural Mentors in the Lives of African American Girls
... 1mM DTT and Complete, Mini, EDTA-free protease inhibitor cocktail (catalog number 11 836 170 001; Roche Diagnostics Corporation, Indianapolis, IN). The suspensions were centrifuged for approximately three seconds and ...
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Influence of erythropoietin on microvesicles derived from mesenchymal stem cells protecting renal function of chronic kidney disease
... BCA: bicinchoninic acid; CKD: chronic kidney disease; DMEM/F12: Dulbecco ’ s modified Eagle ’ s medium nutrient mixture F-12; EPCs: endothelial progenitor cells; EPO: erythropoietin; EPO-MVs: microvesicles ...
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Human liver stem cells attenuate concanavalin A-induced acute liver injury by modulating myeloid-derived suppressor cells and CD4+ T cells in mice
... BCA: Bicinchoninic acid; CK: Cytokeratin; ConA: Concanavalin A; DMEM: Dulbecco ’ s modified Eagle medium; DMSO: Dimethyl sulfoxide; ECL: Enhanced chemiluminescence; ELISA: Enzyme-linked immunosorbent assay; ...
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ZiBu PiYin recipe prevents diabetes associated cognitive decline in rats: possible involvement of ameliorating mitochondrial dysfunction, insulin resistance pathway and histopathological changes
... The tissues of PFC and hippocampus were homogenized in ice cold lysis buffer [7]. Then, the homogenate was centrifuged at 15,000 g for 5 min at 4 °C prior to collect the supernatants. Protein concentrations in the ...
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Alk5/Runx1 signaling mediated by extracellular vesicles promotes vascular repair in acute respiratory distress syndrome
... Specific antibodies (Abs) were as follows: Alk5 rabbit Ab (Abcam; Cambridge, MA); ITSN-1 (Bethyl Laborato- ries, Inc., Montgomery, TX); actin and Prestige ITSN-1 Abs (Sigma-Aldrich; St. Louis, MO); TGFβRII, Runx1, Ki67, ...
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In silico discovery and validation of potent small molecule inhibitors targeting the activation function 2 site of human oestrogen receptor α
... Abbreviations AF2: Activation function-2; AR: Androgen receptor; BCa: Breast cancer; BCA: Bicinchoninic acid; bERα: Biotinylated oestrogen receptor-alpha; BF3: Binding function 3; BLI: B[r] ...
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Quantitative proteomics analysis of vitreous body from type 2 diabetic patients with proliferative diabetic retinopathy
... BCA: Bicinchoninic acid; C2: Complement C2; C4BPA: C4b-binding protein alpha chain; C4BPA: Complement component 4 binding protein alpha; CA1: Carbonic anhydrase 1; CAT: Catalase; CFD: Complement factor D; ...
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An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cells
... Exosomes (the starting volume was kept constant for both UC and SUC method comparison) were lysed in RIPA buffer, 1 mM phenylmethylsulfonyl fluoride (PMSF), and protease inhibitors. The protein concentration of lysates ...
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Coreopsis tinctoria Nutt ameliorates high glucose induced renal fibrosis and inflammation via the TGF β1/SMADS/AMPK/NF κB pathways
... Cells were collected and extracted with RIPA (Thermo, Rockford, IL, USA) lysis buffer containing a protease and phosphatase inhibitor cocktail. The protein concen- tration of the cell lysate was determined using the ...
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Vascular endothelial growth factor expression and their action in the synovial membranes of patients with painful knee osteoarthritis
... or 10 ng/mL VEGF for 24 h. Using methodology described elsewhere [48], SMCs were lysed in radioimmunoprecipi- tation buffer (Thermo Fisher Scientific) containing a protease inhibitor cocktail (Sigma-Aldrich, St. Louis ...
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SGIP1α functions as a selective endocytic adaptor for the internalization of synaptotagmin 1 at synapses
... DL-2-amino-5-phosphonovaleric acid; AZ: Active zone; BCA: Bicinchoninic acid; BSA: Bovine serum albumin; Ca 2+ : Calcium; CME: Clathrin-mediated endocytosis; CNQX: 6-cyano-7-nitroquinoxaline-2,3- ...
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Urine protein concentration estimation for biomarker discovery
... Bicinchoninic acid BCA, Lowy and Bradford and compared these to the current gold standard amino-... Urine samples with a diverse range of protein concentrations were collected from healt[r] ...
10
Ulcerative colitis: functional analysis of the in-depth proteome
... BCA: bicinchoninic acid; DHA: docosahexaenoic acid; ER: endoplasmic reticu- lum; ETC: electron transport chain; FA: formic acid; FABP: fatty acid binding protein; IBD: inflammatory ...
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A preliminary study of the solubilization of wool and separation of soluble wool into protein classes : this thesis was presented in part fulfilment of the requirements for the degree of Master of Science in Chemistry at Massey University
... 2-aminoethanol amino acid acetonitrile absorbance units bicinchoninic acid biuret bovine serum albumin P-mercaptoethanol bee venom carbonic anhydrase capillary electrophoresis capillary [r] ...
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