The binding of horseradish peroxidase -
Binding Interactions between Plant-Based Phenolic Chemicals and Horseradish Peroxidase
... the binding energy for this interaction is ...calculated binding energies are similar and between ...the binding energy. These results further suggest that the HIS42 binding distance does not ...
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The effect on structural and solvent water molecules of substrate binding to ferric horseradish peroxidase
... Alongside the frequency upshift, the marked narrowing of the ν NO lineshape suggests a less inhomogeneously-broadened environment with the substrate in place, 20 which is consistent with the replacement of water with ...
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A biochemical and immunological study of horseradish peroxidase
... Affinity chromatography matrixes with either an agarose derivative-bound aromatic hydroxamic acid or Sepharose-bound concanavalin A (Con A) have been developed to isolate HRP-isoenzymes. The ‘hydroxamate’ method exploits ...
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Cyclodextrin-responsive nanogel as an artificial chaperone for horseradish peroxidase
... 16. Nishikawa T, Akiyoshi K, Sunamoto J (1996) Macromolecular complexation between bovine serum albumin and self-assembled hydrogel nanoparticle of hydrophobized polysaccharides. J Am Chem Soc 118:6110–6115 17. Ikuta Y, ...
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Structure, Function and Applications of a Classic Enzyme: Horseradish Peroxidase
... Substrate Binding Sites HRP C can bind with the range of ...radish peroxidase C and other heme proteins like cytochrome P450 and globins where pyrrole nitrogen and heme iron atom undergo ...
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Horseradish Peroxidase Biosensor to Detect Zinc Ions in Aqueous Solutions
... KEYWORDS Horseradish Peroxidase; Maize Tassel; Multiwalled Carbon Nanotubes; Metal Biosensor; Zn 2+ ...by binding to the sulfhydryl groups, leading to inhibition in metabolism and the function of ...
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Analysis of the Partitioning Behavior of Horseradish Peroxidase to Phospholipid and Surfactant Membranes
... Possible binding mechanisms Two scenarios describing the partitioning behavior of proteins, including their insertion, are worthy of consideration: a free volume [12] or a crevice/pothole [17] ...HRP ...
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Electrochemical Immunoassay Determination of a Cancer Biomarker (CA19-9) by Horseradish Peroxidase
... nonspecific binding sites, this electrode went through incubation using CA19-9 solution at varied concentrations for 60 min, which was then dropped with the Fe 3 O 4 /MSN/HRP/Ab 2 ...
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Silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase for imaging and therapeutic applications
... fer indicates the high affinity or activity of the enzyme as compared to the HRP entrapped in silica NPs, which have a relatively higher K m value (16.91 mmol/mL). Since the enzyme is entrapped inside the silica matrix, ...
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A split horseradish peroxidase for the detection of intercellular protein protein interactions and sensitive visualization of synapses
... For each condition, HEK293T cells were plated into a single well of a 6-well plate, cultured, and transfected with the appropriate constructs using the procedure described above. Transfected cells were lysed, and the ...
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A Novel Tri-Protein Bio-Interphase Composed of Cytochrome c, Horseradish Peroxidase and Concanavalin A: Electron Transfer and Electrocatalytics
... In this study, a novel tri-protein bio-interphase was developed through layer-by-layer assembling HRP, Con A and Cyt c-HRP on gold (Au) electrode which was pre-modified with biocompatible 11-mercaptoundecanoic acid ...
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RSC Advances REVIEW. Horseradish peroxidase (HRP) as a tool in green chemistry. 1. Introduction. 2. HRP: structure and stability
... Besides the above mentioned nitration reactions, HRP can catalyse several asymmetric processes leading to the formation of valuable products as sulfoxides being many of them chiral. Kumar and co-workers claimed that ...
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Amperometric Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Entrapped in Titania Sol Gel Film on Screen Printed Electrode
... Their disposable feature also resolves the problem of pollution caused by previous tests. Based on the previous extensive studies, an essential principle of the develop- ment of SPE thick-film biosensors is enzyme ...
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Design and Construction of Magnetic Nanoparticles Incorporated with a Chitosan and Poly (vinyl) Alcohol Cryogel and its Application for Immobilization of Horseradish Peroxidase
... the horseradish peroxidase through covalent binding method (MNP-CS-PVA-HRP cryogel ...The horseradish peroxidase can catalytically oxidize the substrate of hydrogen peroxide and ...
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Polymerization of Phenol using Free and Immobilized Horseradish Peroxidase
... * E-mail of the corresponding author: [email protected] Abstract Phenol is a toxic compound and need to be treated before discharged. Phenol polymerization has been studied using free and immobilized Horse ...
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Electrochemical Behaviors of Horseradish Peroxidase on MoS2 Nanosheets Modified Electrode
... It is well-known that the electrochemical behaviors of redox protein are dependent on the solution pH, which was investigated on Nafion/HRP/MoS 2 /CILE by cyclic v[r] ...
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Use of horseradish peroxidase for gene-directed enzyme prodrug therapy with paracetamol
... Gene therapy is a potential method of treating cancer with a greater degree of targeting than conventional therapies. In addition, therapy can be directed towards cells within the tumour population that are traditionally ...
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5α-Reductase inhibitory compounds produced by polymerization of resveratrol with horseradish peroxidase
... Ethyl acetate extract of the oxidation products showed strong 5a-reductase inhibitory activity with 10%-15% organic solvents in the system, whereas without organic solv[r] ...
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DNA directed immobilization of horseradish peroxidase onto porous SiO2 optical transducers
... Abstract Multifunctional porous Si nanostructure is designed to optically monitor enzymatic activity of horseradish peroxidase. First, an oxidized PSi optical nanostructure, a Fabry-Pérot thin film, is ...
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Afferent Projections of the Clare-Bishop Area in the Cat Cortex: A Horseradish Peroxidase Study
... Results indicate that CB receives substantial input from the ipsilateral striate and parastriate visual cortex (areas 17, 18 and 19), the contralateral CB area, claustrum, thalamus (vent[r] ...
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