biosynthetic gene cluster
Advances in research of the structural gene characteristics of the aflatoxin biosynthetic gene cluster
... structural gene cloning, multiple methods are carried out, such as genetic complementation (aϔlB, aϔlD, aϔlI, ...by gene knockout, gene complementation studies, or enzymatic studies ...structural ...
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Analysis of the arginine biosynthetic gene cluster argCJBDFR of Corynebacterium crenatum
... showed a very high homology with the arginine biosyn- thetic gene cluster: argCJBDFR of C. glutamicum ATCC 13032 by blastn analysis in NCBI. This result indicated that the 6080 bp sequence of C. crenatum ...
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Heterologous Expression of Pseudouridimycin and Description of the Corresponding Minimal Biosynthetic Gene Cluster
... The biosynthetic gene cluster (BGC) was previously identified and characterized by knockout ...its biosynthetic precursors, several versions of a redesigned PUM BGC were generated and ...
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Identification of the decumbenone biosynthetic gene cluster in Penicillium decumbens and the importance for production of calbistrin
... Alder cycloaddition, catalyzed by the polyketide synthase (PKS) responsible formation of the nonaketide backbone of these molecules [17]. Motivated by the reported activities of calbistrin A, the interesting structural ...
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Organization and characterization of a biosynthetic gene cluster for bafilomycin from Streptomyces griseus DSM 2608
... bafilomycin biosynthetic gene cluster cloned here, the genes responsible for the biosynthesis of 2-amino-3- hydroxy-cyclopenta-2-enone were also ...
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Discovery of unusual biaryl polyketides by activation of a silent streptomyces venezuelae biosynthetic gene cluster
... venemycin biosynthetic gene cluster and vemR in the heterologous host Streptomyces coelicolor also re- sulted in venemycin ...the gene cluster encodes two halogenases and a flavin ...
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Heterologous expression of pikromycin biosynthetic gene cluster using Streptomyces artificial chromosome system
... large-sized gene cluster has emerged as a major point of ...target cluster in Strep- tomyces ...a biosynthetic gene cluster still requires a series of conjuga- tion and selection ...
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Structure and function of BorB, the type II thioesterase from the borrelidin biosynthetic gene cluster
... (4) De los Santos, E. L., and Challis, G. L. (2017) clusterTools: Proximity Searches For Functional Elements To Identify Putative Biosynthetic Gene Clusters. BioRxiv. (5) Medema, M. H., Kottmann, R., ...
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Identification of nocamycin biosynthetic gene cluster from Saccharothrix syringae NRRL B-16468 and generation of new nocamycin derivatives by manipulating gene cluster
... exact biosynthetic mechanism of ...the gene cluster responsible for nocamycin biosynthesis from ...its gene cluster. The biosynthetic gene cluster for nocamycin ...
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CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus
... 56-kb gene cluster ...whole gene cluster, including the promoter sequence, from ...expressed biosynthetic gene cluster for the production of ...big gene clus- ter ...
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Glutamic acid promotes monacolin K production and monacolin K biosynthetic gene cluster expression in Monascus
... Chan Zhang 1,2,3 , Jian Liang 1,2 , Le Yang 1,3 , Shiyuan Chai 1,3 , Chenxi Zhang 1,3 , Baoguo Sun 1,2,3 and Chengtao Wang 1,2,3,4* Abstract This study investigated the effects of glutamic acid on production of monacolin ...
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Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli
... encoding biosynthetic gene cluster. Biosynthetic gene clusters can be captured using several in vivo methods based on recombineer- ing, such as liner-linear homologous recombineering ...
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Characterization of the biosynthetic gene cluster of the polyene macrolide antibiotic reedsmycins from a marine-derived Streptomyces strain
... its biosynthetic machinery, herein, the rdm biosynthetic gene cluster was cloned and ...rdm cluster is located within a 104 kb DNA region harboring 21 open reading frames (ORFs), among ...
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Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into Streptomyces ansochromogenes lead to an improved nikkomycin production
... secondary biosynthetic gene cluster using Red/ET tech- nology into pSET152 or other vectors which contained the DNA fragments of oriT (RK2) region, the phage C31 attachment site and integrase ...
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Precise cloning and tandem integration of large polyketide biosynthetic gene cluster using Streptomyces artificial chromosome system
... metabolite biosynthetic gene cluster has become a useful strategy for production improvement and pathway modification of potentially valuable natural products present at minute quantities in original ...
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Novel polyoxins generated by heterologously expressing polyoxin biosynthetic gene cluster in the sanN inactivated mutant of Streptomyces ansochromogenes
... Therefore, we introduced the polyoxin biosynthetic gene cluster into the ΔsanN strain of S. ansochromogenes in this study. Here, we report that two new polyoxins (polyoxin O and P) were isolated from ...
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A standardized workflow for submitting data to the Minimum Information about a Biosynthetic Gene cluster (MIBiG) repository: prospects for research-based educational experiences
... by gene clusters and are found in a diverse set of ...a Biosynthetic Gene cluster repository facilitates standardized and centralized storage of experimental data on these gene clusters ...
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jmb Heterologous Expression of Daptomycin Biosynthetic Gene Cluster S Via Streptomyces Artificial Chromosome Vector System Research Article Review
... target cluster can be achieved through site-specific chromosomal integration of the vector and unique restriction sites as well as in vivo plasmid ...daptomycin biosynthetic gene cluster was ...
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Characterization of the aurantimycin biosynthetic gene cluster and enhancing its production by manipulating two pathway-specific activators in Streptomyces aurantiacus JA 4570
... its biosynthetic pathway and regulatory ...ATM biosynthetic gene cluster from ...target gene cluster (art) is responsible for ATM ...
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Cloning and analysis of the cypemycin biosynthetic gene cluster
... Indeed, Gram-positive bacteria are known to use diverse types of modified peptides specifically for quorum sensing. This does not exclude a supplementary function, since some lantibiotics have been shown to serve as ...
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