biotin-streptavidin
Rapid detection of herpes simplex virus in clinical specimens by use of a capture biotin streptavidin enzyme linked immunosorbent assay
... The presence of HSV antigen in clinical specimens and stock virus preparations was confirmed by blocking the detection with rabbit and human HSV-1- and -2-specific antisera.. Preimmune s[r] ...
6
Quantitative determination of staphylococcal enterotoxin A by an enzyme linked immunosorbent assay using a combination of polyclonal and monoclonal antibodies and biotin streptavidin interaction
... The fourfold increase in sensitivity of the assay in which indirectly biotinylated rabbit anti-SEA antibody was used as opposed to directly biotinylated rabbit anti-SEA 0.25 versus 1 ng/[r] ...
6
Development of a biotin streptavidin enhanced enzyme linked immunosorbent assay which uses monoclonal antibodies for detection of group C rotaviruses
... The other rotavirus serogroups used in this study included group A rotaviruses passaged in gnotobiotic pigs porcine OSU and Gottfried and human Wa strains, porcine group B rotavirus Ohio[r] ...
7
Typing of herpes simplex virus by capture biotin streptavidin enzyme linked immunosorbent assay and comparison with restriction endonuclease analysis and immunofluorescence method using monoclonal antibodies
... In this report, we describe the typing of clinical isolates by a capture enzyme-linked immunosorbent assay using biotin and streptavidin capture B/SA ELISA with both polyclonal and monoc[r] ...
5
Rolling Circle Amplification on Biotin Streptavidin Complexes Immobilized to Activated Cyclic Polyolefin Surfaces
... croarrays [12]. A commonly used COP surface treatment is UV-irradiation grafting of a benzophenone (BP)/acryl amide or methylacrylate [3,6,8,16,17]. Nexus Biosystems (San Diego CA) sells COP microtiter plates (Aurora Mi- ...
11
Identification of Koi Herpesvirus on Koi Fish (Cyprinus carpio) with Immunocytochemistry Test Streptavidin Biotin
... In this research, we used 20 koi fishes (C. carpio) with the size are about 10-20 cm and showedsome symptoms clinical infected with KHV, they are gill slime, erosion or ulcers on the skin, movement were not controlled, ...
7
Streptavidin biotin based directional double Nanobody sandwich ELISA for clinical rapid and sensitive detection of influenza H5N1
... of biotin to streptavidin is one of the strongest non-covalent interactions known in nature ...of biotin-streptavidin can resist some severe environments such as organic solvents, pro- ...
10
Liposomes functionalized to overcome the blood–brain barrier and to target amyloid-β peptide: the chemical design affects the permeability across an in vitro model
... by biotin/streptavidin ligation (Figure 1A) or by covalent coupling (Figure 1B); for both techniques, the RI7217 binding occurred at the PEG terminus of PE-PEG lipid inserted in ...
10
Construction of an HRP-streptavidin bound antigen and its application in an ELISA for porcine circovirus 2 antibodies
... Like biotin, streptavidin binding peptide (SBP) has a binding affinity to streptavidin (SA), and this prop- erty was exploited to fuse it to the antigen ...HRP-labeled streptavidin (HRP-SA) ...
7
Biological Chemistry A Metal-free Click Chemistry Approach for the Assembly and Probing of Biomolecules
... a biotin at the opposite end to immobilize FNA and for probing experiments, ...with streptavidin via a PEG linker to enable the formation of biotin- streptavidin during the approach ...
7
Quantification of specific bindings of biomolecules by magnetorelaxometry
... biomolecules streptavidin and anti-biotin antibody, both labelled by magnetic nanoparticles (MNP), to biotin coated on agarose beads, was quantified by magnetorelaxometry ...the ...
12
Designer nanoparticle: nanobiotechnology tool for cell biology
... Late endosomes Gradient centrifugation SPMNP isolation assay; Biotin-streptavidin; antibody-SPMNPs; Negatively charged SPMNPs Multi-vesicular bodies Gradient centrifugation SPMNP isolati[r] ...
12
Use of streptavidin bound to biotinylated DNA structures as model substrates for analysis of nucleoprotein complex disruption by helicases
... displacing streptavidin from biotin. Use of forked duplex DNA-biotin-streptavidin substrates to test for such cooperativity could potentially display both cooperative DNA unwinding and ...
22
Intracellular Glycosylphosphatidylinositols Accumulate on Endosomes: Toxicity of Alpha-Toxin to Leishmania major
... alpha-toxin-biotin. Streptavidin-conjugated Alexa Fluor 594 (red) and Alexa Fluor 488-conjugated goat anti-rabbit secondary antibodies (green) were ...with streptavidin-conjugated Alexa Fluor 594 ...
11
Evaluation of the Effect of Biotin in Dyslipidemia
... of biotin on pyruvate carboxylase, acetyl-CoA carboxylase, propionylCoA carboxylase, and markers for glucose and lipid homeostasis in type 2 diabetic patients and nondiabetic ...
129
Streptavidin Modified ZnO Film Bulk Acoustic Resonator for Detection of Tumor Marker Mucin 1
... the biotin, along with the dually labeled chelates, is captured by the streptavindin modi- fied ...the streptavidin modified FBAR when the target MUC1 is ...on streptavidin modified FBAR when the ...
8
Polyester synthases and polyester granule assembly : a thesis presented to Massey University in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Microbiology
... length streptavidin is characterised by a poor solubility and strong tendency to form higher-order aggregates (Argarana et ...length streptavidin forms inactive inclusion bodies when produced in ...length ...
148
Production of Genetically Engineered Biotinylated Interleukin-2 and Its Application in a Rapid Nonradioactive Assay for T-Cell Activation
... for the production and purification of fusion proteins, which uniformly contain a single biotin. In chemical biotinylation, the number of biotins per protein molecule is variable. Also, in the Promega PinPoint ...
6
A Multifunctional Single-Attachment-Point Reagent for Controlled Protein Biotinylation
... of biotin and fluorescein, which can be used to determine the degree of biotinylation of proteins based on fluorescein’s absorbance or ...fluorescein, biotin and the NHS ester are attached to a peptide ...
13
Genetic dissection of attractant induced conductances in Paramecium
... in biotin show a very different pattern of conductances ...of biotin (about 5–10·pA) and a larger, transient inward conductance of approximately 10·pA upon removal of ...–1 biotin, a tenfold lower ...
9