blastocyst stage
Assessment of Epidermal Growth Factor (EGF) Effects on Development of Vitrified Mouse Morulae to the Blastocyst Stage
... Our results indicate that EGF can enhance development of mouse embryo to blastocyst stage in a dose-dependent manner. These results are in agreement with the other investigators [12-14]. Kim et al. [14] ...
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Culture of human preimplantation embryos to the blastocyst stage: a comparison of 3 media
... HTF was one of the first medium specifi- cally designed for human embryo culture (Quinn et al., 1985). Until recently, its use had not been fully evaluated, particularly for culturing those embryos which remained af- ter ...
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Test tube embryos mouse and human development in vitro to blastocyst stage and beyond
... cell stage human embryos were split in half and cultured in ...after blastocyst stage can be faithfully recapitulated without maternal input, if sufficient culture conditions are ...provided. ...
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Non-inferiority of cleavage-stage versus blastocyst-stage embryo transfer in poor prognosis IVF patients (PRECiSE trial): study protocol for a randomized controlled trial
... a blastocyst [9] ...for blastocyst trans- fers than cleavage-stage transfers (OR ...cleavage stage embryos might benefit poorer prognosis patients, it also may be associated with a range of ...
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A role for PDGF signaling in expansion of the extra embryonic endoderm lineage of the mouse blastocyst
... mammalian blastocyst comprises two lineages: the pluripotent epiblast (EPI) and primitive endoderm ...mouse blastocyst stage embryos and represent the PrE ...
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Effects of essential and non-essential amino acids on in-vitro maturation, fertilization and development of immature bovine oocytes
... producing blastocyst with higher viability (33, ...to blastocyst stage of mouse embryos ...the blastocyst, development of the early cleavage stages was stimulated by the non- essential amino ...
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The effect of superovulation on the contributions of individual blastomeres from 2 cell stage CF1 mouse embryos to the blastocyst
... 2-cell stage contributed predominantly to either the abembryonic or embryonic pole approximately one- third of the time in both CF1 and NIH Swiss ...2-cell stage tending to contribute cells predominantly to ...
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Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system
... cells stage injected pig-mouse chimeric blastocysts; E3.5-CBs: Blastocyst stage injected pig-mouse chimeric blastocysts; Endo genes: Endogenous genes; EPS: Expanded pluripotent stem cells; ES: ...
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Is it time for a paradigm shift in understanding embryo selection?
... extended blastocyst stage embryo culture, and especially with increasing popularity of elective single embryo transfer (eSET), the concept of embryo selection has increasingly become a mainstay of routine ...
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Fast Cryopreservation of the Mammalian Embryos in Different Developmental Stages by 0.25 mL Straws Vitrification with One Equilibration Step
... morula stage that had a hatching rate of ...and blastocyst stage hatched after thawing and in vitro culture, regardless of the vitrification media ...hatching stage, regardless of the ...
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Effect of Oocyte Recovery Techniques on in Vitro Production of Swine Embryos
... the blastocyst stage after IVF when compared to the ovary slicing method (p = ...on blastocyst cell number was ...the blastocyst stage after IVF by the slicing method was lower when ...
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New insights into mechanisms that regulate DNA methylation patterning
... the blastocyst stage where cells in the ICM [inner cell mass, from which embryonic stem (ES) cells are isolated in vitro] are separated functionally from the differentiated trophectoderm cells, which will ...
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Formation of the embryonic abembryonic axis of the mouse blastocyst: relationships between orientation of early cleavage divisions and pattern of symmetric/asymmetric divisions
... In early mouse development, pluripotent cells become set apart in the inside compartment of the embryo. This happens because some cells divide asymmetrically rather than symmetrically in the fourth and fifth rounds of ...
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The effects of dimethyl sulfoxide and ethylene glycol as vitrification protectants on different cleavage stages of mouse embryo quality
... the blastocyst stage (Table ...any stage shrink rapidly and considerably, and are remained shrunken, because VS solutions contain not only a permeating agent ...
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Assisted Reproductive Technologies Disrupt Genomic Imprinting in Human and Mitochondria in Mouse Embryos
... the blastocyst stage, there was no apparent difference in CHDH levels or the repressive histone 3 lysine 9 dimethylation (H3K9me2) mark in 10 IU blastocysts compared to ...cleavage stage embryos, in ...
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Reproductive outcomes in women with unicornuate uterus undergoing in vitro fertilization: a nested case-control retrospective study
... cleavage stage embryos had significantly higher miscarriage rate and lower live-birth rate compared to those who had frozen-thaw cy- cles, which held true for women with either unicornuate or normal ...of ...
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The pluripotent state in mouse and human
... Nonetheless, much can be learned from the analysis of the results to date. Huang et al. performed a meta-analysis in which they compared gene expression patterns in mouse and human naïve and primed cells, and ...
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IVF/ICSI outcomes after culture of human embryos at low oxygen tension: a meta-analysis
... Meta-analysis also presents problems, such as the qual- ity of the primary studies or the form in which data are reported and the dependence on a sufficient number of eligible studies to justify the statistical analysis. ...
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Molecular cloning and expression of bovine nucleoplasmin 2 (NPM2): a maternal effect gene regulated by miR-181a
... MII stage oocytes, pronuclear, 2-cell, 4-cell, 8-cell, 16-cell, morula and blastocyst stage embryos (n = 5 pools of 10 each) were obtained by in vitro fertilization of abattoir derived oocytes as ...
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Preimplantation genetic diagnosis and the biopsy technique: Important considerations
... cleavage stage has been the mostly performed ...(blastocyst stage biopsy), or representing a valid alternative to overcome ethical issues (polar body biopsy) have obtained increasing ...
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