Brefeldin A
Brefeldin A Inhibits Cell-Free, De Novo Synthesis of Poliovirus
... Paradoxically, there appears to be a requirement for some aspects of secretory function in the replication of poliovirus. This has been inferred by the inhibition of poliovirus replica- tion by the fungal metabolite ...
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Brefeldin a decreases the activity of the general amino acid permease (GAP1) and the more specific systems for L-leucine uptake in Saccharomyces cerevisiae
... 14. Pannunzio, V.G., Burgos, H.I., Alonso, M., Mattoon, J.R., Ramos, E.H. and Stella, C.A. A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an ...
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Caveolar Endocytosis of Simian Virus 40 Is Followed by Brefeldin A-Sensitive Transport to the Endoplasmic Reticulum, Where the Virus Disassembles
... In general, there are few ligands known that are taken up by non-clathrin vesicle endocytosis, and there are few examples of ligands that traffic to the ER. Included among these are certain bacterial toxins such as ...
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Differential Modulation of Surface and Intracellular Protein Expression by T Cells after Stimulation in the Presence of Monensin or Brefeldin A
... Intracellular cytokine staining is an increasingly popular analytical tool that can be used to define the profile of cytokines in various disease states. One important requirement for this assay is the inclusion of a ...
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Identification of novel antiviral of fungus-derived brefeldin A against dengue viruses
... identified brefeldin A as a novel antiviral agent against dengue ...of brefeldin A was confirmed by virus titer and immunofluorescence ...assay. Brefeldin A inhibited dengue viruses regardless of ...
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Growth inhibition of androgen-responsive prostate cancer cells with brefeldin A targeting cell cycle and androgen receptor
... Brefeldin A (BFA) [8], a fungal antibiotic, has been initially known to play a regulatory role in the intracel- lular transport system [9-11]. It induces the reversible disassembly of the Golgi complex, resulting ...
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Temporal Morphogenesis of Herpes Simplex Virus Type 1-Infected and Brefeldin A-Treated Human Fibroblasts
... effort to determine the envelopment and transport of HSV-1 virions together with the viral glycoproteins and virus–cell interactions. Unfortunately (16,22), an ultrastructural time sequence study is clear cut only during ...
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Inhibition of poliovirus RNA synthesis by brefeldin A.
... During poliovirus infection in the absence of the RNA replication inhibitor, both the accumulation of labeled poliovirus proteins and the inhibition of host cell protein synthesis 17, 39[r] ...
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Brefeldin A arrests the maturation and egress of herpes simplex virus particles during infection.
... Other cell lines defective in a variety of enzymes required for processing of N-linked oligosaccharides have been shown to produce less infectious virus than normal when infected with HS[r] ...
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Entry of cholera toxin into polarized human intestinal epithelial cells Identification of an early brefeldin A sensitive event required for A1 peptide generation
... Sequential events are required for signal transduction from the apical membrane: a binding to cell surfaces and rapid endocytosis; b early, BFA-sensitive vesicular transport essential fo[r] ...
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Human Papillomavirus Type 16 Infection of Human Keratinocytes Requires Clathrin and Caveolin-1 and Is Brefeldin A Sensitive
... Having observed the colocalization of HPV16 reporter virions with an early endosome marker and a caveolar marker, we wondered if virus entry into HaCaT cells was performed by both clathr[r] ...
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BFR1, a multicopy suppressor of brefeldin A-induced lethality, is implicated in secretion and nuclear segregation in Saccharomyces cerevisiae.
... Our results and those of others (GRAHAM et al. 1993) indicate that BFA inhibits ER- to-Golgi and intra-Golgi transport in S. cerevisiae as it does in mammalian cells. The secre[r] ...
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Effect of brefeldin A on alphaherpesvirus membrane protein glycosylation and virus egress.
... Monensin inhibits the processing of herpes simplex virus glycoproteins, their transport to the cell surface, and the egress of virions from infected cells.. Role of cytoplasmic vacuoles [r] ...
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Assembly and polarized release of Punta Toro virus and effects of brefeldin A.
... Our results clearly show that the distribution of PTV Gl and G2 proteins, whether during PTV infection or when expressed from a vaccinia virus recombinant, gradually changes from a more [r] ...
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Imprint of cytomegalovirus and varicella zoster virus infections on the immune system of healthy and diseased subjects
... Referring to chapter 5, the aim was to determine differences in the magnitude of T cell responses to CMV in patients with coronary artery disease and age-matched healthy volunteers. A BD LSR II flow cytometer was ...
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Cyclic AMP increases COX 2 expression via mitogen activated kinase in human myometrial cells
... We confirmed that forskolin activated PKA and AMPK by using phospho-CREB and phospho-ACC (Fig. S1A and B). We used phospho-AKT as marker of EPAC activation, but found no increase of phosphorylation (Fig. S1C); to confirm ...
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Sub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice
... 5 Effects of salt stress on the dynamics of brefeldin A (BFA) compartment labelling by Os PIP2;5 constructs in rice root cells.. Root systems of.[r] ...
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The novel BTB-kelch protein, KBTBD8, is located in the Golgi apparatus and translocates to the spindle apparatus during mitosis
... with Brefeldin A (BFA) has the same effect: it blocks the protein transport from the ER to the Golgi [22] and therefore causes a collapse of the Golgi stacks resulting in punctuated ...
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Viperin Targets Flavivirus Virulence by Inducing Assembly of Noninfectious Capsid Particles
... ABSTRACT Efficient antiviral immunity requires interference with virus replication at multiple layers targeting diverse steps in the viral life cycle. We describe here a novel flavivirus inhibition mechanism that results ...
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Mineral Fabrication and Golgi Apparatus Activity in the Mouse Calvarium
... by brefeldin A (an estab- lished golgi inhibitor) and restored by forskolin, enabling an association with mineral fabrication to be quantified as changing fluorescence intensity (AU) of GFP or TC ...
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