BT-474
Total Phenolics and Flavonoids Contents of Aqueous Extract of Stinging nettle and In Vitro Antiproliferative Effect on Hela and BT- 474 cell lines
... Previous reports indicated a direct association between the amount of polyphenols present in fruits and vegetables and their cancer prevention ability (11-13). In this study we investigated the amount of total phenolics ...
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Extracellular matrix protein 1 regulates cell proliferation and trastuzumab resistance through activation of epidermal growth factor signaling
... Human breast carcinoma cell lines BT-474, MCF-7, SKBR3, MDA-MB-231, T47D, MDA-MB-468 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). All cells were cultured according to ...
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Liposomes derivatized with multimeric copies of KCCYSL peptide as targeting agents for HER-2-overexpressing tumor cells
... and BT-474 cells was preliminarily tested by flow cytometry using an anti- Herceptin FITC-conjugated antibody and its isotype control (IgG1-FITC ...conjugated). BT-474 cells were characterized ...
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Study of the Effect of Silymarin on Viability of Breast Cancer Cell Lines
... and BT-474—were incubated for 24 hours in standard conditions be- fore adding 100, 200, 400, 800, 1600 µM Silymarin to each ...and BT-474 growth at different concentrations after 24, 48 and 72 ...
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miR-1296-5p decreases ERBB2 expression to inhibit the cell proliferation in ERBB2-positive breast cancer
... In this study, we find that miR-1296-5p expression is suppressed in a majority of breast cancer samples when compared to normal breast samples. Previous study has showed that the expression level of miR-1296 is Fig. 1 ...
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A combination of p53-activating APR-246 and phosphatidylserine-targeting antibody potently inhibits tumor development in hormone-dependent mutant p53-expressing breast cancer xenografts
... plates were coated with an oligonucleotide that contained a p53 consensus DNA-binding site. Nuclear extracts were prepared from BT-474 cells using an extraction kit (Active Motif), and 2.5 µg was incubated ...
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Stat3 and CCAAT/enhancer binding protein beta (C/EBP beta) regulate Jab1/CSN5 expression in mammary carcinoma cells
... including BT-474, ZR-75-1, MCF7, BT- 549, and MDA-MB-453 revealed higher Jab1 promoter activity compared to normal mammary epithelial cells MCF-10A, HMEC and 184A (Figure ...
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Wogonoside inhibits invasion and migration through suppressing TRAF2/4 expression in breast cancer
... Tumor metastasis accounts for 90% of cancer-associated deaths, in which invasion plays a critical role in metastasis [27]. During invasion, tumor cells firstly lose cell-cell junc- tions, subsequently degrade, remodel, ...
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Estrogen regulates Hippo signaling via GPER in breast cancer
... Bcap-37, BT-474, ZR-75-1, and MDA-MB-361 breast cancer cells and HeLa cervical cancer cells (the expression of ERα, ERβ, and GPER in these cell lines is shown in Supplemental Figure 2A) with G1 and observed ...
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mTOR complex-2 stimulates acetyl-CoA and de novo lipogenesis through ATP citrate lyase in HER2/PIK3CA-hyperactive breast cancer
... T47D, BT-474, BT-20, ZR-75- 1) exhibited elevated and constitutive levels of P-ACL, while 3 cell lines (MDA231, HCC1806, MDA435) expressed low P-ACL levels and 1 line (MCF7) showed medium level of ...
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Novel cross-talk between IGF-IR and DDR1 regulates IGF-IR trafficking, signaling and biological responses
... MCF-7, BT-474 and MDA-MB-231 breast cancer cells were transiently transfected as in c Cell migration in response to 10 nM of IGF-I was evaluated as in b Values are mean±SEM of three inde[r] ...
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Combining RANK/RANKL and ERBB 2 targeting as a novel strategy in ERBB 2 positive breast carcinomas
... Next, we performed in vitro functional analyses in order to assess if the observed downregulation of NF-κB sig- naling (Fig. 4) following denosumab and trastuzumab treatment had an impact in BC cell properties. Prior to ...
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α-linolenic acid and docosahexaenoic acid, alone and combined with trastuzumab, reduce HER2-overexpressing breast cancer cell growth but differentially regulate HER2 signaling pathways
... We acknowledge several limitations of the current study. One limitation is that only one ALA metabolite was studied. EPA is another n-3 ALA metabolite but we focused specifically on DHA for a number of reasons. EPA is a ...
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The combined immunodetection of AP 2α and YY1 transcription factors is associated with ERBB2 gene overexpression in primary breast tumors
... siRNAs. BT-474 breast cancer cells overexpressing ERBB2, both through amplification and enhanced transcrip- tion, were transfected with AP-2α and AP-2γ siRNAs, both independently and in combination for ...
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Ku proteins interact with activator protein 2 transcription factors and contribute to ERBB2overexpression in breast cancer cell lines
... To complete this investigation, the reporter vectors activities were measured in BT-474 (Figure 5) and SKBR3 [see Addi- tional data file 4] cells 72 hours after transfection of Ku and AP-2 siRNAs (Figure ...
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Cleavage of the extracellular domain of junctional adhesion molecule A is associated with resistance to anti HER2 therapies in breast cancer settings
... for BT-474 – sensitive and ...trastuzumab-sensitive BT-474 (A) and lapatinib-sensitive SK-BR-3 cells (B) to recombinant soluble JAM-A ...
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CDH1 and IL1-beta expression dictates FAK and MAPKK-dependent cross-talk between cancer cells and human mesenchymal stem cells
... MCF7, BT-474, T-47D, and SKB-R3, followed by HT-29 and BT-20 ...(MCF7, BT-20, HT-29, and BT-474) and negative cell lines that did not induce morphological changes (FaDu, ...
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<p>EFEMP2 Inhibits Breast Cancer Invasion And Metastasis In Vitro And In Vivo</p>
... and BT-474 breast cancer cells transfected with EFEMP2 cDNA and control vector, EFEMP2 upregulation decreased the level of GSK3β phosphorylation and then promoted the degradation of β-catenin, so as to ...
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Monitoring Src status after dasatinib treatment in HER2+ breast cancer with 89Zr trastuzumab PET imaging
... All animal handling and manipulations were con- ducted in accordance with the guidelines set by Wayne State University Institutional Animal Use and Care Committee. For imaging experiments, female athymic nu/nu mice (6–8 ...
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Identification of a novel AMPK PEA15 axis in the anoikis resistant growth of mammary cells
... Further, MDAMB231 and BT 474 cells stably expressing the nonphosphorylatable mutant of PEA15 were also markedly impaired in their tumor-forming potential compared to WT PEA15-expressing [r] ...
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