Buffy Coat
Conversion from Platelet-Rich Plasma Platelet Production to Buffy Coat Platelet Component Production: Benefits and Limitations
... laboratory, buffy coat platelets production method may require more overall effort than PRP method, but the finished product is generally leukoreduced with better ...
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Buffy coat specimens remain viable as a DNA source for highly multiplexed genome wide genetic tests after long term storage
... frozen buffy coat specimens were randomly selected by the ACCORD Coordinating Center out of 6,008 participants who had consented to the broadest categories of genetics study usage of their ...
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A comparative study of blood smear, quantitative buffy coat and antigen detection for diagnosis of malaria
... The present study was conducted in the Department of Microbiology, at Bangalore Medical College and Research Institute, Bangalore over a period of one year between August 2012 and August 2013. A total number of 150 ...
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Evaluation of Diagnostic Tests in Malaria (Evaluating the Diagnostic Value of Quantitative Buffy Coat and Rapid Diagnostic Test using Plasmodium Lactate Dehydrogenase against the Peripheral Smear Microscopy
... This is to certify that the dissertation titled, “EVALUATION OF DIAGNOSTIC TESTS IN MALARIA (EVALUATING THE DIAGNOSTIC VALUE OF QUANTITATIVE BUFFY COAT AND RAPID DIAGNOSTIC TEST USING PLASMODIUM LACTATE ...
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Quantitative Buffy Coat: A Useful Tool for Diagnosis of Malaria
... 32 acridine orange and containing a float was developed for the rapid quantification of leucocytes in peripheral blood. This method was termed "QBC" for "Quantitative Buffy Coat Analysis" ...
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Malaria Diagnosis by Quantitative Buffy Coat Technique
... Malaria Diagnosis by Quantitative Buffy Coat Technique ORIGINAL ARTICLE Malaria Diagnosis by Quantitative Buffy Coat Technique A Alias, MSc*, S A B Afifi, MSPH*, R Zuraidah, DTMP*, A Shukri, DTMP*, A[.] ...
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In vitro viability effects on apheresis and buffy-coat derived platelets administered through infusion pumps
... Buffy coat platelets were collected according to criteria simi- lar to those for apheresis donation. In total, 450 mL of whole blood was drawn into either a CPD/SAG-M quadruple-bag blood container system ...
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Buffy Coat Transfusions in Neonates With Sepsis and Neutrophil Storage Pool Depletion
... The small number of patients at any one center available for randomized study is due to, in part, the low incidence of persistently neutropenic neonates with sepsis whose marrows are dep[r] ...
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Buffy Coat Transfusions in Neutropenic Neonates With Presumed Sepsis: A Prospective, Randomized Trial
... I:T ratio 0.80 were also not significantly different with respect to short- or long-term survival, the percentage of neutrophil storage pool in the bone marrow aspirate, or the percentag[r] ...
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Accuracy of a Polymerase Chain Reaction-based Assay for Detection of Pneumococcal Bacteremia in Children
... When their assay was tested on 16 clinical specimens from patients with culture proven pneumococcal bacteremia, the assay showed moderate sensitivity (75%) when applied to the buffy coat[r] ...
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Isolation of a Novel Orientia Species (O chuto sp nov ) from a Patient Infected in Dubai
... preantibiotic buffy coat sample as that used for the qPCR assay was examined after 2 weeks of growth using direct IF and revealed the presence of a scrub typhus group rickettsiae grow- ing within the ...
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Defective cellular immunity in renal failure: depression of reactivity of lymphocytes to phytohemagglutinin by renal failure serum
... Normal buffy coat cells were resuspended in sera obtained from normal subjects and from 14 patients with renal failure, then stimulated with phytohemagglutinin M and the cellular respons[r] ...
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Diagnosis of Hepatozoon canis in young dogs by cytology and PCR
... of buffy coat and blood smears is routinely used for the diagnosis of canine hepatozoono- ...or buffy coat should be preferred to cytology for the diagnosis of ...
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Serological and molecular survey of Leishmania parasites in apparently healthy dogs in the West Bank, Palestine
... the buffy coat is the third most convenient sam- ple after spleen and bone marrow, but preferred in this study due to being less invasive, readily repeatable, more accepted by dog ...
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Detection of Trypanosoma brucei spp in human blood by a nonradioactive branched DNA based technique
... We have developed a nonradioactive branched DNA (bDNA)-based assay for the diagnosis of the African trypanosomiases in simple buffy coat preparations of human blood. Two repetitive DNA sequences specific to ...
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Demodex Folliculorum in Cutaneous Biopsy: A Histological Surprise
... Methods: In 6 months study, blood sample were collected from 100 patients attending the OPD Laboratory in SMS Hospital, who were having malaria. Complete blood count and peripheral blood film examination (Thin and thick ...
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Comparison of YKL 39 and CHIT 1 expression during macrophages differentiation and polarization
... The chitinase-like proteins YKL-39 (chitinase 3- like-2) and Chitortriosidase (CHIT-1) are mem- bers of the chitinases family. YKL-39 expression has been associated with osteoarthritis, whereas CHIT-1 activity is ...
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Evaluation of Different PCR Assays for Early Detection of Acute and Relapsing Brucellosis in Humans in Comparison with Conventional Methods
... Again, buffy coat and whole blood were the optimal clinical samples, whereas serum does not appear to be a useful clinical sample for a posttreat- ment ...
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Early detection of active cytomegalovirus (CMV) infection after heart and kidney transplantation by testing for immediate early antigenemia and influence of cellular immunity on the occurrence of CMV infection
... In this study we evaluated the antigenemia test, together with quick cultures of urine, saliva, and buffy-coat cells, in the detection of active CMV infection in heart and kidney transpl[r] ...
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Identification and Characterization of Immunoglobulin G in Blood as a Major Inhibitor of Diagnostic PCR
... A major inhibitor of diagnostic PCR in human plasma was identified and the mechanism of inhibition was characterized. Human blood was divided by centrifugation into buffy coat, plasma, platelets, and ...
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