C acetobutylicum
Stochastic modelling of the growth of C. Acetobutylicum with missing data
... Stochastic influences play an important role in various areas especially in the area of biological process. Stochastic differential equation is the differential equation in which the terms of their characteristic ...
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Stochastic modeling of the C. Acetobutylicum and solvent productions in fermentation
... concentration, c is the growth associated coefficient for butanol formation (g/g cell) and d is the non-growth associated coefficient for butanol formation (g/g ...
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Coenzyme A transferase independent butyrate re assimilation in Clostridium acetobutylicum evidence from a mathematical model
... To further elucidate the role of the CoA-transferase in the re-assimilation of acids during the pH-induced metabolic switch, we used a recently developed standard operation procedure (SOP) for the anaerobic growth of ...
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Comparative shotgun proteomic analysis of Clostridium acetobutylicum from butanol fermentation using glucose and xylose
... on C. acetobutylicum DSM 1731 strain identified about 564 proteins that accounts for ...of C. acetobutylicum in chemostat culture identified 383 proteins ...of C. acetobutylicum ...
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Cap0037, a Novel Global Regulator of Clostridium acetobutylicum Metabolism
... Noticeably, Cap0037 is homologous to the YgaS-like protein in Bacillus cereus G9241 (a putative DNA-binding protein), although the relationship is distant (see Fig. S1B in the supplemental mate- rial). A helix-turn-helix ...
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Arabinose-Induced Catabolite Repression as a Mechanism for Pentose Hierarchy Control in Clostridium acetobutylicum ATCC 824
... in C. acetobutylicum, this could contribute to the preference for arabinose by creating a bottleneck for xylose import and requires further ...for C. acetobutylicum to target the ...
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Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824
... as the pyrE mutation is always repaired to prototrophy, using the ACE correction vector pMTL-ME6, with res- toration of wild-type solvent yields (Additional file 4: Fig. S5). More importantly, mutants created in pyrE ...
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Diauxic growth of Clostridium acetobutylicum ATCC 824 when grown on mixtures of glucose and cellobiose
... to the suggestion that they may also play a role in car- bon catabolite repression (Behrens et al. 2001; Saier and Reizer 1992; Singh et al. 2008; Tangney et al. 2003).This hypothesis was reinforced by the fact that a ...
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Quantitative proteomic analysis of the influence of lignin on biofuel production by Clostridium acetobutylicum ATCC 824
... It has been demonstrated that the relative concentra- tions of nucleotides (particularly ATP and GTP) play important roles in cell physiology and regulation [63]. In C. acetobutylicum, glycolysis and acids ...
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Biobutanol production in a Clostridium acetobutylicum biofilm reactor integrated with simultaneous product recovery by adsorption
... In the fermentation process by C. acetobutylicum , acet- one was not as toxic as butanol. However, selective adsorption of butanol by the KA-I resin could make acetone a predominant inhibitory product in ...
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The significance of aspartate on NAD(H) biosynthesis and ABE fermentation in Clostridium acetobutylicum ATCC 824
... Previous studies have shown that nadA, nadB and nadC were the essential structural genes of the NADH de novo synthesis pathway (Haruhiko et al. 2010; Sun and Setlow 1993; Zhou et al. 2011). In addition, the expression of ...
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Secretion and assembly of functional mini cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824
... for C. acetobutylicum growth on lignocellu- ...in C. acetobutylicum, we are unaware of any attempted expressions of full-length heterologous scaffoldins in ...C. acetobutylicum ...
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Introducing a single secondary alcohol dehydrogenase into butanol-tolerant Clostridium acetobutylicum Rh8 switches ABE fermentation to high level IBE fermentation
... To test the genetic manipulation feasibility of strain Rh8, both methylated and unmethylated shuttle vector pIMP1 were transformed into this mutant strain. The results showed that strain Rh8 could accept the methylated ...
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Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum
... the C. acetobutylicum ATCC824 ...resulting C. acetobutylicum Δcac1502Δupp strain is marker‑less, readily transformable without any previous plasmid methylation and can serve as the host for ...
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Pleiotropic regulation of a glucose-specific PTS in Clostridium acetobutylicum for high-efficient butanol production from corn stover without detoxification
... During batch culture using glucose as the sole carbon source, C. acetobutylicum cells were collected at 8, 16 and 24 h by centrifugation at 10,000×g for 3 min at − 10 °C. The resulting cell pellets ...
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Elucidation of the roles of adhE1 and adhE2 in the primary metabolism of Clostridium acetobutylicum by combining in-frame gene deletion and a quantitative system-scale approach
... early transcriptional termination by the FRT site remain- ing after catP removal was deduced. To confirm the early termination of transcription by an FRT site and to elimi- nate this polar effect on acetone production, a ...
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Enhanced isopropanol–butanol–ethanol mixture production through manipulation of intracellular NAD(P)H level in the recombinant Clostridium acetobutylicum XY16
... of C. acetobutylicum ATCC 824, the final titers of isopropanol, butanol, and etha- nol were improved to ...(C. acetobutylicum ATCC 824 Δbuk pCLF952), the final biofuel titer of ...
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Metabolic flux and transcriptional analysis elucidate higher butanol/acetone ratio feature in ABE extractive fermentation by Clostridium acetobutylicum using cassava substrate
... In addition to strength of the reductive power, many other approaches have been adopted to acquire high butanol/acetone ratio. Among these efforts, some modifi- cations to metabolic pathways were obtained good effect on ...
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Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains
... Metabolic engineering has been used to create pathways for isopropanol production in Escherichia coli . Introduc- tion of four genes from C. acetobutylicum ( ctfA , ctfB , adc and thiolase ( thl )) into E. ...
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Conversion of Carbon Dioxide to Metabolites by Clostridium acetobutylicum KCTC1037 Cultivated with Electrochemical Reducing Power
... Reinforced clostridial (RC) medium (Tryptone 10 g/L, Sodium chloride 5 g/L, Beef extract 10 g/L, Yeast extract 3 g/L, Glucose 20 g/L, Starch 1 g/L, L-cystein hydro- chloride 0.5 g/L, Sodium acetate 3 g/L) was used as a ...
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