C. glutamicum
Engineering cell factories for producing building block chemicals for bio-polymer synthesis
... Although the above-described C. glutamicum and E. coli strains were metabolically engineered to efficiently produce succinic acid, these strains are limited to growth at neutral pH conditions due to their ...
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Ornithine cyclodeaminase-based proline production by Corynebacterium glutamicum
... overproducing C. glutamicum strain [20,21] with deletions of the genes argR and of argF, the gene encoding ornithine carbamoyl-transferase, which converts ornithine to citrulline in the arginine ...
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L-citrulline production by metabolically engineered Corynebacterium glutamicum from glucose and alternative carbon sources
... C. glutamicum has recently been cured of prophage se- quences to yield MB001 (Baumgart et ...in C. glutamicum MB001 yielded the L-arginine auxo- trophic strain CIT0 (Table ...L-arginine, ...
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Systematic pathway engineering of Corynebacterium glutamicum S9114 for l-ornithine production
... l-Ornithine is a derivative product of glutamate, which suggests that increased metabolic flux from 2-oxogluta- rate to glutamate is a reasonable strategy for enhancing l-ornithine production. Therefore, odhA was ...
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Engineering of Corynebacterium glutamicum for growth and production of L-ornithine, L-lysine, and lycopene from hexuronic acids
... of C. glutamicum with 28% protein similarity to that of ...work, C. glutamicum was engineered for the uptake and utilization of D-galacturonate and D-glucuronate as alter- native carbon ...
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Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum strains from empty fruit bunch biosugar solution
... recombinant C. glutamicum H36GD1852 strain was further examined for whether the carbon co-utilization system developed in this study can be efficiently used in the lignocellulosic bio-refinery ...
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Production of the compatible solute α-d-glucosylglycerol by metabolically engineered Corynebacterium glutamicum
... for C. glutamicum a foreign molecule neither synthesized nor consumed by this ...In C. glutamicum the mechanosensitive channel MscCG contributes under hyperosmotic stress conditions to the ...
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Artificial oxidative stress-tolerant Corynebacterium glutamicum
... C. glutamicum strain (KCTC12280BP, i.e., HA strain) showed a distinguished transcriptome pattern (NCBI Gene Expression Omnibus access code: GSE41232). One of the unique transcriptome pattern of the adapted- ...
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Metabolic engineering of Corynebacterium glutamicum for enhanced production of 5-aminovaleric acid
... in-house-developed C. glutamicum shuttle vector pKCA212-MCS was con- structed by cloning the origin of replication of the cryptic plasmid pCC1 [33] into pKA212-MCS at the AatII and XhoI ...in C. ...
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Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
... in C. glutamicum; therefore, we codon-optimized the Cas9 gene and an SD sequence was added in front of the ATG initiation ...to C. glutamicum; therefore, we used the inducible Ptac promoter ...
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Metabolic engineering of Corynebacterium glutamicum for the production of cis, cis-muconic acid from lignin
... Four features have been recently claimed as key to suc- cess in MA bio-production from aromatics: (i) a high CatA activity, (ii) elimination of CatB activity, (iii) robust- ness to aromatics, and (iv) active export of ...
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Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum
... sharp increase in the PtrxB1 activity (Fig. 2c). Very simi- lar results were obtained when C. glutamicum WT was used (data not shown). To detect whether some other stress sigma factor at least weakly ...
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Rich biotin content in lignocellulose biomass plays the key role in determining cellulosic glutamic acid accumulation by Corynebacterium glutamicum
... similar transcription pattern was observed in the biotin rich CSH and in the biotin rich complex medium. The genes involving phospholipid synthesis including cyclo- propane-fatty-acyl-phospholipid synthase gene ufaA ...
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Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum
... were obtained with a mutant efficiency of 70.0% (Fig. 4e). Fig. 4 ssDNA-directed recombineering using CRISPR/Cas9 and RecT in C. glutamicum SL4ΔldhA::rfp. a Schematic representation of pCas9 and pgRNA5 ...
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A RecET-assisted CRISPR–Cas9 genome editing in Corynebacterium glutamicum
... in C. glutamicum expressing the chro- mosome-borne Cas9, suggesting that the introduction of DSB by CRISPR–Cas9 system increased the HR of the damaged DNA as reported elsewhere ...into C. ...
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Biotransformation of ferulic acid to protocatechuic acid by Corynebacterium glutamicum ATCC 21420 engineered to express vanillate O-demethylase
... of C. glutamicum ATCC 21420 (F). The C. glutamicum type strain ATCC 13032 degrades PCA into the TCA cycle intermedi- ates acetyl-CoA and succinyl-CoA via the β-ketoadipate pathway (Shen and ...
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Systems metabolic engineering of Corynebacterium glutamicum for the bioproduction of biliverdin via protoporphyrin independent pathway
... Fig. 1 Schematic representation and thermodynamic data of biosynthetic pathway for production of biliverdin. a Metabolic pathway and main push and pull strategies for biliverdin biosynthesis in C. ...
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Corynebacterium glutamicum as platform for the production of hydroxybenzoic acids
... dihydroxybenzoate. C. glutamicum possesses the 3-dehydroshikimate dehydratase QsuB (Uniprot entry Q8NT86) normally involved in the pathway for quinate and shikimate degradation, which is known to cata- lyze ...
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Engineering Corynebacterium glutamicum for violacein hyper production
... coli, C. glutamicum is generally recognized as safes (GRAS) and exhibits numerous advantages as a micro- bial cell ...factory. C. glutamicum strains dominate indus- trial scale fermentation ...
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Benevides Martins, Gustavo (2019): Subcellular localization and dynamics of transmembrane and membrane associated proteins in Corynebacterium glutamicum. Dissertation, LMU München: Fakultät für Biologie
... Corynebacterium glutamicum Corynebacterium glutamicum is a facultative anaerobic, gram-positive, non- motile, non-sporulating bacterium that in nature, inhabits mainly the surface layers of the ...2012). ...
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