C-terminal heptad repeat
Conserved Salt Bridge between the N- and C-Terminal Heptad Repeat Regions of the Human Immunodeficiency Virus Type 1 gp41 Core Structure Is Critical for Virus Entry and Inhibition
... N-terminal heptad repeat (NHR) (also called HR1), a C-terminal heptad repeat (CHR) (also called HR2), and a tryptophan-rich ...A C peptide known as T20 (brand name, ...
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Six helix bundle completion in the distal C terminal heptad repeat region of gp41 is required for efficient human immunodeficiency virus type 1 infection
... Fig. 3 Fusion assay of alanine insertion mutants of gp41. a and b The DSP assay was performed at 2 h after coculture to examine the fusion activities of mutants. Surface expression levels of mutant Envs were determined ...
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Mutations of Gln64 in the HIV-1 gp41 N-Terminal Heptad Repeat Render Viruses Resistant to Peptide HIV Fusion Inhibitors Targeting the gp41 Pocket
... and C-peptide sequences. FP, fusion peptide; NHR, N-terminal heptad repeat; CHR, C-terminal heptad repeat; TM, transmembrane domain; CP, cytoplasmic ...the c ...
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Nonneutralizing Antibodies Induced by the HIV-1 gp41 NHR Domain Gain Neutralizing Activity in the Presence of the HIV Fusion Inhibitor Enfuvirtide: a Potential Therapeutic Vaccine Strategy
... FIG 2 Schematic representation of the cooperative effect of combining enfuvirtide and NHR-specific antibodies in activity against gp41-mediated membrane fusion. Once gp120 binds to CD4 and a coreceptor such as CCR5, the ...
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Mutations of Glu560 within HIV 1 Envelope Glycoprotein N terminal heptad repeat region contribute to resistance to peptide inhibitors of virus entry
... and C‑terminal heptad repeat regions (HR1 and HR2, respectively) of gp41 can inhibit HIV‑1 infection in a dominant negative manner by interfering with refolding of the viral HR1 and HR2 to ...
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Heptad Repeat 2-Based Peptides Inhibit Avian Sarcoma and Leukosis Virus Subgroup A Infection and Identify a Fusion Intermediate
... distinct heptad repeat domains within the transmembrane ...two heptad repeat domains of the avian sarcoma and leukosis virus subgroup A (ASLV-A) TM subunit of the envelope protein were ...the ...
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The Heptad Repeat C Domain of the Respiratory Syncytial Virus Fusion Protein Plays a Key Role in Membrane Fusion
... Our results point to a stabilizing role for many residues at the N terminus of the helix. Leu-78 and Ile-79 contribute to a hydrophobic belt that lines the inner cavity of the prefusion F trimer, while Lys-75 and Lys-77 ...
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Inhibition of Murine Leukemia Virus Envelope Protein (Env) Processing by Intracellular Expression of the Env N-Terminal Heptad Repeat Region
... with C-helices packing in an antiparallel orientation in the grooves between N-helix ...the C-helix. Peptides from the C-helix of HIV-1 potently inhibit membrane fusion by binding in the grooves of ...
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Oligomerization of the hydrophobic heptad repeat of gp41.
... hydrophobic heptad repeat of gp41 to direct protein oligomerization, we have cloned this region of HIV-1 gp41 into the bacterial expression vector pRIT2T, a plasmid which encodes the N-terminal ...
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Conserved Leucines in N-Terminal Heptad Repeat HR1 of Envelope Fusion Protein F of Group II Nucleopolyhedroviruses Are Important for Correct Processing and Essential for Fusogenicity
... HR regions have been widely detected in membrane fusion proteins of paramyxoviruses, influenza virus, coronaviruses, and retroviruses and have been shown to play an essential role in viral fusion and infectivity (15, ...
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The C Terminus of the B5 Receptor for Herpes Simplex Virus Contains a Functional Region Important for Infection
... 2005). Heptad repeats found in the B5 C terminus are predicted to form an ␣ -helix for coiled coil ...the heptad repeat motif in HSV binding and entry into porcine cells that express B5 and ...
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A Heptad Repeat in Herpes Simplex Virus 1 gH, Located Downstream of the α-Helix with Attributes of a Fusion Peptide, Is Critical for Virus Entry and Fusion
... (iii) A 25-residue peptide corresponding to HR-1 inhibited HSV-1 infection in a dose-dependent manner. The peptide was effective only if present at the time of virus entry into the cell; it was ineffective if present ...
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Common principles and intermediates of viral protein mediated fusion: the HIV 1 paradigm
... (or heptad repeat 1, HR1 domain) and three peripheral C-terminal helices (HR2 domains), as depicted in ...the C- terminal and N-terminal segments of ectodomains of class ...
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Mutational analysis of the leucine zipper-like motif of the human immunodeficiency virus type 1 envelope transmembrane glycoprotein.
... This highly conserved zipper motif, which consists of a heptad repeat of leucine or isoleucine residues, has been suggested to play a role in HIV-1 env glycoprotein oligomerization.. Thi[r] ...
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Residues in the Heptad Repeat A Region of the Fusion Protein Modulate the Virulence of Sendai Virus in Mice
... Recombinant viruses. Recombinant Sendai viruses (rSeVs) of the Enders strain were rescued by reverse genetics (62). Briefly, monolayers of Vero cells in 10-cm dishes were infected with UV-inactivated recombinant vaccinia ...
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Morbitzer, Robert (2013): Erstellung und Analyse von TALE-Transkriptionsfaktoren mit benutzerdefinierter Erkennungsspezifität. Dissertation, LMU München: Fakultät für Biologie
... by repeat-variable diresi- dues (RVDs) of tandem-arranged 34/35-amino acid repeat ...additional repeat units activating specifically either the pepper Bs3 or UPA20 ...of repeat units ...
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Mutational Analysis of Residues in the Coiled-Coil Domain of Human Immunodeficiency Virus Type 1 Transmembrane Protein gp41
... expression. Heptad repeat motifs, characteristic of coiled coils, typically contain bulky hy- drophobic residues that alternate every third and fourth resi- due (the “a” and “d” positions) to create a ...
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Spring-Loaded Heptad Repeat Residues Regulate the Expression and Activation of Paramyxovirus Fusion Protein
... -strands. Heptad repeat a and d residues are shown underneath the sequence ...the heptad repeat (1). (C) Structure of the PIV5 F protein ectodomain in its uncleaved, prefusion form ...
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Bovine leukemia virus long terminal repeat variability: identification of single nucleotide polymorphisms in regulatory sequences
... In support of this concept, Jeeninga et al. revealed that the change from κ B to GABP binding site in the LTR of HIV-1 subtype E did not lead to loss of promoter func- tion; instead, a gain of GABP binding to this ...
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Model of trigger mechanisms of SARS CoV-2 infections
... contains the main protein segments that facilitate virus- cell fusion, including the fusion peptide (FP), two heptad- repeat regions (HR1 and HR2), transmembrane domain (TM[r] ...
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