C. thermocellum
Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition of C. thermocellum by ethanol
... For C. thermocellum, we observed that ethanol tolerance, in experiments with gradual ethanol addition, was twofold higher than previously observed in response to a stepwise increase in the etha‑ nol ...
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Rheological properties of corn stover slurries during fermentation by Clostridium thermocellum
... Clostridium thermocellum (Ruminoclostridium ther- mocellum) are among the most effectively described bio- catalysts at deconstructing cellulosic biomass [22, 28], and produce ethanol and other reduced metabolic ...
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Clostridium thermocellum LL1210 pH homeostasis mechanisms informed by transcriptomics and metabolomics
... to C. thermocellum DSM1313 (NC_017304, last modified 2015/07/30) using Bowtie2 with the same parameters as the very-sensitive preset option expect the number of mismatches was set to 1 [57, 58], and reads ...
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Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes
... organisms with pretreated real biomass, and it is impor- tant to understand whether CBP can realize deconstruc- tion yields competitive with those from conventional pretreatment followed by fungal enzyme hydrolysis and ...
13
Integrated omics analyses reveal the details of metabolic adaptation of Clostridium thermocellum to lignocellulose-derived growth inhibitors released during the deconstruction of switchgrass
... the C. thermocellum genome are illustrated with the red/green darkly shaded regions in the outer ...A, C, D, and E encode various protein products, including NADH dehy- drogenase, ferredoxin, S-layer ...
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Clostridium thermocellum transcriptomic profiles after exposure to furfural or heat stress
... when C. thermocellum was treated with ...The C. thermocellum Cthe_2801 gene encodes a putative carbon monoxide dehydrogenase and this enzyme may play a role in dealing with redox imbal- ance ...
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Consolidated Bioprocessing of Alkaline Pretreated Perennial Grasses Using Clostridium Thermocellum
... of C. thermocellum has a special cellulose binding domain (CBD) which is integrated into the cellulosome with a multitude of enzyme subunits and functions as a point of attachment for the cellulosome to ...
129
Targeted redox and energy cofactor metabolomics in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum
... grew C. thermocellum in a media containing 6 g/L yeast extract [80], which may have both altered metabolite states and, as we found in our work, may have been a source of MS signal suppression, justifying ...
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Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum
... Wild-type C. thermocellum had low ethanol yield and titer and produced large quantities of ...after C. thermocellum sugar catabolism ...
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Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation
... [9–11]. C. thermocellum metabolism is affected by the addition of exogenous fermentation products [12] and inhibitor chemicals [13] as well as other environmen- tal ...which C. thermocellum ...
14
The effect of switchgrass loadings on feedstock solubilization and biofuel production by Clostridium thermocellum
... by C. thermocellum in terms of total soluble sugar utilization (Table ...during C. thermocellum switchgrass fermentations making these compounds unlikely contributors to the switchgrass- ...
9
Direct glucose production from lignocellulose using Clostridium thermocellum cultures supplemented with a thermostable β-glucosidase
... Clostridium thermocellum is an anaerobic, thermophilic, cellulolytic bacterium that produces cellulosomes capable of efficiently degrading plant cell ...of C. thermocellum, it is important to ...
11
Biomass augmentation through thermochemical pretreatments greatly enhances digestion of switchgrass by Clostridium thermocellum
... SBL-2D, Techne, Princeton, NJ) maintained the pretreat- ment temperature within ± 2 °C. The reactor temperature was measured by a K-type thermocouple probe (CAIN- 18G-18, Omega Engineering Co., Stamford, CT, USA). ...
14
Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum
... It is known that CelS plays a key role in cellulose hydrolysis by cellulosome and is the main producer of cellobiose [28, 42], thus the fusion of selected BGL with CelS would greatly release the feedback inhibition ...
14
Expressing the Thermoanaerobacterium saccharolyticum pforA in engineered Clostridium thermocellum improves ethanol production
... that C. thermocellum ferredoxins are compatible with ...into C. thermocellum in this ...and C. thermocellum [4], this ferredoxin seemed to be a reasonable ...
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Functional heterologous expression of an engineered full length CipA from Clostridium thermocellum in Thermoanaerobacterium saccharolyticum
... from C . thermocellum which serve to buffer acid produc- tion ...[11]. C . thermocellum DSM1313 was obtained from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, ...
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The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading
... of C. thermocellum achieved 93% conversion of cellulose (Avicel) initially present at 100 ...for C. thermocellum, including isobutanol, meso- and RR/SS-2,3-butanediol and trace amounts of ...
11
Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum
... of C. thermocellum, and makes large contributions for determining the features of the whole cellulosomal sys- tem, especially in feedback inhibition and catalytic activ- ity ...of C. ...
13
Metabolic engineering of Clostridium thermocellum for n-butanol production from cellulose
... conclusion. For example, Shen et al. found an 18-fold improvement when comparing Bcd-EtfAB to Ter [14]. One possible explanation for the poor performance of Bcd-EtfAB is that it does not interact well with native ...
13
Switchgrass (Panicum virgatum) Fermentation by Clostridium thermocellum and Clostridium beijerinckii Sequential Culture: Effect of Feedstock Particle Size on Gas Production
... of C. thermocellum can be exploited in sequential ...with C. thermo- cellum (63˚C, 48 h) before culturing with ...hoc. C. thermocellum alone produced 78 kPa cumulative pressure ...
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