C2C12 myoblasts
Stimulating effect of graphene oxide on myogenesis of C2C12 myoblasts on RGD peptide-decorated PLGA nanofiber matrices
... addition, C2C12 myoblasts on the PLGA and RGD/PLGA matrices were incubated in the culture media containing 10 μg/ml of GO to examine the effects of ...of C2C12 myoblasts was sig- nificantly (p ...
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PRAS40 Regulates Protein Synthesis and Cell Cycle in C2C12 Myoblasts
... that myoblasts with PRAS40 knockdown had a greater proportion of cells in the G1/G0 phase, compared with scramble controls, and fewer cells in the active S ...in myoblasts retarded cell cycle ...(45). ...
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Clenbuterol Induces Cell Cycle Arrest in C2C12 Myoblasts by Delaying p27 Degradation through β-arrestin 2 Signaling
... promotes skeletal muscle growth mainly by modulating protein synthesis and degradation [5]. In addition, fenoterol enhances skeletal muscle regeneration [43], an ordered progression from the activation of satellite cells ...
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A fractionation method to identify qauntitative changes in protein expression mediated by IGF-1 on the proteome of murine C2C12 myoblasts
... different C2C12 cell preparations were stimulated with IGF-1 (100 ng/ml) for up to 8 hours, fractionated using batch ion-exchange, separated by 2D electrophoresis, and proteins were detected with silver stain ...
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Multiphoton imaging of myogenic differentiation in gelatin-based hydrogels as tissue engineering scaffolds
... the C2C12 myoblasts in the GHPA ...the C2C12 myoblasts in the GHPA hydrogels by the object- ive lens (UPLFLN 10XP, ...the C2C12 myo- blasts in GHPA hydrogels was recollected in ...
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Selection of Muscle-Binding Peptides from Context-Specific Peptide-Presenting Phage Libraries for Adenoviral Vector Targeting
... bind C2C12 cells, this peptide was genetically engineered in between the H and I sheets of the Ad5 fiber, and this modified fiber gene was recombined into a dsRed2 red fluorescent protein-express- ing Ad5 genome ...
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Anti-fatigue activity of a mixture of seahorse (Hippocampus abdominalis) hydrolysate and red ginseng
... The cell proliferation effect of the samples on C2C12 myoblasts was determined by using 5-bromo-2′-deox- yuridine (BrdU) assay (Millipore, Billerica, MA, USA). The cells were seeded at 1 × 10 4 cells per ...
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Increased amyloid β-peptide uptake in skeletal muscle is induced by hyposialylation and may account for apoptosis in GNE myopathy
... C2C12 myoblasts were seeded on gelatin coated coverslips and differentiated as previously described. Lysotracker 75 nM was added for 45 min when specified. After two washes with PBS, cells were fixed with ...
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Stimulated myoblast differentiation on graphene oxide-impregnated PLGA-collagen hybrid fibre matrices
... of C2C12 myoblasts because the oxygen-containing functional moi- eties, including hydroxyl, carboxyl and epoxy groups of the GO may increase the adsorption of serum proteins in cul- ture media ...of ...
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Culturing C2C12 myotubes on micromolded gelatin hydrogels accelerates myotube maturation
... Historically, C2C12 myoblasts have been cultured on uncoated cell culture dishes until they near ...stage, myoblasts are serum withdrawn to induce differentiation and fuse into multinucleated, ...
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Regulation of myotube formation by the actin-binding factor drebrin
... Drebrin is a ubiquitously expressed F-actin side-bind- ing protein that is highly abundant in the brain [25,26]. Drebrin contains an actin-depolymerizing factor II/cofi- lin-like domain, an actin-binding domain and two ...
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Electrically Conductive Polydopamine–Polypyrrole as High Performance Biomaterials for Cell Stimulation in Vitro and Electrical Signal Recording in Vivo
... ABSTRACT: Conductive polymers (CPs) such as polypyrrole (PPY) are emerging biomaterials for use as scaffolds and bioelectrodes which interact with biological systems electrically. Still, more electrically conductive and ...
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Raf kinase inhibitor protein1 is a myogenic inhibitor with conserved function in avians and mammals
... in C2C12 myoblasts inhibits ...of C2C12 myoblasts expressing WT and mutant (S153A and S153/7EE) RKIP proteins c-terminally tagged with GFP, plus a GFP control, were established and assessed ...
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The adipose tissue stromal vascular fraction secretome enhances the proliferation but inhibits the differentiation of myoblasts
... rat myoblasts did not efficiently differentiate into myotubes (see Additional file 1: Figure S1), so we decided to switch to the more commonly used C2C12 cell ...The C2C12 myoblasts cultured ...
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Negative auto-regulation of myostatin expression is mediated by Smad3 and MicroRNA-27
... in C2C12 myoblasts (A) and 48 h differentiated C2C12 myotubes (B) following 12 h treatment with conditioned medium from either control CHO cells (CCM) or from CHO-cells designed to produce and ...
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Ternary Aligned Nanofibers of RGD Peptide-Displaying M13 Bacteriophage/PLGA/Graphene Oxide for Facilitated Myogenesis
... of C2C12 skeletal myoblasts was exceptionally increased on the RGD peptide-containing ...of C2C12 myoblasts on the PLGA/RGD, random GO-PLGA/RGD and aligned GO-PLGA/ RGD nanofiber sheets were ...
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Skeletal myoblast transplantation for repair of myocardial necrosis
... transplanted C2C12 myoblasts into normal mouse hearts and observed no cell junctions be- tween grafted myofibers and host cardiocytes by electron mi- ...proliferating myoblasts have been reported to ...
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Retinoic acid promotes myogenesis in myoblasts by antagonizing transforming growth factor-beta signaling via C/EBPβ
... In silico analysis of the mouse Smad3 gene and the upstream 5-kb region of the predicted promoter re- vealed no canonical retinoic acid response elements (RARE) by which RA could induce transcription by binding to the ...
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miR-1/206 downregulates splicing factor Srsf9 to promote C2C12 differentiation
... 450a-1, a non-myogenic miRNA which does not have overlapping predicted targets with miR-1/206. Luciferase quantification showed that this miRNA did not cross- react with the selected SR 3′ UTRs or with 3′ UTRs car- rying ...
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The normal function of the cancer kinase Mirk/dyrk1B is to reduce reactive oxygen species
... muscle C2C12 myoblasts, but is translocated to the cytoplasm and upregulated when myoblasts initiate differentiation, as shown by immunofluorescence staining and by cell ...contraction. C2C12 ...
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