C2C12 myotubes
Saturated fatty acid palmitate-induced insulin resistance is accompanied with myotube loss and the impaired expression of health benefit myokine genes in C2C12 myotubes
... utilized C2C12 myotubes chronic exposed to palmitate as an insulin re- sistance ...in myotubes, but diversely, oleate can only be metabolized to intracellular FFAs ...
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Agaro Oligosaccharides Prevent Myostatin Hyperexpression and Myosin Heavy Chain Protein Degradation in C2C12 Myotubes Induced by Tumor Necrosis Factor α
... of myotubes exposed to TNF- α . C2C12 myotubes exposed to TNF- α in the presence or absence of ...-induced myotubes than in the TNF- α -free control, possibly resulting in myostatin ...
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Downregulation of lipin-1 induces insulin resistance by increasing intracellular ceramide accumulation in C2C12 myotubes
... Meanwhile, the TAG level was also decreased by lipin-1 silencing in C2C12 myotubes. DAG accumulation in skeletal muscle was associated with the occurrence of insulin resistance, and the reduced DAG contents ...
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Culturing C2C12 myotubes on micromolded gelatin hydrogels accelerates myotube maturation
... culture myotubes for sufficiently long periods of ...prolong C2C12 myotube viability upwards of 21 days post- differentiation [26, ...patterned myotubes not only reach a more mature state but also ...
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Effects of Rhizome Extract of Dioscorea Batatas and Its Active Compound, Allantoin, on the Regulation of Myoblast Differentiation and Mitochondrial Biogenesis in C2C12 Myotubes
... of C2C12 myotubes differentiation and mitochondrial biogenesis regulators were determined by reverse transcriptase (RT)-PCR or Western ...into myotubes and increased energy production through ...
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Impact of acute heat treatment on autophagy and insulin signaling in C2C12 myotubes
... The HT-induced mechanism behind this attenuation may be through the activation of autophagy increased LC3 II and decreased p62 levels, reduction of cellular stress signaling reduced phos[r] ...
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Plastic Additives Decrease Agrin Induced Acetylcholine Receptor Clusters and Myotube Formation in C2C12 Skeletal Muscle Cell Culture
... on C2C12 myotubes and this clustering is increased with agrin treatment ...[18]-[20]. C2C12 cell cultures were switched from GM to DM at 80% confluence, 10 ng/ml agrin was added for the last 16 hours ...
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Acylated and unacylated ghrelin impair skeletal muscle atrophy in mice
... of C2C12 myotubes, which do not express GHSR-1a, protecting them from dexameth- asone-induced atrophy and atrogene ...in C2C12 myotubes (20), the 10-fold lower dexamethasone concentration used ...
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Promoting Effect of Ginsenoside Rb1 for GLUT-4 Gene Expression and Cellular Synthesis in C2Cl2 Muscle Cells
... Ginseng is a most popular herb that its antidiabetic mechanisms have been studied for over a decade. Although stimulation of GLUT-4 redistribution in adipocyte and muscle cells has been described by different ...
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alpha-Actinin interacts with rapsyn in agrin-stimulated AChR clustering
... differentiated C2C12 myotubes, either control or treated, were incubated with soluble recombinant agrin to induce AChR clusters as described previously ...young myotubes (in differentiation medium ...
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Effects of unaltered and bioconverted mulberry leaf extracts on cellular glucose uptake and antidiabetic action in animals
... Insulin-mediated AKT phosphorylation induces GLUT4 translocation from cytosol to membrane, enhancing glu- cose uptake [40, 41]. To confirm this linkage, the effect of two marker compounds, MLE and BMLE on AKT ...
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Disruption of fyn SH3 domain interaction with a proline rich motif in liver kinase B1 results in activation of AMP activated protein kinase
... in C2C12 myotubes. (A) C2C12 myotubes were transducted with either the 11R-LKB1-proline-rich domain (11R-WT) or the 11R-Scrambled LKB1 peptides ...(D) C2C12 myotubes were ...
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Telmisartan ameliorates insulin sensitivity by activating the AMPK/SIRT1 pathway in skeletal muscle of obese db/db mice
... Total RNA was isolated from C2C12 myotubes using RNAiso plus (Takara Bio Inc., Shiga, Japan). Complemen- tary DNA synthesis was performed, using a QuantiTect W Reverse Transcription kit (SA Biosciences, ...
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Myotubularin controls desmin intermediate filament architecture and mitochondrial dynamics in human and mouse skeletal muscle
... MTM1 binds desmin IFs in muscle. To decipher the muscle-spe- cific role of MTM1 and the physiopathological mechanisms of XLCNM, we performed a yeast 2-hybrid (Y2H) screen using the MTM1 sequence as bait against a human ...
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A differential response to newt regeneration extract by C2C12 and primary mammalian muscle cells
... A1 myotubes show that serum stimulation results in cell cycle re-entry ...mammalian C2C12 muscle cell line [16]. C2C12 myotubes undergo dedifferentiation in culture in response to fusion with ...
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Increased amyloid β-peptide uptake in skeletal muscle is induced by hyposialylation and may account for apoptosis in GNE myopathy
... GNE myopathy is an autosomal recessive muscular disorder of young adults characterized by progressive skeletal muscle weakness and wasting. It is caused by a mutation in the UDP-N-acetylglucosamine ...
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Prion Infection of Muscle Cells In Vitro
... in C2C12 myo- blasts and ...of C2C12 myoblasts (A) and D5 myotubes (B) are illustrated by immunofluorescence stain- ing for the cytoskeletal protein ...protein), C2C12 myoblasts (126 g ...
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Palmitoleic acid prevents palmitic acid-induced macrophage activation and consequent p38 MAPK-mediated-skeletal muscle insulin resistance
... into myotubes was achieved by switching to DMEM containing 2% horse serum for 5 ...to C2C12 myotubes for a further 16 h. Myotubes were then serum-starved for 2 h and selected wells stimulated ...
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Induction of myasthenia by immunization against muscle specific kinase
... antibodies could be used to detect MuSK in Western blots, a chi- meric protein that combined the MuSK ectodomain with human placental alkaline phosphatase (MuSK-AP) was transfected into COS-7 cells. As expected, proteins ...
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Raf kinase inhibitor protein1 is a myogenic inhibitor with conserved function in avians and mammals
... as C2C12 cultures overexpressing WT and S153A RKIP produce few myotubes the failure to activate ERK, which occurs in myotubes (Fig 5C,E), could merely reflect an inability to differentiate rather ...
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