C6/36 cell culture
Dengue Virus Detection Using Whole Blood for Reverse Transcriptase PCR and Virus Isolation
... direct C6/36 cell culture and mosquito inoculation was enhanced by using whole blood but not to the same extent as that seen by the use of ...
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Purification of Dengue Virus Particles by One Step Ceramic Hydroxyapatite Chromatography
... The C6/36 cells were cultured in Minimum Essential Medium Eagle (modified) (modified EMEM, MP Biomedicals, Irvine, CA, USA) containing 10% fetal bovine serum (FBS; Invitrogen Corporation, Carlsbad, CA, USA) ...
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Identification and Characterization of Genetically Divergent Members of the Newly Established Family Mesoniviridae
... on C6/36 (Aedes albopictus) and Vero cells as described previously (1, ...on C6/36 cells were performed using in- fectious cell culture supernatant from the 2nd passage (isolates ...
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Sindbis virus interferes with dengue 4 virus replication and its potential transmission by Aedes albopictus
... in cell culture but several mechanisms have been ...host cell replication sites or substrate necessary for viral replication [7], virus-directed intracellular mechanisms [16], production of defective ...
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Differential Incorporation of Cholesterol by Sindbis Virus Grown in Mammalian or Insect Cells
... Cell culture, plaque assay, and virus purification. BHK-21 and mosquito cell culture, plaque assay, and virus purification are described in detail in reference ...U4.4 cell line, ...
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Increased Production of Interleukin-8 in Primary Human Monocytes and in Human Epithelial and Endothelial Cell Lines after Dengue Virus Challenge
... control cell lines were grown to 50% confluence in 35-mm- diameter tissue culture ...insect cell medium (C6/36), uninfected or infected Vero cell antigen preparation (50 ...
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S-Phase-Dependent Enhancement of Dengue Virus 2 Replication in Mosquito Cells, but Not in Human Cells
... cycling C6/36 cells were infected with DEN2 strain N1042, with a low-passage DEN2 Thai isolate (C0477), or with a high-passage DEN2 strain (16681), washed at 2 hpi, and then treated with PBS or ...tissue ...
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A novel RGDyC/PEG co-modified PAMAM dendrimer-loaded arsenic trioxide of glioma targeting delivery system
... and C6 cell co-culture models were established to investigate the inhibitory effect of different ATO formulation after transporting across ...by C6 cells. The results of cell cycle and ...
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The Host Range Phenotype Displayed by a Sindbis Virus Glycoprotein Variant Results from Virion Aggregation and Retention on the Surface of Mosquito Cells
... of culture medium were collected, clarified by microcentrifu- gation, and stored at ⫺70°C (preagitation ...of cell suspension was viewed under an inverted microscope to assess cell integrity (intact ...
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Human T Lymphocytes Are Permissive for Dengue Virus Replication
... Viral stocks and infection of T lymphocytes. DV infection assays were performed with all four serotypes. Two strains, DV1/BR/90 (GenBank accession number S64849.1), first described by Desprès and colleagues in 1993 (44), ...
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Sequence analysis of the small (s) RNA segment of viruses in the genus Orthobunyavirus
... different cell lines until a good cpe was ...the cell culture systems (more than 5 passages) were ...the cell culture systems (Vero-E6, BHK-21 and LLCMK2), I managed to grow 27 of the ...
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The insect specific Palm Creek virus modulates West Nile virus infection in and transmission by Australian mosquitoes
... CxFV, Culex flavivirus; DENVs, dengue viruses; EDTA, ethylenediaminetetraacetic acid; FBS, fetal bovine serum; GM, growth media; IHC, immunohistochemistry; ISF, insect specific flavivirus; Jak-STAT, Janus kinase-signal ...
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The role of glycogen synthase kinase-3β in glioma cell apoptosis induced by remifentanil
... Therefore, GSK-3β is now considered as a therapeutic target for cancer treatment [17, 34]. Modulation of GSK-3β can markedly increase p53-dependent activation of Bax, leading to cytochrome C release, loss of ...
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Comparison of three commercially available amplification assays, AMP CT, LCx, and COBAS AMPLICOR, for detection of Chlamydia trachomatis in first void urine
... The differences between the sensitivities of the three ampli- fication assays are not due to a difference in initial accuracy between the assays, since we diluted several C. trachomatis- positive patient samples with a ...
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Screening for differentially expressed miRNAs in Aedes albopictus (Diptera: Culicidae) exposed to DENV 2 and their effect on replication of DENV 2 in C6/36 cells
... Previously, we constructed three small RNA libraries from the midguts of Ae. albopictus females that were either fed sugar solution, a regular blood meal, or a blood meal infected with DENV-2. We then investigated the ...
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Does Japanese encephalitis virus share the same cellular receptor with other mosquito borne flaviviruses on the C6/36 mosquito cells?
... the cell surface, and (ii) receptors that are used by the virus after binding to the cell, which are referred to using different terminology, such as post-binding, post-attach- ment, entry, fusion, ...
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Asymmetric Requirement for Cholesterol in Receptor-Bearing but Not Envelope-Bearing Membranes for Fusion Mediated by Ecotropic Murine Leukemia Virus
... Detergent solubility assays. To see if MLV envelope is raft associated, we treated BHK cells expressing MLV envelope with 1% Trition X-100 at 4°C. This treatment solubilizes non- raft proteins but leaves raft proteins ...
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Antitumor activity of dichloroacetate on C6 glioma cell: in vitro and in vivo evaluation
... For cell cycle analysis, the pellets were suspended in ...for cell cycle flow cytometry was 10,000. Cell cycle profiles were studied using a flow cytometer (FACSCalibur; Becton Dickinson, Mountain ...
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Vaccinia Virus Protein C6 Is a Virulence Factor that Binds TBK 1 Adaptor Proteins and Inhibits Activation of IRF3 and IRF7
... and C6 are evidently not duplicative, because when K7 is still expressed, loss of C6 caused a marked virus attenuation in two models of ...the cell type infected or stage of infection, thus requiring ...
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Exclusion of superinfecting homologous virus by Sindbis virus-infected Aedes albopictus (mosquito) cells.
... 1, yields of superinfecting SVHR virus capable of forming a plaque at the nonpermissive temperature from both C6/36 and C710 cells were reduced when superinfection was performed at 10 an[r] ...
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