Capsid assembly
Involvement of a Nonstructural Protein in Poliovirus Capsid Assembly
... facilitate capsid assembly, e.g., the capsid-associated protein 80 (p80) of African swine fever virus (50), the T antigen (TAg) of simian virus 40 (SV40) (51), and the nonstructural protein 40 ...
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Formation of RNA Granule-Derived Capsid Assembly Intermediates Appears To Be Conserved between Human Immunodeficiency Virus Type 1 and the Nonprimate Lentivirus Feline Immunodeficiency Virus
... Many viruses are known to utilize RNA granules at various stages of their life cycles (71). Canonical RNA granules include stress granules and P bodies, both of which are much larger than the ABCE1- and DDX6-containing ...
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Feline Calicivirus Capsid Protein Expression and Capsid Assembly in Cultured Feline Cells
... FCV capsid assembly. Transfection of both the precursor capsid protein gene and a truncated (mature) capsid protein gene resulted in the expression of the respective capsid pro- teins, ...
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Involvement of a Nonstructural Protein in Poliovirus Capsid Assembly
... facilitate capsid assembly, e.g., the capsid-associated protein 80 (p80) of African swine fever virus (50), the T antigen (TAg) of simian virus 40 (SV40) (51), and the nonstructural protein 40 ...
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Surface Loop Dynamics in Adeno-Associated Virus Capsid Assembly
... ⫺/⫺ capsid VPs were expressed (data not shown) but were unable to assemble into particles, as determined by A20 Western dot blot analysis ...the capsid were suggested by the ring-like staining; therefore, ...
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The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes
... the assembly process takes place ...for capsid formation. A role of the nucleolar capsid-interacting proteins in the assembly process has not been analyzed thus ...during assembly and ...
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RNA and Nucleocapsid Are Dispensable for Mature HIV-1 Capsid Assembly
... Human immunodeficiency virus type 1 (HIV-1) is released from infected cells in an immature, noninfectious form in which the structural polyprotein Gag is arranged in a hexameric lattice, forming an incomplete spherical ...
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Intragenic suppression of a capsid assembly-defective P22 tailspike mutation.
... From the analysis of the hmH3034 mutant and its suppressors, we propose that in the wild-type tailspike protein, the Asp residue at position 100 and the Arg residue at posit[r] ...
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Involvement of a Nonstructural Protein in Poliovirus Capsid Assembly
... 142 Effects of 2Apro mutations I99V/G102R on PV-1 polyprotein processing 143 Since the proteolytic activity of 2Apro on the truncated P1-2A polyprotein was affected by the 144 selected 2[r] ...
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Picornaviral capsid assembly: similarity of rhinovirus and enterovirus precursor subunits.
... The cell debris was 548 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Cytoplasmic extracts of rhinovirus lA-infected HeLa cells, pulsed 15 min with [3H]leucine, conta[r] ...
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Residues on Adeno-associated Virus Capsid Lumen Dictate Interactions and Compatibility with the Assembly-Activating Protein
... icosahedral capsid containing only the ...structural capsid monomer (4); VP2 and VP1 contain the entire VP3 sequence and same structural fold, but extend N-terminally by ⬃ 65 and ⬃ 202 residues, ...
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Structural transitions during the scaffolding driven assembly of a viral capsid
... three capsid states reveal distinctive structural changes exposing the transient steps of the maturation process related specifically to SPs release and DNA ...three capsid states were traced de novo (see ...
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In Vitro Assembly of an Empty Picornavirus Capsid follows a Dodecahedral Path
... empty capsid assembly in vitro, we explored conditions under which pentamers could assemble to complete empty capsid in the absence of defective assembly or ...empty capsid, we started ...
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Self-Assembly of Epstein-Barr Virus Capsids
... the assembly pathway and high-resolution structural analysis of the capsid have not been determined for this ...EBV capsid assembly, the baculovirus expression vector (BEV) system was used to ...
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Assembly of the Herpes Simplex Virus Capsid: Preformed Triplexes Bind to the Nascent Capsid
... inhibited capsid assem- bly could be due to the absence of sequences required for triplex formation or to the lack of sequences involved in at- tachment of the triplex to the major capsid-scaffold protein ...
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The Apical Region of the Herpes Simplex Virus Major Capsid Protein Promotes Capsid Maturation
... VP26 assembly on ...that capsid assembly occurred but is ...of capsid hexamers ...each capsid species, similar to the wild type ...
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Assembly of Adenoviruses
... The mechanism of bacteriophage head assembly, originally thought from electron microscope data 6 to involve a capsid assembly on the outside of DNA condensates, has recently been reinter[r] ...
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The Cellular Chaperone Heat Shock Protein 90 Is Required for Foot-and-Mouth Disease Virus Capsid Precursor Processing and Assembly of Capsid Pentamers
... nism. Capsid precursors are then processed by the viral 3C or 3CD protease, facilitating their multimerization into a pentameric capsid subunit and encapsidation of the viral RNA to form virions or empty ...
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Full-Length Hepatitis B Virus Core Protein Packages Viral and Heterologous RNA with Similarly High Levels of Cooperativity
... containing capsid protein were then passed over a 60-ml Blue Sepharose HP (GE Health- care) column equilibrated in purification ...containing capsid were dialyzed against purification ...
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A Proteomic Atlas of the African Swine Fever Virus Particle
... At least seven known membrane viral proteins (p17 [pD117L], pE183L, p12 [pO61R], p22 [pKP177L], pH108R, pE199L, and pE248R) localize at the inner envelope, while only one viral protein, the CD2 homologue pEP402R, has ...
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