carboxypeptidase A
Cloning and characterization of the novel gene for mast cell carboxypeptidase A
... cell carboxypeptidase has previously been ...cell carboxypeptidase A (MC-CPA) is a prominent secretory granule marker of mast cell differentiation and ...
11
Glutamate carboxypeptidase activity in human skin biopsies as a pharmacodynamic marker for clinical studies
... Background: Glutamate excitotoxicity is thought to be involved in the pathogenesis of neurodegenerative disease. One potential source of glutamate is N-acetyl-aspartyl-glutamate (NAAG) which is hydrolyzed to glutamate ...
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The crystal structure and oligomeric form of Escherichia coli L,D carboxypeptidase A
... Bacterial peptidoglycan is constructed by cross-linking sugar chains carrying pentapeptide building blocks with two D-alanine residues at the C-terminus. Incorporation into the polymer and subsequent breakdown of ...
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Human mast cell carboxypeptidase Purification and characterization
... from carboxypeptidase A in its inability to hydrolyze des-Arg9 ...cell carboxypeptidase was similar to the composition of rat mast cell ...cell carboxypeptidase demonstrated 65% positional identity ...
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ACE INHIBITORS: A COMPREHENSIVE REVIEW
... The potency of the drugs depends on the affinity of the various drugs towards the zinc binding site. The carboxyl group containing derivatives tends to be most potent of the five classes. Also the ACE inhibitor activity ...
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Malting characteristics of some nepalese finger millet (eleusine coracana) varieties
... Beta amylase activity in native millets was in the range of 0.1 – 2.4 units but the values were not significantly different. Beta amylase activities in 48 h germinated millet malts were 357.4, 385, 360.2, 244.9, 349.2 ...
6
Brennfleck, Frank Werner (2018): Etablierung eines Tetrazyklin-induzierbaren Genexpressions-Systems zur Untersuchung des Ras-Guaninnukleotid-Austauschfaktors 4 in der humanen Mastzelllinie HMC-1. Dissertation, LMU München: Medizinische Fakultät
... eine murine Isoform des RasGRP4 und konnten durch deren transiente Überexpression in HMC-1 Zellen eine Zunahme der. Tryptase-, Chymase- und Carboxypeptidase A-Expression induzieren[r] ...
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Translocated duplication of a targeted chromosomal segment enhances gene expression at the duplicated site and results in phenotypic changes in Aspergillus oryzae
... Total protease, alpha-amylase, and acid carboxypeptidase activity in solid-state cultures; RIB40 (control), J4 (the strain bearing translocated duplication of chromosome 2), I-8 (the st[r] ...
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TAFI: A Potential Target for Fibrinolytic Drugs
... The most important technique of enzyme inhibition is either binding of the enzyme to an inhibitor or proteolytic degradation of the enzyme (often by its activator). TAFIa binds to the well-known plasma inhibitor alpha-2 ...
12
In vivo regulation of plasminogen function by plasma carboxypeptidase B
... total carboxypeptidase and CpN in the presence of PCI accord- ing to the manufacturer’s ...total carboxypeptidase activity and the CpN activity represents activated ...
9
Glucarpidase to combat toxic levels of methotrexate in patients
... Drug Administration for intravenous treatment of toxic plasma methotrexate concentrations due to impaired renal clearance. Methotrexate, an antifolate agent, has been used for over 60 years in the treatment of various ...
11
Comparative two time-point proteome analysis of the plasma from preterm infants with and without bronchopulmonary dysplasia
... Results: Children with BPD were characterized by decreased afamin, gelsolin and carboxypeptidase N subunit 2 levels in cord blood, and decreased galectin-3 binding protein and hemoglobin[r] ...
9
Investigations into the enzymology and biotechnology of the hyperthermophilic carboxypeptidase (PfuCP) from the Archaeon Pyrococcus furiosus
... The broad amino acid specificity and rapid digestion by PfuCP in peptide sequencing trials show promise, and high-temperature protein sequencing has now been demonstrated for the first t[r] ...
13
Silencing of carboxypeptidase E inhibits cell proliferation, tumorigenicity, and metastasis of osteosarcoma cells
... Abstract: Carboxypeptidase E (CPE), a prohormone processing enzyme, has been implicated in the progression of multiple malignancies. However, the biological role and molecular mecha- nisms of CPE in osteosarcoma ...
9
Characterisation of the carboxypeptidase G2 catalytic site and design of new inhibitors for cancer therapy
... enzyme carboxypeptidase G2 (CPG2) is utilized in antibody directed enzyme pro-drug therapy (ADEPT) to catalyze the formation of an active drug from an inert ...
12
Increased secretory demand rather than a defect in the proinsulin conversion mechanism causes hyperproinsulinemia in a glucose infusion rat model of non insulin dependent diabetes mellitus
... and carboxypeptidase-H [CP-H]) and proinsulin, were examined in islets isolated from 48-h infused rats with 50% (wt/vol) glucose (hyperglycemic, hyperinsulinemic, and increased pancreatic proinsulin to insulin ...
9
Characterization of MB creatine kinase isoform conversion in vitro and in vivo in dogs
... plasma carboxypeptidase N produces two additional isoforms that are readily separated, thereby permitting sensitive, early detection of acute myocardial ...
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PEP4 GENE FUNCTION IS REQUIRED FOR EXPRESSION OF SEVERAL VACUOLAR HYDROLASES IN SACCHAROMYCES CEREVISIAE
... The pep4-3 mutation results in a 90-9570 reduction in the levels of five vacuolar hydrolases in yeast, including proteinases A and B, carboxypeptidase Y , RNase(s) and t[r] ...
13
The Effects of Acetylenic Tricyclic Bis-(Cyano Enone) on Cell Migration
... The acetylenic tricyclic bis(cyano enone), TBE-31 inhibits non-small cell lung cancer cell migration through direct binding with actin. Association of tubulin carboxypeptidase with mi[r] ...
247
Biophysical study into the structure and substrate binding properties of peptido mimetic ligands to carboxypeptidase G2
... The members of the M20 family are known to be aminoacylases or exopeptidases, such as CPG2 (Levy and Goldman 1967). Many of these enzymes have shown potential as therapeutic agents. Succinyldiaminopimelate ...
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